Object As stated in the subject. Methods By field cultivation.Results In sexual reproduction, (1) short tree branches can be used instead of the conventional tree trunks. Sow four layers of seed on to two timber layers. The peretration rate of Amillaria mellea (Vahl. ex Fr.) Karst into the germinated Gastrodia elata Bl.protocorm can attain well over 50%. After half year, the yield of both small and medium sized gastrodia tubers (2) Sow four layers of seed onto every two layers of long tree trunks may double the yield. In asexual propagation, the use of short tree branches not only can save timber material but also maintain the yield.Conclusion This new cultivation method can be used to save timber while maintaining the quality and quantity in the production of G. elata.

The changes of active oxygen species and some enzymes in Grifola umbellata induced by Armillaria mellea elicitor were studied.The results showed that active oxygen species appeared in both mycelia and sclerotia of G.umbellata after treated with A.mellea.There were two phases of active oxygen production upon addition of A.mellea elicitor.Phase I occured at 10 minute after addition of A.mellea elicitor.Phase Ⅱ occurred about 90 minute.The changes of some enzyme activity were also studied in this paper.Compared with control,the A.mellea elicitor could reduce the activity of superoxide dismutase and peroxidase.The catalase activity changed only little.The phenylanine ammonia lyase activity declined in the early stages and then increased in the late stages.

Object To study the promoting effects of Mycena anoectochila, M dendrobii and M. orchidicola on growth of Dendrobium candidum Wall. ex Ldl. and Anoectochilus roxburghii (Wall ) Ldl Methods The protocorm and plantlet of D candidum and A roxburghii were grown in dual culture with endophytic fungi to observe the proliferation of protocorm and the growth of plantlet. Results The growth of plantlet of D candidium, which was inoculated three endophytic fungi, increased markedly 3 5 times more than those of the control one M dendrobii and M orchidicola also promoted multiplication on protocorm of D candidum (P

Object Effectively extracting RNA from Anoectochilus roxburghii (Wall.) Ldl. lays a foundation to research the mechanism of endomycorrhiza promoting its growth. Methods RNA was prepared by CTAB, electrophoresis on 1% agroses gel and photo are taken with MutiImage TM Light Cabinet. Results The ratios of RNA A 260 /A 230 and A 260 /A 280 were 2.054 and 1.818, respectively. And the yield was 154.35 ?g/g fresh. The bands were clear on agrose gel and integrity of RNA was good. Conclusion RNA with high quality and high quantity is isolated by the method of CTAB for medicinal plants with high concentrations of polysaccharides and phenolics.

Process for the preparation of artificial seeds of Dendrobium candidum was established by using the somatic embryos of this plant. Experimental result showed that 1/2MS medium supplemented with sucrose was suitable to induce germination and seedling of D. candidum artificial seeds. The germination rate was over 80%.

Objective To isolate and identify the chemical constituents in the polar part of the Ancecotochilus roxburghii.Methods The flavonoids were isolated by column chromatography and their structures were elucidated through spectral analyses such as FAB-MS and NMR.Results Five flavonoids were isolated and identified as quercetin 7-O-?-D-glucoside(Ⅰ),quercetin 3-O-?-D-rutinoside(Ⅱ),isorhamnetin-3,4′-O-?-D-diglucoside(Ⅲ),isorhamnetin-3,7-O-?-D-diglucoside(Ⅳ),and isorhamnetin-7-O-?-D-diglucoside(Ⅴ).Conclusion All the compounds are obtained from A.roxburghii for the first time.

Objective To study the effects of two fungal elicitors on the growth of Dendrobium candidum protocorms cultured on the solid medium.Methods The medium for propagation of protocorms was selected and the growth curve on that medium was obtained.According to the curve,the two fungal elicitors were added at the different growth stages of protocorms.The fresh weight(FW) and dry weight(DW) of protocorms were measured.Results The medium for propagation of protocorms was 1/2MS+20% potato extract+3% sucrose+0.8% agar.Compared with the control MF23 elicitor added at weeks 11 and 13 could significantly increase the FW by 16.4%(P