OBJECTIVE：To observe the therapeutic effect of limaixian in treating Helicobacterium pyloricus(HP)-related gastroduodenopathy of childhood and evaluate its role playing in triple short-term therapeutic scheme(TS scheme).METHODS：153 patients，who had definitely been diagnosed as HP-related gastroduodenopathy by gastroscopy or 13 C-UBT，were randomly divided into three groups(A，B，C)receiving ST scheme(omeprazole as the main drug).Group A：omeprazole 0.8mg/(kg*d)，metronidazole 20mg/(kg*d) and amoxycillin 40mg/(kg*d)； Group B omeprazole，metronidazole and klacid 15mg/(kg*d)； Group C：omeprazole，metronidazole and limaixian 15mg/(kg*d).Therapeutic course was 2 wks and gastroscopy or 13C-UBT were carried out 4 wks after discontinuation of drugs.RESULTS：(1)HP eradication rates of group A，B and C were 62.7％，92.1％ and 94.1％ respectively.The rates of group B,C were higher than that of group A.There were significant differences among three groups(X2=31.8,P<0.01).Group B showed no significant different with group C.(2) The remission rates of pain of group B and C were markedly higher than that of group A one and two wks after treatment (X2=19.3,P<0.05),there was no significant difference between group B and C.CONCLUSION:As an anti－ HP infection agent in TS scheme,limaixian is better than amoxycillin in therapeutic effect.Limaixian is comparable with imported klacid and inexpensive in price,it can be used as the first choice antibiotic in TS scheme.

In the present work the relative biological availability (RBA) of iron in the blood and the liver paste of swine has been studied by means of the hemoglobin regeneration technique in chickens. One day-old chickens were first made anemic on a low iron basal diet for 3 weeks, and were then divided into groups to which basal diets with various iron levels (10, 20, 40 ppm Fe) from different iron sources (ferrous sulfate reference standard, whole blood meal of swine and liver paste of swine) were subsequently fed respectively.After 16 days on test diets hemoglobin levels and hematocrit values in the blood of the chickens were measured. The chickens were then sacrificed for measurement of the iron concentration in their livers.Regression lines of each iron source were fitted by plotting final hemo- globin concentrations against the corresponding iron concentrations of the diet fed. Let the slope of the regression line from ferrous sulfate be 100, (i.e. relative bioavailability of ferrous sulfate), the RBA of the other 3 iron sources was as follows; processed whole blood meal of swine was 90.2; raw blood meal of swine 64.6; and the live paste of swine 78.4; but the differences between any two of them are statistically non-significant; i.e. their effects on hemoglobin regeneration are of the same extent.

Objective To review the relation between alpha fetoprotein(AFP) and gastric carcinoma as well as the advancement of literatures about the alpha fetoprotein-producing gastric carcinoma(AFPGC).Methods Domestic and foreign literatures on AFPGC were collected and reviewed.Results AFP is very useful to the diagnosis,pathological type and prognosis of the AFPGC as a specific tumor marker.Conclusion AFPGC is a special type of gastric cancer with high degree of malignancy and poor prognosis.

With food containing konjac flour, a 45-day-long feeding trial on 110 old people suffering from hyperlipidemia was conducted to observe the effect of konjac food on lipid metabolism of human beings.The konjac flour was from Amorphophallus konjac, a kind of traditional food, with trace protein and very low energy, but rich in dietary fibre composed mainly of gluco-mannan.The results obtained were as follows;1.After having konjac flour 5g/day as a supplement of their regular meal for 45 days, concentration of TG, TC and LDLC in the serum of the experimental subjects decreased significantly (P

In order to evaluate the availability of the lateral horizontal laryngectomy and anaplasty of epiglottis to treat some patients with specific supraglottic carcinomas and hypopharyngeal carcinomas, 17 cases of laryngeal and hypopharyngeal carcinomas were retrospectively analyzed, whose tumors were located at the lateral margin of epiglottis, aryepiglottic fold, medial wall of piriform fossa and were treated by the lateral horizontal laryngectomy and anaplasty of epiglottis. The results showed that all cases took food by mouth in postoperative 9-14 days and subjected to decannulation in postoperative 9-15 days. Three cases had postoperative hoarse voice. The free-disease survival rate of 3 years was 71.4% in 14 cases followed up after the first surgical therapy, and the overall free-disease survival rate of 3 years was 85.7% after the second surgical therapy. It was concluded that the manipulations of the lateral horizontal laryngectomy and epiglottiplasty were simple. It could alleviate the postoperative symptoms of aspiration and bucking remarkably and shorten their postoperative recovery time, yet does not lower the survival rate of patients if laryngocarcinoma or hypopharyngeal carcinoma cases were properly selected.
Carcinoma, Squamous Cell/*surgery ; Epiglottis/*surgery ; Hypopharyngeal Neoplasms/surgery ; Laryngeal Neoplasms/*surgery ; Laryngectomy/*methods ; Otorhinolaryngologic Surgical Procedures/methods

