Objective To observe the effect of leflunomide, combined tripterygium glycosides in treatment of nephrotic syndrome. Methods 64 patients with refractory nephrotic syndrome, according to drug using were divided into two groups,34 patients received the conventional therapy plus leflunomide combined tripterygium glycosides,30patients received conventional therapy alone, such as cyclophosphamide and prednisone,effects and follow-up were observed,as well as kidney function, blood lipid changes. Results After 30d treatment,the efficacy were observed. The results showed the efficiency of the observation group were significantly higher than control group. After 1 year followup,the recurrence rate of the observation group was significantly lower than the control group. 24h urine protein in both groups decreased significantly, ALB was higher than before treatment ,TG TCh was significantly lower than before treatment,and different indexes in the treatment group were significantly better than after the reduction and increase the level of the control group(P ＜ 0.05). Conclusion Lellunomide,tripterygium glycosides combined treatment of ne phrotic syndrome had good effect,and recurrence rate was low,and it could improve kidney function,blood fat,it was worthy of wide dissemination and application.

Objective To design a new portable vital signs monitor for family use. Methods TMS320F2812, a kind of digital signal processor, was applied as the main processor to control the functional mod-ules including A/D convertor, LCD display. Bluetooth communication module was introduced to achieve real-time fast transfer of data between the system and the PC. Results Not only can the system monitor electrocardiogram (ECG), breath rate, pulse rate, body temperature and other vital signs accurately, but also it runs stably. Conclusion The features of DSP and Bluetooth were well combined in the design of the family-oriented, easy to use, multi-functional monitor with small size, low power consumption and convenience. A broad application and market prospects can be predicted.

Objective To observe the efficacy and safety of safflower yellow injection in the treatment of sta-ble angina pectoris.Methods 70 patients with stable angina pectoris were selected and divided into observation group and control group by random number table method,35 cases in each group.The observation group used injection of safflower,and the control group used Xuesaitong.The effect was compared between the two groups.Results The total effective rate of the observation group was 94.3%,which was higher than 74.3% of the control group,the differ-ence was statistically significant(χ2 =5.285,P <0.01).The clinical indexes of the two groups after treatment were significantly improved than before treatment,the differences were statistically significant (t =13.021,25.407,3.376, 3.930,7.119,10.163,18.044,3.765,3.124,6.096,all P <0.05).The angina attack times of the observation group was less than that of the control group ,the dosage of nitroglycerin was lower than that of the control group ,the differences were statistically significant(t =4.686,6.568,all P <0.05).The incidence rate of adverse reaction was 5.7% in the observation group,compared with the control group of 8.6%,the difference was not statistically signifi-cant(χ2 =0.729,P >0.05).Conclusion The injection of safflower yellow pigment in the treatment of stable angina pectoris has high safety.

Myelodysplastic syndromes (MDS) is a heterogeneous group of clone bone marrow disorders.The diagnosis of MDS can be challenging when dysplastic features are minimal and cytogenetic analysis shows no abnormalities The high sensitivity of flow cytometry (FCM) in the detection of dysplasia in myeloid lineages makes it a valuable tool to distinguish possible clonal causes of cytopenia(s) from nonclonal causes.Due to the heterogeneity and absence of specific FCM markers for MDS,the immunophenotyping of MDS by FCM remains a big difficulty.Here we will focus on how to perform immunophenotyping of MDS by FCM and the potential diagnostic,prognostic and therapeutic value of FCM in MDS.

Objective To study the value of laparoscope in the diagnosis and treatment of high cryptorchidism. Methods 21 testes in 19 patients were performed by intraperitoneal exploration and cryptorchidopexy. Results The testes of 11 cases were located in inferior extremity of kidneys and the ones of 8 cases in superior part of internal rings.15 testes were fixed in the bottom of scrotums and 4 ones in abdominal cavity for secondary operation; absent testis was found in 1 case; 1 testis was removed due to testis dysplasia. Scrotum emphysema and hematoma occurred in 3 and 1 patients respectively. Wounds of 19 cases healed well postoperatively. 15 patients had been followed up for (3～10) months with an average of 6 months and they recovered well. Conclusions The diagnosis and treatment of high cryptorchidism by means of laparoscope has the advantages of minimal invasion, less bleeding, quicker recovery and no serious complications.

