1.Recent progresses in endotoxin-induced p38 MAPK signal transduction
Lin YAO ; Shuqin YU ; Xiran ZHANG
Chinese Journal of Pathophysiology 2000;0(12):-
A Review The diseases caused by endotoxin have seriously affected human health. Previous studies have shown that p38 MAPK pathway is involved in the intracellular signal transduction induced by lipopolysaccharide (LPS), which plays an important role in the activation of inflammation-related cells to release inflammation mediator. Recently there have been some progresses in the isoforms distribution, substrate, molecular mechanism of regulating the release of inflammatory mediators, cellular specific activation and levels of p38 MAPK. [
2.Mechanism of inhibitory effects of atropine and anisodamine on contractions of rabbit thoracic aorta
Shuqin LIU ; Weijin ZANG ; Zengli LI ; Qiang SUN ; Xiaojiang YU
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
0.05, respectively), but those to noradrenaline (NA: 0.01?mol/L), histamine (His: 3?mol/L), and 5-hydroxytryptamine (5-HT: 0.1?mol/L), significantly reduced (P0.05, respectively). Under atropine or Ani pretreatment, the NA-, His- and 5-HT-elicited contractions in endothelium-denuded aorta were similar to those in endothelium-intact aorta. Conclusion Atropine and Ani can inhibit receptors-mediated constrictions of rabbit aortic vascular smooth muscle cells; the actions are endothelia independent.
3.Effect of epigallocatechin-3-gallate on LPS-induced p38 MAPK pathway in mouse macrophages
Lin YAO ; Xiran ZHANG ; Shuqin YU ; Kaihe DU
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To investigate the effect of epigallocatechin-3-gallate (EGCG) on lipopolysaccharide (LPS)-induced p38 MAPK activation and tumor necrosis factor-? (TNF-?) secretion in macrophages. METHODS: Western blotting was used to detect the phosphorylation of p38 MAPK in mouse macrophages cultured in vitro. Enzyme linked immunosorbent assay was used to determine the secretion of TNF-? in macrophages. Electron microscopy was used to study the effect of EGCG on the structure of LPS. RESULTS: LPS caused activation of p38 MAPK and more production of TNF-?, EGCG inhibited LPS-induced phosphorylation of p38 MAPK and TNF-? production and had no effect on the structure of LPS. CONCLUSIONS: EGCG has no direct effect on LPS, but blocks cellular signal pathway. The inhibition of EGCG on LPS-induced TNF-? production is mediated, at least in part, through blocking of p38 MAPK pathway. [
4.The effects of rhIL-11 on hematopoietic restoration following cyclophosphamide-induced thrombocytopenia in cynomolgus monkeys
Shuqin YU ; Zhenli ZHANG ; Yong SUN ; Jian TONG
Chinese Journal of Biochemical Pharmaceutics 2001;22(2):61-64
Purpose The aim is to evaluate the effects of rhIL-11 made in China on hematopoietic recovery following chemotherapy-induced thrombocytopenia in cynomolgus monkeys.Methods Chemotherapy-induced myelosuppression of cynomolgus monkeys was made by china iv cyclophosphamide ( 30 mg/kg, qd ) for 5 days, then animals were divided 6 groups(n=4), by sc rhIL-11( 50, 100, 200 μg/kg), or Neumega (GI rhIL-11 control, 100 μg/kg) for 14 days, another normal control and model control. Blood was taken before chemothery and then at day 3, 6,9,12,15,18,21 for blood cell counts and platelet congregate test. Bone marrow was aspirated day 0, 12 and 21 for evaluation of the megakaryocyte ploidy distribution.Results Recovery of blood platelets was accelerated and reached normal levels by day 12, and was higher than normal day 18, day 21 sc rhIL-11 in cynomolgus moneys. Blood platelet congregated rate were 71.4%~74.6% by day 21 and higher than model control . megakaryocytes in bone marrow increased.Conclusion rhIL-11 could accelerate the recovery of peripheral blood platelets in monkeys. The function of increased platelets was normal. The results supported the clinical use of rhIL-11 as a platelet restorative agent to prevent severe thrombocytopenia following chemotherapy.
5.Effects of nesiritide on vascular remodeling and aldosterone in rabbits after angioplasty in arteria iliaca externa
Shuqin LIU ; Zhongzhi LIU ; Hongwen XIE ; Ke YU
Journal of Chinese Physician 2011;13(3):305-307
Objective To reveal the relation between Nesiritide and vascular remodeling,the relation between Nesiritide and the levels of aldosterone.Methods 20 male New Zealand rabbits were randomly divided into two groups.All rabbit models were constructed with BC.After injury,the control group were fed with standard forage,the Nesiritide group received standard forage plus subcutaneous Nesiritide[0.1 mg/dissected free from arteria iliaca externa,processed for histological and morphological study by TD 2000 analysis system of pathology image.Data of vascular remodeling and the levels of plasma aldosterone were determined.Results There had significant difference between AUIEM and AUEEM(P < 0.05),markedly significant difference was also found in residual cavity areas and ratios of stenosis(P <0.01).There had no significant difference between areas of media(P > 0.05).Aldosterone reduced from the 28th day.Conclusions Nesiritide could benefit vascular remodeling after angioplasty,which might be through reducing aldosterone.
