OBJECTIVE To investigate flora distribution and drug resistance status of nosocomial infections with Pseudomonas aeruginosa (PAE) in intensive care unit (ICU) and to provide the scientific reference for clinically reasonable use of antibiotics. METHODS PAE was isolated,cultured and identified according to the National Clinical Laboratory Operation Rules. The antibiotic susceptibility test was performed by K-B method and the results were read according to CLSI 2004-2006. RESULTS Among 254 PAE strains,the isolating rate in the lower respiratory tract samples was the highest (rriving 59.4%). Then the isolating rate in the wound and soft tissue infection samples was 13.8%. The drug-resistance supervision in vitro accounted for 17.3% and 19.7%,respectively,of PAE were resistant to imipenem and meropenem in ICU. The drug resistance rate to aztreonam,trimethoprim-sulfamethoxazole,ciprofloxacin,levofloxacin,gentamicin,cefotaxime,cefepime and piperacillin was all over 60.0%. CONCLUSIONS PAE in ICU is the mostly multi-resistant strain. We should strengthen its monitoring and controlling.

Acute Disease ; Adult ; Female ; Humans ; Male ; Middle Aged ; Tin Compounds ; poisoning

OBJECTIVE To approach the constituent ratio and drug-resistance of Gram-negative bacilli(GNB) in nosocomial infections and provide the scientific evidence for the clinical treatment of infectious diseases.METHODS Totally 376 GNB strains isolated from our hospital were cultured and identified according to the National Clinical Laboratory Operation Rules.The antibiotic susceptibility test was performed by K-B method and the constituent ratio of GNB was analyzed statistically.RESULTS The pathogens with strains having higher isolating rate were Pseudomonas aeruginosa(21.5%),Klebsiella pneumoniae(17.6%),Acinetobacter baumannii(14.4%),Escherichia coli(10.9%),and Stenotrophomonas maltophilia(5.9%).The drug sensitivity tests in vitro showed that these strains were multiresistant.Except for natural drug-resistant S.maltophilia,16.0% of P.aeruginosa and 24.1% of A.baumannii were resistant to imipenem.The average detection rate of the extended spectrum ?-lactamase(ELBLs) producers was 40.2%.CONCLUSIONS The resistance status of GNB is very serious.We must strengthen monitoring and controlling of drug resistance.

Objective To investigate the effect of biofeedback electrical stimulation and pelvic floor muscle training on the rehabilitation of pelvic floor muscle after delivery by vaginal delivery.Methods From January 2014 to June 2015,90 cases who received pelvic floor rehabilitation treatment after vaginal delivery in Department of Gynecology and Obstetrics of our hospital were selected as the research subjects.According to whether the patients received pelvic floor rehabilitation training,they were divided into the treatment group and control group,90 cases in each group.The treatment group received biofeedback electrical stimulation,pelvic floor muscle training methods of rehabilitation at 6 weeks postpartum.The control group did not take any measures of rehabilitation.Results 4 months postpartum,the bladder neck mobility,urethral rotation angle of the treatment group were significantly lower than those of the control group (t =6.052,4.400,all P ＜0.05).the vaginal muscles voltage measured values of the treatment group was significantly higher than that of the control group (t =9.524,P ＜ 0.05).4 months postpartum,in the treatment group,vaginal muscle Ⅰ grade in 24.44％,grade Ⅱ in 55.56％ and grade Ⅲ in 17.78％,grade ⅣV in 2.22％;in the control group,the vaginal muscle strength grade Ⅰ in 44.44％,grade Ⅱ in 55.56％;The vaginal muscle distribution in the treatment group was significantly better than the control group (Z =-2.865,P ＜ 0.05).Conclusion After vaginal delivery,maternal postpartum receiving biofeedback electrical stimulation,pelvic floor muscle exercise can significantly promote the recovery of pelvic floor muscle function.

