Acanthamoeba castellanii(T4)was cultured with different concentrations of allitridium for 24 hours, and examined by transmission electron microscope. The results showed that the ultrastructure of Acanthamoeba trophozoites was destroyed gently at concentration of 50 ?g/ml allitridium and seriously destroyed under the concentration of 500 ?g/ml, indicating that allitridium is effective in destroying Acanthamoeba.

Objective To investigate the clinical effect of Shaoyao Gancao Decoction (SGD) combined with conventional therapy in treatment of neck cervical spondylosis.Methods 119 cases of neck cervical spondylosis from Jiaxing Hospital of Zhejiang Armed Police Hospital during January 2013 to January 2015 were retrospective analysis, 68 patients were treated with SGD combined with conventional physical therapy (combination group), 51 patients were only adapted conventional physical therapy (physiotherapy group).The clinical efficacy between two groups were compared.Results After treatment, the cervical curvature, neck pain, neck tenderness, cervical flexing function,cervical lateral curvature function,affect the condition of work, the impact of the disease on sleep scores of combination group were significantly lower than physiotherapy group (P<0.05).NPQ and McGill scores of two groups were significantly lower than pre-treatment ( P<0.05 ) , and NPQ, McGill scores of combined group were significantly lower than physiotherapy group (P<0.05).Therapy cure rate of combined group was 32.35%, which higher than 19.61%of physiotherapy group (P<0.05). Conclusion SGD combined with conventional therapy in treatment of neck cervical spondylosis was better than conventional therapy alone.

Objective To explore the risk factors for early interhepatic recurrence and metastasis of hepatocellular carcinoma (HCC)on CT imaging before treatment.Methods 1 1 5 patients suffered from HCC from July 2003 to January 2009 were retrospec-tively enrolled for reviewing their clinical characteristics and CT signs.The status of metastasis and/or recurrence was followed reg-ularly.Signs on pre-treatment enhanced CT images were measured and analyzed.Analysis of variance and independent sampler t test were applied for Univariate survival analysis.Then multivariate analysis was carried out by the Logistic regression,Lon rank meth-od,and p-value < 0.05 was defined to be statistically significant.Results The early interhepatic recurrence and metastasis rate of the study group was 58.26%.With univariate analysis,tumor size,location,extent,capsule,satellite nodule,vascular invasion, AVM and necrosis were the risk factors for early recurrence and metastasis of HCC on pre-treatment enhanced CT imaging (P<0.05).Multi-variable Logistic regression analysis showed that tumor size,satellite nodule,vascular invasion capsule and were independently sig-nificant CT signs for early interhepatic recurrence and metastasis of HCC (P =0.031,0.005、0.037、0.048).Conclusion Pre-treat-ment enhanced CT imaging with HCC was closely related to early interhepatic recurrence and metastasis of the tumor.A tumor of larger size,with satellite nodules,without complete capsule and vascular invasion on CT may predict a tendency to early interhepatic recurrence and metastasis of HCC.

Purpose To compare the CT features of hepatic metastases of gastro-entero-pancreatic adenocarcinomas with and without neuroendocrine differentiation [NED(+) and NED(-)] and to explore the value of CT features in differentiation of the two groups.Material and Methods From January 2009 to December 2015,abdominal CT scans of 17 pathologically proved cases of NED(+) gastro-entero-pancreatic adenocarcinomas with hepatic metastases and 34 pathologically proved cases of NED(-) hepatic metastases with sex,age and primary site matched were retrospectively reviewed.CT features including hepatic metastases number,size,distribution,shape and enhancement were assessed,as well as presence of lymphadenopathy or ascites.Differences of CT features between the two groups were analyzed.Results Compared with NED(-) group,hepatic metastases of NED(+) group more frequently demonstrated a peripheral enhancement on artery phase (94.1％ vs.44.1％,P＜0.05),and more washout on portal venous phase (41.2％ vs.5.9％,P＜0.05),while hepatic metastases of NED(--) group showed more plateau type (91.2％ vs.58.8％).There was no significant difference of other findings between the two groups (P＞0.05).Logistic regression revealed that enhancement area in hepatic artery phase and enhancement changes in portal venous phase were independent factors for differential diagnosis (P＜0.05).The area under the ROC curve of combining the two features was 0.811 (P＜0.05).Conclusion There are some different CT enhancement features between NED(+) and NED(-) hepatic metastases of gastro-entero-pancreatic adenocarcinomas,which are helpful in differential diagnosis.