1.A retrospective study comparing endoscopic self-expandable metallic stents with surgery in the treatment of malignant obstructive jaundice
Ying SHI ; Xiaoxing CHEN ; Shunfu XU ; Wenfang CHENG ; Hong ZHU
Chinese Journal of Hepatobiliary Surgery 2012;18(2):118-122
Objective To compare the efficacy and survival of patients with malignant obstructive jaundice using either endoscopic self-expandable metallic stents or surgery,and to evaluate the compounding factors influencing prognosis.Methods 56 patients with malignant obstructive jaundice treated with endoscopic self-expandable metallic stents (the endoscopic group) were compared with 90 patients who received surgery (the surgery group) during the same study period.Clinical data and survival of the 2 groups of patients were retrospectively analyzed.Results The success rate was 100% in the endoscopic group.The serum bilirubin,alkaline phosphatase (ALP) and γ-glutamyl transferase (γ-GT) decreased significantly by using either therapeutic endoscopy or surgery (P<0.01).There was no significant difference between the two groups in the reduction of serum total bilirubin.The mean survival of the endoscopic and surgery groups were 340 d and 795 d respectively.The accumulative survivals of the endoscopic group at 3,6 and 12 months as evaluated by the Kaplan-Meier method were 82.6 %,61.1% and 46.6 %,respectively,and for the surgery group were 97.0%,90.9 % and 65.4% respectively. There was a significant difference in survival between the two groups (P<0.01).Survival after therapeutic endoscopy was similar to surgery for patients with metastasis and hilar biliary obstruction.Conclusions Self-expandable metallic stents gave similar palliation in the relief of jaundice in patients with malignant biliary obstruction.The stents had no effect on the primary tumor.Therapeutic endoscopy with self-expandable metallic stents is a safe and effective method for the relief of jaundice in patients with obstructive jaundice caused by non-resectable malignant tumors.
2.Regulatory Effect of Rhodiola Crenulata on Senescence of Human Embryo Lung Fibroblasts Diploid Cells Through PI3K/AKT-HDAC2 Axis
XU Zhilian ; YANG Huiqing ; HONG Shunfu ; RUAN Fei
Chinese Journal of Modern Applied Pharmacy 2023;40(18):2512-2518
OBJECTIVE To explore the regulatory effect and mechanism of salipurposide(Sali), salidroside(Sal) and isoflavone(Isota) from Rhodiola crenulata on senescence of human embryo lung fibroblast diploid cells(2BS). METHODS Observing and selecting young 28 generations 2BS(28PD) cells and senescence 50 generations 2BS(50PD) cells under the microscope. MTT method was used to determine the effects of 1.25, 2.5, 5.0, 7.5, 10.0 μmol·L-1 Sal, 2.5, 5.0, 7.5, 10.0, 12.5 μmol·L-1 Sali and Isota on the activity of 50PD cells. The 5.0 μmol·L-1 Sal, 10.0 μmol·L-1 Sali and Isota were used for the following experiments according to MTT results. 28PD and 50PD cells were divided into 28PD group, 50PD group, 50PD+Sali group, 50PD+Sal group, 50PD+Isota group, intervention for 24 h. The senescence associated-beta-galactosidae(SA-β-gal); malondialdehyde(MDA), reactive oxygen species(ROS), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX) levels of cells were measured with the test kits; the mRNA expression of phosphatidylinositol-3-kinase(PI3K), protein kinase B(AKT), histone deacetylase 2(HDAC2) and the protein expression of PI3K, p-AKT, HDAC2 were detected by qRT-PCR and Western blotting respectively. RESULTS Compared with the 28PD group, 50PD group cell SA-β-gal expression was increased, the content of MDA and ROS were increased, the activity of SOD and GSH-PX were decreased, the mRNA expression of PI3K, AKT, HDAC2 and related protein expression were decreased(P<0.01). 50PD cells after intervention with Sali, Sal, and Isota, SA-β-gal expression were decreased(P<0.01), the content of MDA and ROS were decreased significantly(P<0.01), the activity of SOD and GSH-PX were increased(P<0.01), the mRNA expression of PI3K, AKT, HDAC2 and related protein expression were significantly increased(P<0.05 or P<0.01). CONCLUSION Sali, Sal and Isota from Rhodiola crenulata can reduce the expression of SA-β-gal of 50PD cells, its effect may related to the inhibition of oxidative stress and regulation of expression of PI3K/AKT-HDAC2 axis.
3. Preliminary study on the correlation between diversity of biliary flora and recurrence of common bile duct stones
Xiufang ZHA ; Shunfu XU ; Hong ZHU ; Xiaoxing CHEN ; Wenfang CHENG ; Bin XIAO ; Guosheng CHEN ; Jinliang NI
Chinese Journal of Digestion 2019;39(12):850-854
Objective:
To investigate the correlation between the diversity of biliary bacterial flora and the recurrence of common bile duct stones.
Methods:
From September 2018 to March 2019, 48 patients underwent endoscopic retrograde cholangiopancreatography (ERCP) at the First Affiliated Hospital of Nanjing Medical University were enrolled and divided into primary choledocholithiasis (primary group, 38 patients) and recurrent choledocholithiasis group (recurrent group, 10 patients). The bile of the patients of the two groups was collected and analyzed with high-throughput sequencing of 16S rRNA. The Chao1 estimator indicated the richness of the biliary bacterial flora, and Shannon index and Simpson index demonstrated the diversity of biliary bacterial flora. The bacterial flora distribution was explored from different levels of phylum, class, order, family, genus and species. Chi-square test and