6.The application of play therapy in hospitalized children during the course of lumbar puncture operation
Yan HONG ; Jingbo YU ; Peijuan FANG ; Shuqin MIAO
Chinese Journal of Practical Nursing 2015;31(15):1140-1142
Objective To evaluate the impact of play therapy to the pain and the compliance of hospitalized children during tht course of lumbar puncture operation.Methods Divided 60 children with lumbar puncture operation into the intervention group (30 cases) and the control group(30cases)randomly.Routine cares were used in the control group,while the play therapy was used in the intervention group in addition during the course of lumbar puncture operation.Results There were statistical significance in the comparisons of the changes of respiratory rate and pulse rate during the preoperative and intraoperative of lumbar puncture between the two groups,P<0.01.There were also significant differences in the comparisons of subjective and objective pain score as well as the puncture compliance in the lumbar puncture operation.The score of FLAFF scale and the Wong-Baker scale in the intervention group was (2.52 ± 1.37) and (3.59 ± 1.15) respectively,which was significant lower than that of in the control group,(4.22 ± 1.97) and (4.93 ± 1.71),the difference was significant,P<0.01.Conclusion Play therapy can relieve the pain of children with lumbar puncture operation,reduce the changes of the vital signs,promote the puncture compliance and shorten the time of the whole operation completeness.
7.Protective effects of resveratrol on acute lung injury induced by lipopolysaccharide in mices
Shujian XU ; Xingxing HUANG ; Ruifang ZHU ; Shuqin YU
Chinese Journal of Biochemical Pharmaceutics 2009;30(6):375-378,382
Purpose To investigate the effect of resveratrol on acute lung injury induced by lipopolysaccharide (LPS) in mice.Methods Acute lung injury (ALI) was induced by LPS in mice. The changes of lung airway inspiratory resistance (Ri), expiratory resistance (Re), and dynamic lung compliance (Cdyn) were observed with pulmonary function test apparatus. Brochoalveolar lavage fluid (BALF) was collected, and the concentrations of interleukin 1β (IL-1β)、interleukin 6 (IL-6) and tumor necrosis factory α (TNF-α) were measured by ELISA. The ratio of wet to dry weight was calculated to assess lung edema. Pulmonary vascular permeability was examined with injection Evans blue to judge the destructive extent of alveolar epithelial cell and endothelial. Pathological section was made, and the histopathological change was observed with light microscope.Results Resveratrol can inhibit the elevation of Ri and Re, and the descent of Cdyn. Simultaneously, resveratrol reduced the concentration of IL-1β, IL-6 and TNF-α,as well as the wet to dry weight ratio and the pulmonary vascular permeability significantly. Furthermore, it also could attenuate the lung injury on histopathology.Conclusion The results show that pretreatment with resveratrol has a protective effect on ALI induced by LPS. The ultimate inhibiting and release of inflammatory factors were involved in the mechanism of the effects.
8.Laparoscopic choledochocele resection,report of 48 cases
Shuqin WANG ; Qizhi YU ; Jingbo FU ; Long LI
Journal of Peking University(Health Sciences) 2004;0(03):-
Objective: To summarize surgery experience and efficiency of laparoscopic excision of choledochal cyst and reconstruction of biliary tract and to analyze the treatments administered during intra and post-operation of the biliary reconstruction. Methods: We enrolled 48 cases of video-guided laparoscopic intraoperative cholangiography, cholecystectomy, choledochocele resection, Roux-en-Y hepaticojejunostomy with an anti-reflux valve from August, 2001 to October, 2003.Their operative procedures and aims were retrospectively analyzed. Results: Forty-four out of 48 patients successfully underwent laparoscopy and recovered soon. Only 4 cases were transformed to open operation. The post-operation complications were found in 4 cases. Conclusion: Video-guided laparoscopic excision of choledochal cyst, biliary tract reconstruction and Roux-en-Y hepaticojejunostomy are an effective method of treatment with advantages of minimal injury, less bleeding, and sooner recovery.
9.Repair of severe hypospadias with Duckett's and Duplay's technique
Shuqin WANG ; Yu LEI ; Baofu LIU ; Long LI
Chinese Journal of Rehabilitation Theory and Practice 2005;11(9):751-752
ObjectiveTo assess the effect of Duckett and Duplay technique on repairing severe hypospadias.Methods70 cases with severe hypospadias accepted urethraplasty with a penile and scrotal skin tube by Duckett's and Duplay's technique. ResultsThe hypospadias of 65 cases were successfully repaired in one stage, 3 cases complicated urethral fistula,2 caese complicated urethral stenosis with urethral diverticulum, which was significantly improved compared with the scrotal cutaneous island flap techniques(P<0.05). ConclusionDuckett's and Duplay's techniques is a good method in the treatment of severe hypospadias with little complication.
10.Surgical treatment of liver tumor in childhood
Shuqin WANG ; Yu LEI ; Long LI ; Liuming HUANG ; Zheng WEI ; Ping LIU
Chinese Journal of General Surgery 1993;0(01):-
Objective To investigate the diagnosis and treatment of liver tumor in children. Methods Among 23 cases,21 cases underwent resection. Results Benign tumors were in 10 cases and malignant tumors in 11 cases. AH patients were followed up for 0. 5 - 3 years. Two cases died during the follow-up. Three year survival rate in benign tumor was 100% ( 10/10) and 82% (9/11 ) in malignant tumor. Conclusions Most malignant liver tumors were embryonal in children. Combination therapy of surgery and chemotherapy improves the prognosis. Prognosis of benign tumors after total resection is satisfactory.