OBJECTIVE To approach the constituent ratio and drug-resistance of Gram-negative bacilli(GNB) in nosocomial infections and provide the scientific evidence for the clinical treatment of infectious diseases.METHODS Totally 376 GNB strains isolated from our hospital were cultured and identified according to the National Clinical Laboratory Operation Rules.The antibiotic susceptibility test was performed by K-B method and the constituent ratio of GNB was analyzed statistically.RESULTS The pathogens with strains having higher isolating rate were Pseudomonas aeruginosa(21.5%),Klebsiella pneumoniae(17.6%),Acinetobacter baumannii(14.4%),Escherichia coli(10.9%),and Stenotrophomonas maltophilia(5.9%).The drug sensitivity tests in vitro showed that these strains were multiresistant.Except for natural drug-resistant S.maltophilia,16.0% of P.aeruginosa and 24.1% of A.baumannii were resistant to imipenem.The average detection rate of the extended spectrum ?-lactamase(ELBLs) producers was 40.2%.CONCLUSIONS The resistance status of GNB is very serious.We must strengthen monitoring and controlling of drug resistance.

OBJECTIVE To investigate flora distribution and drug resistance status of nosocomial infections with Pseudomonas aeruginosa (PAE) in intensive care unit (ICU) and to provide the scientific reference for clinically reasonable use of antibiotics. METHODS PAE was isolated,cultured and identified according to the National Clinical Laboratory Operation Rules. The antibiotic susceptibility test was performed by K-B method and the results were read according to CLSI 2004-2006. RESULTS Among 254 PAE strains,the isolating rate in the lower respiratory tract samples was the highest (rriving 59.4%). Then the isolating rate in the wound and soft tissue infection samples was 13.8%. The drug-resistance supervision in vitro accounted for 17.3% and 19.7%,respectively,of PAE were resistant to imipenem and meropenem in ICU. The drug resistance rate to aztreonam,trimethoprim-sulfamethoxazole,ciprofloxacin,levofloxacin,gentamicin,cefotaxime,cefepime and piperacillin was all over 60.0%. CONCLUSIONS PAE in ICU is the mostly multi-resistant strain. We should strengthen its monitoring and controlling.

Acute Disease ; Adult ; Female ; Humans ; Male ; Middle Aged ; Tin Compounds ; poisoning

Objective To investigate the effect of biofeedback electrical stimulation and pelvic floor muscle training on the rehabilitation of pelvic floor muscle after delivery by vaginal delivery.Methods From January 2014 to June 2015,90 cases who received pelvic floor rehabilitation treatment after vaginal delivery in Department of Gynecology and Obstetrics of our hospital were selected as the research subjects.According to whether the patients received pelvic floor rehabilitation training,they were divided into the treatment group and control group,90 cases in each group.The treatment group received biofeedback electrical stimulation,pelvic floor muscle training methods of rehabilitation at 6 weeks postpartum.The control group did not take any measures of rehabilitation.Results 4 months postpartum,the bladder neck mobility,urethral rotation angle of the treatment group were significantly lower than those of the control group (t =6.052,4.400,all P ＜0.05).the vaginal muscles voltage measured values of the treatment group was significantly higher than that of the control group (t =9.524,P ＜ 0.05).4 months postpartum,in the treatment group,vaginal muscle Ⅰ grade in 24.44％,grade Ⅱ in 55.56％ and grade Ⅲ in 17.78％,grade ⅣV in 2.22％;in the control group,the vaginal muscle strength grade Ⅰ in 44.44％,grade Ⅱ in 55.56％;The vaginal muscle distribution in the treatment group was significantly better than the control group (Z =-2.865,P ＜ 0.05).Conclusion After vaginal delivery,maternal postpartum receiving biofeedback electrical stimulation,pelvic floor muscle exercise can significantly promote the recovery of pelvic floor muscle function.

