Objective:To explore the influence of psychological and to identify determinants for patients using position immobility by thermoplastic sheet in the course of radiotherapy. Methods:55 patients who received radiotherapy using position immobility by thermoplastic sheet were investigated with questionnaires. Results:There are 34.5%(19/55) patients admit under psychological stress during the treatment, which were affected by the tension from doctors’ consultation and the chemotherapy experience. Conclusion:Some patients were under psychological stress caused by the position immobility with thermoplastic sheet. Nursing intervention is necessary to improve, especially to the patients who show stress when consultation with doctors and who had chemotherapy experience.

Objective To seek the effect of SGK1 on the protection and prognosis of cerebral ischemia reperfusion. Methods Animal model of rats was used to construct them into experimental group(A)and control group(B). The impact of cerebral ischemia reperfusion on the hippocampus neuron cell apoptosis was simulated by making the overexpression of SGK1 and using PI3K inhibitors LY294002 to deal with animal models. Results The overexpression of SGK1 could reduce the neuron cell apoptosis caused by cerebral ischemia reperfusion to a certain extent,reverse the expressions of Cleaved aspas 3 protein,pro-apoptotic protein Bax and inhibitor of apoptoasis protein Bcl-2 through the PI3K/Akt-mediated signaling pathway. Conclusion SGK1 can protect the tissues with ischemia-reperfusion,which may provide the biological evidence for future clinical applications.

Objective To explore the relationship and clinical significance among glycosylated hemoglobin (HbA1c),fasting blood glucose (FPG),insulin (Ins),C peptide (CP)and urinary mirco-albumin (urine m-Alb)inpatients with type 2 diabetes melli-tus (T2DM).Methods A total of inpatients with type 2 diabetes were choose in this study and divided into mild diabetic group (HbA1c ≤ 9%)with 45 cases,severe diabetic group (HbA1c > 9%)with 44 cases.Twenty healthy persons were selected into control group.The levels of HbA1c,FPG,Ins,CP and m-Alb in urine of each group were detected and compared,the relationships of them were analyzed.Results There were significant differences on the levels of HbA1c,FPG,Ins,CP and m-Alb in urine of each group,and the former three indicators from low to high followed by the control group,the mild diabetic group,the severe diabetic group.There was no significant difference on the level of Ins between the control group and the severe diabetic group(P >0.05), but the level of Ins in the mild diabetic group was significant higher than that of the control group and the severe diabetes group (P <0.05).The CP level in the mild diabetic group was significant higher than that in the control group (P <0.05),and has a posi-tive correlation with the level HbA1c (r=0.427,P <0.05 ).The CP level in the severe diabetic group was significant lower than that in the control group,(P <0.05)and was negatively correlated with the level HbA1c (r=-0.415,P <0.05).Conclusion The joint detection of HbA1c,FPG,Ins,CP and urine m-Alb has positive significance to control type 2 diabetes,protect islet function and prevent diabetic nephropathy,CP is better than Ins on the assessment of the secretory function of islet,and CP might be in-volved in the occurrence and development of diabetic nephropathy.

Thirty patients with vitiligo were treated by cupping of skin lesion and compress of Chinese herbs, compared with western medical treatment. After 3-course's treatments the total effective rate ws 96.7% in treatment group and 76.7% in control group. There was a significant difference (P＜0.05)between the two groups shown by statistical analysis.

Objective To investigate the change and clinical value of serum procalcitonin (PCT ) in the patients with severe dia‐betic foot .Methods The serum PCT level was detected before treatment and at 1 ,2 weeks of treatment in 86 patients with severe diabetic foot .Results With the infection severity aggravating before treatment ,the elevation of PCT was more significant .The PCT level after 1 week treatment showed a decreasing trend ,but the difference was not statistically significant (P>0 .05);in the patients with Wagner grade 3-5 ,the PCT level after 2 week treatment showed a obvious decreasing trend ,moreover the difference was sta‐tistically significant(P<0 .05) .Conclusion PCT could serve as one of sensitive indexes to judge the outcome in the patients with diabetic foot .

Objective To explore the effects of health education based on mobile platform for patients after coronary stent implantation. Methods Totally 118 patients underwent coronary stent implantation were recruited using convenience sampling method,and divided into the experimental group and the control group by random number table method with 59 cases in each group. The control group received face-to-face health education during hospitalization from nurses,and were followed up at 1 month,3 months and 6 months through phone or outpatient follow-up. The experimental group received health education based on mobile platform. Self-designed questionnaire was used to investigate health behavior and self-efficacy at 1 month,3 months and 6 months for two groups. Results The scores of health questionnaire in the experimental group were better than those in the control group at 1 month,3 months and 6 months,and the difference was statistically significant (P<0.001);there was no difference in self-efficacy between two groups at 1 month(P>0.05),but at 3 months and 6 months the differences were significant (P<0.001). Conclusion Health education based on mobile health education platform for patients after coronary stent implantation can effectively improve health behavior and self-efficacy,and it is worthy of clinical popularization.

