Using observation of the gross and histological changes of skin, intestine, liver and spleen, the spleen index, the popliteal lymph node weight gaining, and the ~(125)I-UdRincorporation assay, the effects of GvHR in the pathogenesis of immunologically mediatedaplastic anemia in mice were studied. Rtsults showed that there were no gross and histological changes due to GvHR foundin skin, intestine, liver and spleen, and the spleen index decreased markedly, and therewere positive popliteal lymph node weight gaining and positive ~(125)I-UdR incorporationassay which showed a dose dependent corrlation. These results suggested that, transfusion of DBA/2 lymphocytes into lethally irradia-ted BALB/c mice could evoke GvHR, but on the other hand, the effects of GvHR werenot very important in the pathogenesis of immunologically mediated aplastic anemia inmice.

Multiglycosidorum Triptergii are widely used in China. We treated 37potients who had suffered from child asthma for 12 weeks with this drug (1.0～1.5mg/kgday) and observed for about 8 months. The results showed that 33 of them were curedor relieved. The effective rate was 89.18%. In order to study its immune mechanism, the percentage of T subsets and T supperessive rate were measured for 27 patients.Theresults showed that CD3~3 and CD4~4 subpopulation had no change,but the percentage ofCD8~+ increased from 25.36?5.16% to 28.96?3.03%,the ratio of CD4~+/CD8~+ declined(P

Only in recent years, attentions have been drawn to the significance of expressing killer cell inhibitory receptors (KIR) in T cells KIRs specifically bind to the corresponding region of the MHC class I molecules and transmit negative signals to prevent cytotoxity of T cells. When the ligands of KIRs are missing, the lysis of the target cells can't be avoided. Perhaps the existence of KIRs is the main mechanism for preventing T cells from attacking autologous tissues. The recognition mechanism of the interaction between the KIR + donor T cells and the recipient's MHC class I molecule expressing tissue cells might shed light on the establishment of the immunotolerance for the prevention of allo-graft rejection and graft-versus-host disease.

Bone marrow stroma provides the microenvironment for hematopoiesis and is also the source of mesenchymal stem cells. Marrow mesenchymal stem cells are capable of self-renewal and differentiation into all mesodermal cell types and neuro-ectodermal cells, such as osteoblast, chondrocyte, myoblast, T/L fibroblast, stromal cell, adipocytes, neuron, astrocytes, and so on. These abilities make the mesenchymal stem cells as an excellent target cell of tissue engineering, cell transplantation and gene therapy.

In this research, we apply the antisense strategy to study the function of IGF- I in human hepatoma lines(7402) by Lipofectin mediated gene transfer. The effect of antisense IGF-I on the expression of MHC and the sensitivity of LAK cytotoxicity of tumor cell were studied. The results showed that human hepotoma lines(7402) were transfected with an amplifiable antisense IGF- I gene. The expression antisense IGF-I RNA in the transfected cells after hygromycin resistant selection was confirmed by northern blot analysis. The transfectants expressed higher level of MHC class I and class II molecules. The susceptibility of the transfected cell to LAK cytotoxicity was greater than that of 7402 parent cells(P

Mesenchymal stem cells (MSCs) are a population of multipotent cells that can proliferate and differentiate into marrow and non-marrow cell types, such as adipocytes, chondrocytes, myocytes, and so on. In recent years, many researchers have studied whether MSCs are capable of differentiation into neurons in vivo and ex vivo. The result that MSCs-derived neurons express NSE and NF, but don't express GFAP suggests MSCs can differentiate into neurons, some researchers have achieved success in promoting functional recovery in Pakinsons and transactional spinal cord injury rat models by use of MSCs-derived neurons. Therefore, MSCs-derived neurons will play an important role in the therapy for a variety of diseases of the nervous system. [

AIM: The purpose of this study was to establish a new strategy for constructing the mouse ? 2m gene targeting vector in order to increase the homologous recombination frequency in contrast with our previous one, which was successfully constructed in the normal way.METHODS: A 4.2 kb 3' arm and a 0.8 kb 5' arm were amplified by PCR from the mouse ? 2m-pSV2△HXgpt genomic clone. They included the start region and the three exons, which were separated into two parts from exons 2 (the main coding block) for the two arms——5' arm and 3' arm.RESULTS: The two fragments, in reverse orientation to the Neo gene, were cloned into pPNT respectively on either side of Neo. They were identified by PCR, restriction analysis and sequence analysis as well. CONCLUSION: The mouse ? 2m gene targenting vector has been cloned successfully.

A Review] CD4+ T cells can be divided into Th1/Th2 subsets. Th1/Th2 imbalance participates many disease processes. A stable surface marker distinguishing Th1 and Th2 will greatly facilitate the investigation of Th1/Th2 interaction.Several surface molecules have been reported to be differentialy expressed between Th1 and Th2 cells.LAG-3,active ligands for P- and E-selectin ,IL-18R, IL-12R?2,CC chemokine receptor (CCR5) were shown to be dominantly expressed on Th1 cells,whereas expression of CD30,ST2L,CRTH2,CCR3,CCR4 was reported to be preferential to Th2 cells. In this review, several surface molecules were mainly discussed.

AIM: To investigate the expression of nitric oxide synthase (NOS) and its clinical significance in hepatic cellular carcinoma (HCC). METHODS: The NOS1, NOS2 and NOS3 of 51 cases of HCC and 46 cases of liver tissue beside carcinoma (LTBC) were detected by immunohistochemistry. RESULTS: The expressive rates of NOS1, NOS2 and NOS3 in LTBC were significantly higher than those in HCC ( P

AIM: To investigate the effect of cytomegalovirus (CMV) on the expression of ICAM-1 and VCAM-1 in bone marrow stromal cells and on the adhesion of bone marrow stromal cells to hematopoietic cells. METHODS: Flow cytometry was used to detect the expression of adhesion molecules ICAM-1 and VCAM-1 in bone marrow stromal cells, MTT method was used to perform the adhesion assay of bone marrow stromal cells to normal hematopoietic cells. RESULTS: Bone marrow stromal cells could be infected by the CMV used in this experiment; CMV below the dose of 100TCID 50 could not destroy bone marrow stromal cells apparently; The expression of ICAM-1 increased at the early stage(18 h) of CMV infection, the expression of ICAM-1 decreased at the late stage (120 h) of CMV infection. Inactived CMV could also increase the expression of ICAM-1 as alive CMV; The adhesion rate of bone marrow stromal cells to hematopoietic cells increased at the early stage of CMV infection. CMV had no significant effects on the expression of VCAM-1 in bone marrow stromal cells. CONCLUSION: The adhesion capacity of bone marrow stromal cells to hematopoietic cells increased at the early stage of CMV infection, while the adhesion capacity of bone marrow stromal cells to hematopoietic cells decreased at the late stage of CMV infection.