Objective To research the effect of Dingxin capsule on the level of TXB2 and MMP-9 in rats, and explore the mechanism of treating and preventing atrial fibrillation (AF) by TCM. Method AF animal model was established by injecting the calcium chloride-acetylcholine mixed liquid in coccygeal vein of rats. The rats with typical ECG were divided randomly into 4 groups:AF model group (M-group), Dingxin capsule group (D-group), verapamil group (V-group), normal group (N-group). TXB2 and MMP-9 were measured 4 weeks later. Result Comparing with M-group, the level of TXB2 and MMP-9 of D-group decreased obviously (P

Objective To observe the effects of recombinant staphylokinase (r-SAK) on platelet activation parameters in patients with acute myocardial infarction(AMI) by intravenous thrombolysis in order to investigate the clinical thrombolytic efficacy of r-SAK therapy in AMI comparing with recombinant tissue-type plasminogen activator(rt-PA) therapy.Methods Thirty-three patients with AMI within 12 h after the onset were selected and divided randomly into the r-SAK therapy group(n=17) and rt-PA therapy group(n=16).Coronary artery angiography(CAG) was performed 90 min after thrombolytic therapy in patients.Thrombin-antithrombin complex(TAT) and alpha granule membrane protein(GMP-140) were measured by similar commercial enzyme-linked immunosorbent assay(ELISA).Results In r-SAK group and rt-PA group,the plasma contents of GMP-140 2 h after thrombolytic therapy were significantly higher than before therapy(P0.05).In rt-PA group,the plasma content of TAT 2 h after thrombolytic therapy increased significantly(P0.05).) Conclusion r-SAK has similar effect with rt-PA and it will become available for highly fibrin-selective thrombolytic therapy of AMI.Thrombolytic treatment with r-SAK can improve the injury of myocardial microperfusion.

Objective The relationship between XbaI polymorphism of ApoB gene and cerebral infarction(CI)among Chinese population was assessed by Meta-analysis.Methods All related case-control studies were collected from all publications,the relevant studies were identified after eliminating those poor-qualified studies.Meta-analysis was conducted for investigating heterogeneity among individual studies,and summarizing the effects across studies.Results The combined data statistics revealed the frequencies of the X-X+/X+X+ genotypes showed no statistically difference(Z=1.72,P=0.08).Through the subgroup analysis,it was obviously increase the risk of CI in Chinese northern population(OR=2.66,95%CI:1.67~4.24),but no statistically difference in Chinese southern population(OR=1.26,95%CI:0.90~1.77).Conclusions ApoB gene XbaI polymorphism may be significantly associated with susceptibility of CI in Chinese northern population,but not a definite risk for Chinese southern population.

As a rising automatic identification technology, radio frequency identification (RFID) is receiving considerable attention and eliciting widespread interest in medical institutions in China. Some medical institutions have begun exploring and developing RFID systems for kinds of applications. A majority of medical institutions pay insufficient attention to the issue of RFID interference with medical devices. Specialized standards for the application of RFID in healthcare environments are desired. And there are misalignments between existing technology standards. Further researches should be developed to study the influence on safety and efficiency of medical devices induced by RFID system.

Objective To investigate the clinical features and treatment of mycoplasma pneumoniae infection complicated with allergic purpura.Methods 40 children with mycoplasma pneumoniae infection complicated with allergic purpura were selected.Clinical symptoms,disease treatment and outcome were analyzed retrospectively.Results 25 cases were abdominal type allergic purpura,9 cases were articulated joints allergic purpura,6 cases were kidney patients with allergic purpura.In addition,some patients showed upper or lower respiratory tract infections.All the patients received blood circulation improvement,anti-allergy and other conventional treatment,and on the basis of this,azithromycin,low-dose dexamethasone or gamma globulin was given.All 40 patients were cured,the average length of hospital stay was (18.6 ± 3.1) d.Conclusion Mycoplasma pneumoniae infection may trigger allergic purpura purpura-like rash,the clinical symptoms,some accompanied by lower respiratory infections,should give the conventional anti-mycoplasma pneumoniae infection treatment,and the treatment time should be sufficient to cure allergic purpura,shortening hospitalization time,has an important role in the prevention of recurrent disease.

Objective To investigate ASPS induced G_2/M arrest in lung cancer cell line H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis was used to inspect the proteins of ERK, p-ERK. Results Compared with control group, G_2/M phase cells increased with concentration significantly, G_0/G_1 phase cells were not different, G_2/M phase cells and G_0/G_1 phase cells were not different when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, protein of p-ERK was significantly increased, expression of ERK was no different. Conclusion ASPS may induce G_2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.