OBJECTIVE To investigate the distribution and drug resistance of pathogenic bacteria from lower respiratory tract infection in elderly patients.METHODS A total of 1739 strains of bacteria isolated from sputum and lower respiration secretion culture of the elderly patients were identified and their drug resistance was tested by K-B method.RESULTS From 1739 strains of pathogens isolated,Gram-negative bacilli accounted for 63.8%.The main Gram-negative bacilli were Escherichia coli and Pseudomonas aeruginosa,Acinetobacter baumannii,Enterobacter cloacae and Klebsiella pneumoniae.Gram-positive cocci accounted for 22.9% and Candida albicans accounted for 13.3%,respectively.The resistance rates of Gram-negative bailli to imipenem,cefoperazone/sulbactam and amikacine were relatively low.And all Gram-positive cocci were sensitive to vancomycin.CONCLUSIONS The antimicrobial resistance of pathogens in elderly patients with lower respiratory tract infection is serious.It is important to mornitor the antimicrobial resistance.

OBJECTIVE To investigate the antibiotic resistance status in coagulase negative staphylococcus (CNS) strains and provide the scientific evidence for the reasonable use of antibiotics. METHODS The drug resistance of 251 strains of CNS was ananlyzed. RESULTS The detection rate of methicillin-susceptible coagulase-negative Staphylococcus(MSCNS) was 12.75% (32/251), while that of methicillin-resistant coagulase-negative Staphylococcus (MRCNS) was 87.25%. The drug-resistance rate of MRCNS was higher than that of MSCNS. Vancomycin resistant and intermediate strains had not been detected. CONCLUSIONS Laboratory should monitor the drug resistance well and update in time to offer the evidence for the reasonable use age of the antibiotics.

This article sorted out research results of traditional Chinese herbal medicine at home and abroad, cleared up confirmed traditional Chinese herbal medicine with estrogen activity, analyzed the function classifications, and reviewed relevant clinical research progress. It also discussed the concept and connoation of traditional Chinese herbal medicine with estrogen activity, and proposed that the research on traditional Chinese herbal medicine with estrogen activity should clarify whether “bidirectional effect” establishes and the preconditions and requirements of the establishment, with a purpose to solve the security problems about hormone dependency caused by TCM therapy. Meanwhile, the problems about quick-screening experiments, standard establishment, and the transformation of experimental achievements to clinical application are waiting to be solved.

Objective To discuss the changes of the quality of the fresh frozen plasma,which leaved refrigerator and it was out of the cold chain.Method The fresh frozen plasma was placed at the room temperature,in six different periods,that is 0,0.5,2,4 h,8,and 12 hours.The prothrombin time,activated partial thrombin live enzymes time,fibrinogen and factor FⅤ,factor FⅧ,antithrombin(AT) and protein C were tested on each point.Results In the fourth group and the fifth group,the FⅧ,AT,and protein C were statistically lower compared with the controls (P＜0.05).But there were no obvious changes of APTT,PT,INR,Fbg and FⅤwhen placed at room temperature for 12 hours.Conclusion After the fresh frozen plasma placed at room temperature for more than 8 hours,the FⅧ ∶ C,AT and protein C were significantly lower compared with the control group.But they have no obvious changes of APTT,PT,INR,Fbg and F Ⅴ when placed at room temperature for 12 hours.

Recent progress in molecular biology has enabled us to better understand the molecular mechanism underlying pathogenesis of human malignancy including lung cancer, the advent of therapies based on mechanisms that target critical molecular pathways of tumors has evoked considerable interest. The therapeutic index is high, and optimally effective treatment can be achieved at a dose below the maximal tolerated dose,giving us a better understanding of the human lung cancer. This review focuses on the advance of targeted therapy for the first-line, second-line treatment or the maintenance treatment of non-small cell lung cancer.

Lung cancer is the most common cancer and the leading cause of cancer-related deaths in the world. More than 50 % of advanced NSCLC are diagnosed in patients older than age 65 years. Aging is inextricably associated with decreases in marrow reserve, drug clearance, and lean body mass. Elderly cancer patients often present with medical and physiologic challenges that make the selection of their optimal treatment daunting. This article reviews the progress in diagnosis and treatment of lung cancer in the elderly in recent years.