Bone Transplantation ; Cervical Vertebrae ; surgery ; Humans ; Postoperative Complications ; diagnosis ; etiology ; surgery ; Pseudarthrosis ; diagnosis ; etiology ; surgery ; Reoperation ; Spinal Fusion

The effects of different concentrations of NaF on the proliferation and differentiation of osteroblasts were observed. Osteroblasts were obtained from the calvaria of 24-h newborn Sprague Dawley (SD) rat by using enzymic degestion method and cultured in the medium containing 10-7 mol/L to 10-5( 10-4 mol/L NaF. The rate of cell proliferation, ALP activity and osterocalcin were examined by the methods of MTT, biochemical assay and radioimmunologic assay (RIA) respectively. The results showed that the effect of NaF on the proliferation of osteroblasts was regulated in a double-phase manner: low doses of NaF (10-7 mol/L, 10-6 mol/L,10 5 mol/L) increased cell proliferation by 11. 3 o, 10. 9 % and 10. 6 % (P＜0. 05) respectively, vice versa at high doses (10-4 mol/L, 5×10-4 mol/L). As compared with control group, high doses of NaF decreased the proliferation rate by 9. 6 %, 9. 7 % respectively. The response of cell differentiation to NaF was varied. The influence of NaF on ALP activity was similar to that of cell proliferation. However, all concentrations of NaF used stimulated the secretion of osterocalcin with the highest secreting rate being up to 67. 14 % at 5×10 4 mol/L NaF. It was concluded that the influence of NaF on the proliferation of rat calvarial osteroblasts regulated in a double-phase manner, but the action on cell differentiation was varied.

Objective To evaluate the efficacy of CO-2 laser treatment for patients with precancerous laryngeal lesions.Methods Ninety-four patients with precancerous laryngeal lesions were treated by endoscopic CO-2 laser operation from 2001 to 2006.87 males and 7 females patients had ages between 18 and 85.Results For a follow up ranging from 6 month to 5 years,the outcomes of 81 patients(81/94,86.2%) were successful only through one operation while 10 cases(10/94,10.6%)received more than two operations.The lesions for 3 patients(3/94,3.2%) were found to have turned out malignant at a later stage.Conclusion The carbon dioxide laser has proved valuable in treating patients with precancerous laryngeal lesions.

This article sorted out research results of traditional Chinese herbal medicine at home and abroad, cleared up confirmed traditional Chinese herbal medicine with estrogen activity, analyzed the function classifications, and reviewed relevant clinical research progress. It also discussed the concept and connoation of traditional Chinese herbal medicine with estrogen activity, and proposed that the research on traditional Chinese herbal medicine with estrogen activity should clarify whether “bidirectional effect” establishes and the preconditions and requirements of the establishment, with a purpose to solve the security problems about hormone dependency caused by TCM therapy. Meanwhile, the problems about quick-screening experiments, standard establishment, and the transformation of experimental achievements to clinical application are waiting to be solved.

Objective To discuss the changes of the quality of the fresh frozen plasma,which leaved refrigerator and it was out of the cold chain.Method The fresh frozen plasma was placed at the room temperature,in six different periods,that is 0,0.5,2,4 h,8,and 12 hours.The prothrombin time,activated partial thrombin live enzymes time,fibrinogen and factor FⅤ,factor FⅧ,antithrombin(AT) and protein C were tested on each point.Results In the fourth group and the fifth group,the FⅧ,AT,and protein C were statistically lower compared with the controls (P＜0.05).But there were no obvious changes of APTT,PT,INR,Fbg and FⅤwhen placed at room temperature for 12 hours.Conclusion After the fresh frozen plasma placed at room temperature for more than 8 hours,the FⅧ ∶ C,AT and protein C were significantly lower compared with the control group.But they have no obvious changes of APTT,PT,INR,Fbg and F Ⅴ when placed at room temperature for 12 hours.

OBJECTIVE To investigate the distribution and drug resistance of pathogenic bacteria from lower respiratory tract infection in elderly patients.METHODS A total of 1739 strains of bacteria isolated from sputum and lower respiration secretion culture of the elderly patients were identified and their drug resistance was tested by K-B method.RESULTS From 1739 strains of pathogens isolated,Gram-negative bacilli accounted for 63.8%.The main Gram-negative bacilli were Escherichia coli and Pseudomonas aeruginosa,Acinetobacter baumannii,Enterobacter cloacae and Klebsiella pneumoniae.Gram-positive cocci accounted for 22.9% and Candida albicans accounted for 13.3%,respectively.The resistance rates of Gram-negative bailli to imipenem,cefoperazone/sulbactam and amikacine were relatively low.And all Gram-positive cocci were sensitive to vancomycin.CONCLUSIONS The antimicrobial resistance of pathogens in elderly patients with lower respiratory tract infection is serious.It is important to mornitor the antimicrobial resistance.