In the present article the effects of animal blood and liver on the status of iron nutrition in preschool children were studied.71 apparently normal children in a kindergarten, aged 3-7 years, were selected for feeding trial. All of them were undergone physical examination to verify the absence of hookworm infestation and chronic diseases, but having a bit lower hemoglobin concentration (Hb

Seventy two diabetic subjects type Ⅱ were given konjac food for 65 days.The data analysed by multiple F-test indicated that the fasting blood glucosc(FBG), 2-h postprandial blood glucose (PBG) at the 30th and 65th day after consuming the food were significantly reduced (P = 0.001, P200mg%) decreased on the average by 51.8 and 84.6 mg% respectively, those with FBG-O 150-200 mg% by 24.1 and 68.7mg%, and those with FBG-0200 mg%).It could be concluded that konjac food was very useful in both prevention and treatment of hyperglycemia.

OBJECTIVETo examine the prevalence and the sequence of the genes of new genotypes of hepatitis G virus (HGV) in Guangxi, China.

METHODSSerum samples were collected from 85 intravenous drug abusers (IVDAs), 80 patients with liver diseases (PLDs) and 50 blood donors (BDs). All sera (n=215) were tested by using EIA for HBsAg, anti-HCV and anti-HIV, and by using nested PCR for HGV RNA. In 62 subjects positive for HGV, HGV RNA was sequenced, and a phylogenetic tree was constructed for analyzing genotypes of HGV.

RESULTSHGV RNA was detected in 85 of 215 serum samples (39.53%). The positivity rates for HBsAg, anti-HCV and anti-HIV were 39.07%, 42.79% and 0, respectively. First, 11 nucleotide sequences were determined and the isolates were grouped into three clusters with HGV. 5 of 11 HGV isolates clustered in a distinct phylogenetic branch (genotype Asia) which was different from the described GBV-C and HGV sequences, suggesting the presence of a new genotype of HGV in this locality. Second, 51 nucleotide sequences were determined and analyzed for their genotypes of HGV, and showed genotype GBV-C (3.23%), genotype HGV 30-65% and new genotype (genotype Asia) 64.51%, respectively.

CONCLUSIONSThere were subgenotypes in 3 genotypes of HGV; The predominant genotypes of HGV were genotype Asia and genotype HGV among IVDAs, PLDs, and BDs patients in Guangxi, China.

Adult ; Blood Donors ; China ; epidemiology ; Female ; GB virus C ; genetics ; isolation & purification ; Genotype ; Hepatitis C ; epidemiology ; Humans ; Liver Diseases ; virology ; Male ; Polymerase Chain Reaction ; RNA, Viral ; genetics ; Sequence Analysis, RNA ; Substance Abuse, Intravenous ; virology

Human kallikrein-1 (hK1) gene was cloned from kidney tissues cDNA, it was inserted into the plasmid pPICZalphaA, then the yeast expression vector pPICZalpha-hK1 was constructed. After transformed into Pichia pastoris host X33, high-level expression transformants were screened by escalating the concentration of Zeocin (from 500 to 700 microg/mL) of YPD plate and medium. When temperature was 30 degrees C, pH 6.0 with induction duration of 64 hours in the 30 L fermenter, the highest yield can reach about 6500 u/L (1.25 g/L). The variation of glycosylation resulted in two kinds of molecules, i.e. rhK1-H with a heavy molecular weight and rhK1-L with a light one. rhK1 was purified from the supernatant through Phenyl hydrophobic interaction, Cu(2+)-charged Chelating and Anion-exchange chromatography. 0.28 g rhK1-H and 0.62 g rhK1-L can be purified from one liter supernatant. The yield recovery was 72% with a purity of > 96%. So far our yield of rhK1 is superior than known recombinant expression method reported by other researchers.
Amino Acid Sequence ; Base Sequence ; Chromatography, Ion Exchange ; methods ; Genetic Vectors ; genetics ; Humans ; Kidney ; metabolism ; Molecular Sequence Data ; Pichia ; genetics ; metabolism ; Recombinant Proteins ; biosynthesis ; genetics ; isolation & purification ; Tissue Kallikreins ; biosynthesis ; genetics