Objective To observe the effects of ultrasound medium frequency electrotherapy on pain and content of enkephalins. Methods The pain threshold of the rat was measured before and after the application of ultrasound medium frequency electrotherapy. The content of met enhephalin(MEK) and leu enkephalin(LEK) in the glandula hypophysis and hypothalamus was also measured. A linear regression analysis based on the pain threshold and the content of enkaphalin was conducted to reveal the pain relieving mechanism of the ultrasound medium frequency electrotherapy. Results The level of MEK and LEK in the glandula hypophysis and hypothalamus was significantly increased after a single use action ultrasound medium frequency electrotherapy as compared with that before the treatment ( P

ObjectiveTo determine endometrial thickness, endometrial volumeand, endometrial and subendometrial blood flows, by using transvaginal two-dimensional (2D) and three-dimensional (3D) ultrasound examination and to compare the value of all the parameters in the prediction of endometrial receptivity during in vitro fertilization-embryo transfer (IVF-ET) cycles.MethodsOne hundred and twenty patients undergoing IVF cycles were recruited for this study. Transvaginal 2D and 3D ultrasound examination was performed to determine endometrial thickness, endometrial volume, pulsatility index (PI), resisitance index (RI) , systolic/diastolic ratio (S/D) of endometrial, subendometrial blood flows , vascularization index (VI), flow index (FI) and vascularization flow index (VFI) of endometrial and subendometrial regions by experienced sonographers on the day of human chorionic gonadotropin (HCG) injection. Statistical comparison was carried out by Mann-Whitney test. The receiver operator characteristic (ROC) curve analysis was applied and the area under the curve (AUC) was calculated. Statistic analyses were performed using SPSS 13.0. The two-tailed value of was considered statistically significant.ResultsThere were no significant differences between the pregnant group and non-pregnant group in endometrial thickness, endometrial volume (P>0.05). Patients in the pregnant group had lower PI, RI and S/D of endometrial and subendometrial blood flows than those in the non-pregnant group (Z=-3.811,-3.097,-2.071, P=0.001, 0.001, 0.038), with statisticalsignificant difference. Patients in the pregnant group had higher VI, FI and VFI of endometrial and subendometrial regions than those in the non-pregnant group (Z=-1.970,-2.698,-1.981, P=0.490, 0.007, 0.048;Z=-2.098,-2.090,-2.115,P=0.036, 0.037, 0.034), with statistical significant difference. ConclusionsEndometrial and subendometria blood flows measured by transvaginal 2D and 3D ultrasound can be as a reliable indicator to assess endometrial receptivity and predict the pregnancy outcome. Among all the endometrial and subendometrial blood flow parameters, VI of endometrial and subendometrial are better predicators.

Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer (mass fraction :bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ) and a kind of enzyme la-beled antibody stabilizer (mass fraction :Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ) were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .

Objective:To assess the clinical efficacy of two different diameter Osstem MS one-stage implant restoration of small edentu-lous space in the mandibular anterior region.Methods:85 patients were treated by Osstem MS one-stage implant with the diameter of 2.5 mm(n =66)and 3.0 mm(n =66)respectively for the restoration of small edentulous space in mandibular anterior region.The mesi-al and distal marginal bone level and soft tissue were statistically analyzed after 1 2 and 24 months of functional load.The implant survival rate was evaluated according to Wheeler's survival criteria.Results:The survival rate of the implants was 1 00%.The mean changes in marginal bone level(mm)on the mesial side of 2.5 mm and 3.0 mm diameter implants were 0.275 ±0.638 and 0.098 ±0.31 9,distal aspects were 0.360 ±0.588 and 0.1 09 ±0.323 after 1 2 months of functional load;while 0.299 ±0.672 and 0.099 ±0.31 8,0.381 ± 0.581 and 0.1 07 ±0.31 9 after 24 months of functional load.The mesial and distal marginal bone loss of 2.5 mm diameter implant was greater than that of 3.0 mm after 1 2 and 24 months of functional load(P <0.05).No significant change on the marginal bone level was found aomog the same diameter implants from 1 2 to 24 month observation(P >0.05).No relevant complication of peri-implant soft tissue was shown.Conclusion:Favorable clinical effects including function and aesthetics can be achieved by Osstem MS one-stage implant with the diameter of 2.5 mm or 3.0 mm for the restoration of small edentulous space in the mandibular anterior region,however,the mar-ginal bone loss was greater around 2.5 mm diameter implant.