Objective To investigate the different compatibility of tripterygium wilfordii on its attenuation and synergistic effects through applying Leigongteng decoction and its demolition decoctions on nephrosis rats,and to select the optimizing decoction to treat nephrotic syndrome.Methods 110 Wister rats were divided into 11 groups randomly,which were 9 groups of nephrosis model groups (including Leigongteng group,Leihe group,Leigui group,Leiqi group,Leigongteng ecoction group,Leijin group,Leigan group,prednisone group and nephrosis model group) and 2 blank groups (normal control group and normal Leigongteng group),10 rats in each group.The normal control group and normal Leigongteng group was intravenous injected with 0.65 ml sodium chloride in caudal vein; 9 groups of nephrosis model groups were injected with 0.65 ml adriamycin.The prednisone group was selected for positive control,24h urine protein,total protein,albumin,urea nitrogen,and creatinine were tested with automatic biochemistric meter.Results ALB (27.58±2.62)g/L,Leigan group Compared with(92.60± 18.12) mg in Leigongteng group,the urine protein in Leigongteng diction group(48.60± 11.75) mg,Leiqi group (53.20± 13.21) mg,and Leigan group (56.40 ±16.52) mg were reduced.Compared with the Leigongteng group TPR(58.98±4.84) g/L,ALB (20.98 ± 3.49) g/L,the Leigongteng decoction group [TPR (68.47 ± 5.25) g/L,ALB (28.97± 3.01) g/L],TPR and ALB in Lei-Qi group TPR(68.21 ±4.19) g/L,TPR(67.51 ± 5.42) g/L,ALB (26.56± 1.89) g/L were all elevated.Compared with Leigongteng group chol (1.48 ± 0.65) mmol/L TG (1.70 ± 1.58) mmol/L,chol and TG in the Leigongteng decoction group chol(0.78±0.13)mmol/L,TG(0.56±0.14)mmol/L,Lei-Qi group chol (0.70±0.13) mmol/L,TG (0.65±0.12) mmol/L,and Leigan group chol (0.97±0.33) mmol/L,TG(0.66±0.18) mmol/L were lowered; compared with Leigongteng group,UN (14.20 ± 2.12) mmol/L,Cr (78.10 ± 6.14) μmol/L,UN and Cr in the Leigongteng decoction group UN(7.59±0.78) mmol/L,Cr(39.60±8.33) μmol/L,Leigan group UN (9.48 ± 0.82) mmol/L,Cr(38.30±7.89)μmol/L,and Leijin group UN(9.71±1.33) mmol/L,Cr(41.40±6.45) μmol/L,Leigui group UN(8.30±1.12) mmol/L,Cr(36.90±6.15) μmol/L were all reduced with significant differences (all P＜0.05).The changes of Kidney morphology and ultrastructure pathology in Leigongteng different combination groups were lighter than the control groups.Conclusion Leigongteng decoction group,Leiqi group,Leigan group had synergistic actions,while Leigongteng decoction group,Leigan group,Leijin group,and Leigui group had kidney toxicity-reducing actions.

Objective To study the effects of treating herpes zoster with the combination of Chinese and western medicine.Methods 160 cases of herpes zoster were randomly recruited into a treatment group(n=80),and a control group (n=80).The control group was treated with westem medicine(acyclovir,ethacridine solvents,and vitamin B6 and B12).The treatment group was administrated with Chinese medicines,acupuncture and cupping on the basis of treats in the control group Results The total effective rate was 100%in the treatment group and 72.5%in the control group.There was significant difference between the two groups(χ~2=23.85,P＜0.05).Conclusion The combination of Chinese and western medicine is effective in treating herpes zoster and worthy of generalization.

Objective To study the topical application of Tuhuai decoction and its disassembled prescriptions on skin function of mice with epidermal hyperplasia, and to provide a theoretical basis for the treatment of psoriasis. Methods According to the Denda method, skin barrier function in mice was destructed with tape, to produce epidermal hyperplasia model. Tuhuai decoction and its disassembled prescriptions with different concentrations of water and alcohol extracts were external applied on the mice. Since the beginning of the treatment, loss of skin moisture of both sides the trunk was detected with Multi-functional instrument at every day before using the medicine. Results Tuhuai decoction had function of reducing the amount of water loss of skin. The disassembled prescriptions, such as Angelica group, safflower group, Danshen root group, and water and ethanol extracts of Prtmella group can reduce the amount of water loss of skin effect. Conclusion Water and ethanol extracts of Tuhuai decoction has the functions of reducing water loss of skin and resisting hyperplasia,suitable for treating psoriasis by external application, setting a foundation for clinical treatment of psoriasis.

Objective:To observe the effect of internal and external use of Chinese material medica with syndrome differentiation on acne vulgaris.Methods:200 patients with acne vulgaris were randomly divided into treatment group(110 cases)and control group(90 cases).Treatment group were given acne facial mask,oral medicine decoction,and the control group were treated with13-cis-Retinoic Acid Capsules,topical Clindamycin Gel,7 weeks for a course of treatment,the clinical effect were compared.Results:The effect on facial lesion in treatment group and control group had significant difference(P

Objective: To investigate the expression and role of LINC00052 during glycidyl methacrylate (GMA) -induced malignant transformation of 16HBE cells. Methods: Human bronchial epithelial (16HBE) cells were divided into GMA transformation group and corresponding DMSO control group, and the 10th, 20th and 30th generation cells of each group were collected LncRNA microarrays were used to analysis expression of LINC00052 in different stage of malignant transformation. Bioinformatics analysis was applied and the relative expression of LINC00052 and its potentially target genes was detected by real-time quantification PCR (qPCR) . Results: The results of microarray analysis showed that LINC00052 was up-regulated by 1.32-fold, down-regulated by 1.64-fold and down-regulated by 4.92-fold in the malignant transformation early (P10) , middle term (P20) and late (P30) , respectively, The results of qPCR showed that compared with the DMSO control group, the expression of LINC00052 was up-regulated by 1.55 times, down-regulated by 1.20 times and down-regulated by 2.35 times in P10, P20 and P30, respectively, and the difference was statistically significant (P<0.05) . There was a statistically significant difference in the relative expression of NTRK3 between the GMA transformation group of P10 and P30 generations with the corresponding DMSO control group (P<0.05) . Conclusion LINC00052 is highly expressed in early time of GMA-induced malignant transformation of 16HBE, and down-regulated in the middle and last stage of malignant transformation and may play a protective role in GMA-induced malignant transformation of 16HBE by influencing the expression of its target gene NTRK3.