OBJECTIVE To explore prevention and control measures and disinfection methods of intestinal virus hospital infection.METHODS Based on the prevention and control of hand-foot-and-mouth disease(2009),the measures on the administration of hospital infection and disinfection techniqul were taken,under the guidance of the center for disease control.RESULTS The effective prevention and control in intestinal virus stopped the spread and cross-infection.CONCLUSIONS Countermeasures of intestinal virus diseases caused by toxic encephalitis are effective following HFMD hospital infection prevention and control measures.

Objective To investigate the effects of different carrier gases on the performance of Ohmeda Tec6 desflurane vaporizer. Methods Forty ASA Ⅰ-Ⅱpatients undergoing surgery under general anesthesia with desflurane were enrolled in this study. The age ranged from 37-67 years(mean age 60 years) and body weight from 46-75kg(mean body weight 59.6kg). After induction of anesthesia and tracheal intubation anesthesia was maintained with desflurane. The fresh gas flow(FGF) rate of Ohmeda 210 anesthesia machine was maintained at 5.0L/min and vaporizer dial was set at 6% or 3% . The inspired Concentration of desflurane(FIdes) was analyzed by a Datex ULT-V Capnomac. Five different carrier gases were used:(1) 100%O2;(2) 70% O2 + 30%N2O;(3) 30% O2 + 70%N2O;(4) 70% O2 + 30% N2;(5)30% O2 + 70% N2. There was an interval of 10 mm between two different carrier gases and 40 samples were analyzed for each carrier gas. Results Different carrier gases markedly affected the outflow of Tec6 desflurane vaporizer. The inspired concentration of desflurane decreased significantly with the decrease in oxygen concentration of FGF. Nitrous oxide affected FIdes more significantly than nitrogen at the same concentration of oxygen. Conclusions The oxygen and nitrous oxide concentration of carrier gas affects the inspired concentration of desflurane when Ohmeda Tec6 desflurane vaporized is used.

OBJECTIVE To analyze 61 cases occupational exposure risk factors,the preventive measures and reduce occupational exposure risk.METHODS The record of 61 cases of occupational exposure such as job seniority,occupation,exposed location,place,method,damage degree,processing,preventive medicine and follow-up results register will be statisticully analysed according to the registered items from Jan 2007 to Dec 2008.RESULTS The factors of occupational exposuve were needle treatment,violating operution standard and deficient of protective measures.Both ward and hand were the main place of occupational exposure.CONCLUSIONS Strengthening of effective preventive measues is key to minimize the risk of occupational exposure and reduce the incidence of infection.

Objective To study the clinical characteristics,pathology,and treatment for papillary carcinoma of the breast.Methods The clinical data of 17 patients of papillary carcinoma of the breast admitted in the First Affiliated Hospital of Wen Zhou Medical College were retrospectively analyzed.Results Papillary carcinoma of the breast accounted for 0.64％ of all breast cancer cases hospitalized during last 10 years.All cases had palpable lumps in the breast.12 cases received modified radical mastectomy,2 cases received simple mastectomy,2 cases underwent breast conservation therapy,1 case underwent simple mastectomy plus sentinel lymph node biopsy.15 patients received postoperative chemotherapy,among those 5 cases also received radiotherapy.During a 32.5-month median follow-up ( 1 month to 8 years),one case with bone metastases died 2 years postoperatively and another one died of multimetastases 7 years later.Conclusions The prognosis of papillary carcinoma of the breast is closely related with its pathology type.For intraductal papillary carcinoma low-traumatic therapy is applicable,while in case of infiltrating papillary carcinoma or invasive micropapillary carcinoma ( IMPC ),more aggressive therapies like that adopted for infiltrating ductal carcinoma are recommended.

ObjectiveTo determine whether aphasia has effect on the degree of cognitive disorder for the patients with brain injury, so that prognosis can be determined. MethodsThere are 35 cases of patients with brain injury and they are divided into two groups by aphasia screening test with aphasia and without aphasia. Those with cognitial capacity screening examination(CCSE) and the Loewenstein Occupational Therapy Cognitive Assessment Battery(LOTCA) are examined. ResultThe result is that there is significant differences between these two groups of patents. ConclusionThe patients with brain injury who complecated with aphasia had worse cognitive disorder.

Objective To investigate the effects of antisense recombinant euraryotic expression vector of HCCR-2 on the proliferation and apoptosis of HepG2. Methods The antisense recombinant eukaryotic expression vector of HCCR-2 was constructed. The vector was stably transfected to the HepG2 cells, and positive clones were selected by G418 (antiseuse vector group), pIRES2-EGFP vector was transfected into the HepG2 cells in the same way (pIRES2-EGFP group). The conditions of the nontransfected HepG2 cells were used as control (HepG2 group). Changes in cell growth curve, cell cycle, cell apoptosis and morphology of HepG2 cells after the transfec-tion were detected by MTT method, flow cytometry and transmission electron microscopy, respectively. All the data were analyzed by one-way ANOVA and chi-square test. Results The expression level of HCCR-2 mRNA was down-regulated to 0.39±0.04 in antisense vector group, and the expression level of HCCR-2 mRNA in pIRES2-EGFP group and HepG2 group were 0.62±0.06 and 0.72±0.03, respectively, with significant difference among the 3 groups (F=43.701, P<0.05). The apoptotic rate of HepG2 cells in antisense vector group, pIRES2-EGFP grop and HepG2 group were 13.30%, 2.51% and 2.07%, respectively, with significant difference among the 3 group (χ2=6.793, 8.721, P<0.05). The growth of HepG2 cells in antisense vector group was retarded, and was blocked in G0/G1 stage. Conclusions The HCCR-2 antisense recombinant eukaryotic expression vector can inhibit the mRNA expression of HCCR-2 and promote the apoptosis of cells. HCCR-2 may be involved in cell regulation and the proliferation of hepatocellular carcinoma cells.

When senescence induction is based on DNA damage, senescent cells display a unique phenotype, which has been termed “senescence-associated secretory phenotype”( SASP ) . SASP, including proinflammatory cytokines, growth factors, chemokines, matrix remodeling enzymes and other cytokines, may be an important driver of chronic inflammation and therefore may be part of a vicious cycle of inflammation, DNA damage and senescence. Senescence-associated secretory products released by such cells can affect the neighboring cells and further exacer-bate their regenerative capacity. SASP is associated with many chronic age-related diseases.

Non-coding RNAs ( ncRNA ), including ribosomal RNA( rRNA), transfer RNA( tRNA), MicroRNA ( miRNA), long noncoding RNA(lncRNA) and small nucleolar RNA(snoR-NA), are a class of RNA that have multiple functions and are not translated to proteins. MicroRNA and lncRNA are involved in the injury, remodeling and aging of blood vessels, and it is necessary to understand the regulatory roles of MicroRNA and lncRNA in these processes. It is reported that MicroRNA and lncRNA are not only participated in the regulation of oxidative response, inflammation, cell proliferation and migration, and phenotype transition, they are also involved in the regulation of gene expression by conducting different mechanisms, including transcriptional regulation, post-transcriptional modification and chromatin remodeling. These aspects of regulation by MicroRNA and lncRNA are related to cardiovascular diseases, such as ath-erosclerosis, hypertension, myocardial infarction, stroke, pul-monary hypertension and diabetes, and thus provide a new way for genetic diagnosis and therapy of cardiovascular diseases.