Aim To study IL-8 expression of U937 cells directly induced by Pseudomonas aeruginosa(PA)and its mechanism through the mitogen-activated protein kinase pathways.Methods The expressions of IL-8 mRNA in human monocytic leukemia cell lines(U937 cells)infected by Pseudomonas aeruginosa which were in differently differentiated and its protein secretion in the supernatant of culture medium were examined by the methods of RT-PCR and ELISA respectively.And these were then to be compared with those inhibited by MAPK inhibitors.Results Pseudomonas aeruginosa was able to induce IL-8 mRNA expression and their protein secretion both in U937 cells and in PMA differentiated U937 cells and had marked time and concentration dependent relations.Pretreament of U937 cells with specific inhibitors of ERK1/2(PD98059),the kinase that activated ERK1/2,p38(SB203580)could significantly diminished the PA-induced IL-8 production(P

Objective To explore the effect of spironolactone on vascular remodeling of injuried rabbit artery. Methods 20 male New Zealand rabbits were randomly divided into two groups. The rabbit models of iliac artery angioplasty were set up by BC. After injury, control group was fed with standard forage, and experimental group was fed with standard forage and spironolactone(20mg?kg~ -1?d~ -1). The rabbits were killed four weeks after injury. The injuried segments of iliac artery were removed, and processed for histological and morphological analysis by imumohistochemistry and TD 2000 analysis system of pathology image. Results AUIEM and AUEEM in the experimental group significantly increased as compared with the control group(P0.05). Conclusion Spironolactone could improve vascular remodeling after angioploasty of iliac artery in rabbits.

Objective To observe the analgesia effects of nitrous oxide inhalation in childbearing. Method Respectively estimate the labor time,delivery type,degrees of labor pain, fetal distress and postpartum hemorrhage condition between analgesia group of 86 cases and control group of 45 cases. Result Compared to control group, the painful extent of delivery process in analgesia group was obvious lightened and childbearing time was decreased. Conclusion BZ Analgesia effects of nitrous oxide inhalation is positive in childbearing and has great value of clinical applying in obstetrics.

Objective:To investigate the expressions of tumor stem cell marker CD133 and tumor vascular endothelial cell marker CD105 in lung cancer tissue and their clinical significance.Methods:Streptavidin-biotin-peroxidase complex (SABC)-immunohistochemical staining was used to detect the expression of CD133 and CD105 in 65 cases of lung cancer tissue and 30 specimens of adjacent non-cancerous tissue. The relationship between the expression of CD133/CD105 and tumor size, histological types, differentiation, TNM stage, lymphoid metastasis, and prognosis were analyzed. Results:The positive expression rates of CD133 and CD105 in lung cancer tissues were significantly higher than those in adjacent non cancerous tissues, respectively (69.2% vs 26.7% and 67.7% vs 10.0%, both P<0.05). The positive expression rates of CD133 and CD105 in the group with lymphoid metastasis were higher than those with non lymphoid metastasis, respectively (82.5% vs 48.0% and 80.0% vs 48.0%, both P<0.05). The expressions of CD133 and CD105 were positively correlated with lymphoid metastasis with Pearson coefficient r of 0.35 and 0.32, respectively (P<0.05). No significant correlation was found between the expression of CD133/CD105 and tumor size, histological types, differentiation degree as well as TNM stage(P>0.05). The expression of CD133 was positively correlated with the expression of CD105 in lung cancer tissue with Pearson coefficient r of 0.37 (P<0.05). Postoperative median survival periods of CD133 and CD105-positive group were significantly shorter than the CD133 and CD105-negative groups (37 months vs 66 months,35 months vs 70 months, respectively, both P<0.05). Conclusion:The expression of CD133 and CD105 was associated with lymphoid metastasis and prognosis in patients with lung cancer.Their overexpression implies poor prognosis of lung cancer patients.

Objective To explore the short-term prognosis of tirofiban hydrochloride on the patients with acute ST-segment elevation myocardial infarction and the influence of tirofiban hydrochloride on myocardial biomarkers. Methods 334 patients with acute ST-segment elevation myocardial infarction were choosen. They were divided into two groups, 167 cases in each group. Tirofiban hydrochloride was used in the experimental group, and conventional therapy was carried out for control group. Compared the biochemical indexes, myocardial biomarkers and cardiac function of the two groups. Results The effective rate of experimental group was 165 cases (98.80%), which was higher than 150 cases (89.82%) in the control group (χ2=12.556,P<0.05). After treatment, the cardiac troponin I (cTnI) in experimental group was lower than control group (P<0.05). The levels of left ventricular end diastolic volume (LVEDV) and left ventricular endsystolic volume (LVESV) significantly decreased and the level of left ventricular ejection fraction (LVEF) increased in experimental group compared with those in control group(P<0.05).The global registry of acute coronary events (GRACE) after treatment in experimental group was lower than that in control group (P<0.05). The hyporrhea of thrombolysis in myocardial infarction (TIMI) in experimental group were 5 cases (2.99%), which was lower than 19 cases (11.38%) in control group (χ2=8.799,P<0.05). After 12 months, the attack frequency of angina in experimental group was (1.78±0.78)times, which was lower than (2.56±1.04) times in control group (P<0.05). Conclusion Patients with acute ST-segment elevation myocardial infarction should be diagnosed as soon as possible and take treatment strategies according to individual situation to chose suitable dosage of tirofiban hydrochloride for good therapeutic effect.