BACKGROUND: Cervical orthosis is one of the non-surgical therapeutic methods for the remedy of cervical deformities. The introduction of cervical orthosis into the clinical remedy of cervical deformities resulted by burn can maximally restore the cervical function of the patients.OBJECTIVE: To analyse the clinical applicative mode and the effects of moulded cervical orthosis (MCO) on cervical burn.DESIGN: A before-and-after verification study by employing pateitns as subjects.SETTING: Prosthetics and orthotic department in a provincial artificial limb center.PARTICIPANTS: Totally 19 cases including 13 males and 6 females after skin-transplantation due to serious burn in head, neck and chest admitted by the outpatient department of prosthetics center between January and September 2003.METHODS: Nineteen patients with cervical burn wore MCO. Return visits were made afterwards for the evaluation of the treatment effects of MCO.MAIN OUTCOME MEASURES: Resutls of therapeutic effectiveness evaluation.RESULTS: All nineteen patients with cervical deformities due to burn reached the effects of correcting cervical flexion and restoring cervical functions through wearing orthosis. The cervical functions restored entirely in seven cases, almost restored in eight cases and improved in four cases.CONCLUSION: MCO can be clinically used on cervical deformities resulted by burn and provide good effects on remedy and rehabilitation.

To discuss the possibility and methods of pathphysiological bilanguage teaching.The 350 juniors were surveyed by self-designed questionnaire about pathphysiological bilanguage teaching issues.Which appear that most students believe the pathphysiological bilanguage teaching is very important and they hope Chinese and English should be combined.It is necessary to carry out the pathphysiological bilanguage teaching at present,and at the same time explore an appropriate method through practical teaching work.

AIM: To observe the changes of glutamate and calcium within the hippocampal microenvironment in mitochondrial stress.METHODS: A lateral hippocampus was microperfused with glutamate and calcium chloride solution by a kind of single-pumped push-pull perfusion system in Tree Shrews.At 24 h,the expression of cytochrome C(Cyt C)was observed by immunochemistry.Also,the hippocampus was removed,then mitochondria and cytoplasmic fragment were divided by low temperature centrifugation and the distribution of cytochrome C was assessed through Western blotting.The relative amounts of caspase-3 and caspase-9 mRNA were evaluated by real time fluorescence polymerase chain reaction.In the treated group,cyclosporin A(CsA,40 mg/kg) was intravascularly injected at 6 h after perfusion of glutamate-calcium chloride solutions into the hippocampus and inspected the above-mentioned items at 24 h.RESULTS: In the glutamate-calcium group,compared with the control group,cytochrome C immunoreactivity increased and the content of hippocampal mitochondrial cytochrome C decreased.Also,the cytochrome C was detected in cytosol.Cyclosporin A treatment at 6 h after microperfusion,the cytochrome C expression weakened and no Cyt C in cytosol fraction was observed.By real time PCR,in relation to the control group,the caspase-3 and caspase-9 mRNA was higher in the glutamate-calcium group.Cyclosporin A treatment cut down both caspase-3 and caspase-9 mRNA contents.CONCLUSION: The accumulation of glutamate and calcium may promote Cyt C release,caspase cascade activation and the mitochondrial stress.The neuroprotection of CsA may results from uniquely inhibiting the mitochondrial permeability transition pore,and preventing Cyt C release and caspase activation.

Objective To investigate the feasibility, indications, operating technique and the clinical value of laparoscopeic surgery for ectopic pregnancy with prior history of obstetric and gynecologic operations. Methods 82 ectopic pregnancy patients with prior history of the obstetric and gynecologic operation were subjected to laparoscopic surgery. Among them, 60 patients had undergone prior obstetric and gynecologic operation only once, 21 patients had undergone twice, and the remaining one had undergone obstetric and gynecologic operation three times. The laparoscopic operation was completed in different ways as follows: puncture and injection of drug under laparoscopy, salpingectomy, resection of pregnancy in oviduct, removal of embryo through incision of oviduct under laparoscope, suction removal of embryo from oviduct, and new technique for pregnant surgery at interstitial portion. Results Of these 82 patients undergoing laparoscopic surgery, 73 cases had adhesion in different degree, and the adhesion rate was 89.0% (73/82). In the 60 patients with history of one operation, pelvic peritoneal adhesion was found in 51 patients, the adhesion rate was 85.0%. All 22 patients with two prior operations had pelvic peritoneal adhesion, and the rate was 100.0%. The laparoscopic surgery was successful in 70 cases. The rate of success was 85.4%, while in 12 patients, the operation was changed into laparectomy due to dense adhesion, and the rate was 14.6%. Of these 12 patients, in 5 of them there was endometriosis, in 6 patients chronic pelvic inflammation was found, 9 patients had a surgical history for ectopic pregnancy, 2 patients with the history of caesarean section, and one patient had a history of complicated gynecologic operation. Complications included colonic injury in one patient with ectopic pregnancy, and the operation was changed to laparotomy. Injury to the ureter happened in one patient. The operation time for laparoscopic surgery was 30-180 min. Conclusions The laparoscopic operation is an effective procedure and it has become a routine procedure for ectopic pregnancy with prior history of obstetric and gynecologic operation. Because of chronic pelvic inflammation and endometriosis, it is difficult to perform laparoscopic surgery successfully, and laparotomy should be anticipated at all time.