Objective To discuss the changes of the quality of the fresh frozen plasma,which leaved refrigerator and it was out of the cold chain.Method The fresh frozen plasma was placed at the room temperature,in six different periods,that is 0,0.5,2,4 h,8,and 12 hours.The prothrombin time,activated partial thrombin live enzymes time,fibrinogen and factor FⅤ,factor FⅧ,antithrombin(AT) and protein C were tested on each point.Results In the fourth group and the fifth group,the FⅧ,AT,and protein C were statistically lower compared with the controls (P＜0.05).But there were no obvious changes of APTT,PT,INR,Fbg and FⅤwhen placed at room temperature for 12 hours.Conclusion After the fresh frozen plasma placed at room temperature for more than 8 hours,the FⅧ ∶ C,AT and protein C were significantly lower compared with the control group.But they have no obvious changes of APTT,PT,INR,Fbg and F Ⅴ when placed at room temperature for 12 hours.

OBJECTIVE: To investigate the characteristics and influence factors of sensitization hy allergens in patients with allergic rhinitis in the junction of Guangdong and Guangxi provinces. METHOD: One thousand one hundred and twenty-four patients who were diagnosed with allergic rhinitis hy clinical history and symptoms underwent a skin prick test. The percent of allergens with positive skin response and Multiple Allergens Positive Scores were recorded, and their influence factors, included age, gender and test period were evaluated. RESULT: The percent of top three inhalant allergens with positive skin tests were as follow: dermatophagoides pteronyssinus (77.0%) ,dermatophagoides farina (77.1%), blattella germanica (42.3%); the percent of top three ingested allergens with loositive skintests were as follow: crab ( 39.5%), shrimp (31.1%), ribbonfish (10.8%). The average Multiple Allergens Positive Score was (3.6 +/- 2.7). The percent of allergens with positive skin response and Multiple Allergens Positive Score of different allergens changed as age, gender and text period changed, and the changing trend of them was accordant. The percent inhalant allergens with positive skin tests in dermatophagoides pteronyssinus, dermatophagoides farinae and cat fur in different gender showed a significant difference. CONCLUSION Acarine allergens were the most common allergens in patients with allergic rhinitis in the junction of Guangdong and Guangxi provinces. The percent of allergens with positive skin response and the severity of multiple allergy were influenced by age, gender and text period. The severity of multiple allergy can be measured by Multiple Allergens Positive Score.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Allergens ; analysis ; immunology ; Child ; Child, Preschool ; China ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; diagnosis ; immunology ; Skin Tests ; Young Adult

As a rising medical science,evidence-based medicine should be extensively used.Applying the principle of evidence-based medicine to the clinical teaching of burns has positive significance for improving teaching quality,which is helpful to the cultivation of students'clinical thinking abilities.

Drug resistance is the main restriction factor for clinical usage of taxanes.This review concentrates on drug-resistance mechanisms of taxanes that include drug target change,avidity change between drug-target interaction,decrease of cellular effective drug concentration,downstream cellular responses to a drug target molecular lesion,and cell cycle regulation-mediated drug resistance.

Adult ; Female ; Health Knowledge, Attitudes, Practice ; Health Services Needs and Demand ; Humans ; Male ; Middle Aged ; Occupational Health ; Steel

Child ; Humans ; Lung Diseases, Interstitial ; diagnosis

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Injections, Intravenous ; Male ; Middle Aged ; Motor Neuron Disease ; drug therapy ; Phytotherapy ; Treatment Outcome

Objective　To study the relationship between atherosclerotic plaques in carotid artery and ischemic cerebrovascular diseases. Methods　The extracranial carotid arteries (ECA) and middle cerebral artery (MCA) of 54 patients with transient ischemic attack (TIA) or cerebral infarction (CI) were examined with doppler ultrasound. The distribution of atherosclerotic plaque, degree of stenosis and ultrasounic classification of ECA and the mean velocity of blood flow in MCA were examined. Results　①Stenosis over middle-grade on asymptomatic side in extracranial internal carotid artery (EICA) in group of patients with TIA was significantly higher than symptomatic side(P<0.01). Stenosis over high-grade on asymptomatic side in ELCA in group of patients with CI was significantly higher than symptomatic side (P<0.01). ②Flat and soft plaque are most common in group of patients with TIA or CI, then are hard and ulcerative plaques. Incidence of soft plaques on asymptomatic side in group of patients with TIA or CI are significantly higher than symptomatic side (P<0.01); ③Among the group of patients with CI, mean velocity of MCA decreased on asymptomatic side in 31 cases (68.9%), and significantly higher than symptomatic side (P<0.01). Conclusion　Atheroclerotic plaques in carotid artery and intracranial hemodynamic characteristics are the important risk factors for ischemic cerebrovascular diseases. These findings have important values in predicting subsequent TIA or CI in asymptomatic subjects.

Until now, more than seventeen parvovirus have been reported which can infect mammals and poultries. The infected cells appeared different properties of apoptosis and death, present a typical cytopathic effect. NS1 is a major nonstructural protein of parvovirus, with a conservative structure and function, which plays an important role in the viral life cycle. In addition to the influence on viral replication, the NS1 also participates in apoptosis induced by viruses. Parvovirus induced apoptosis which is mainly mediated by mitochondrial pathway, this review summarized the latest research progresses of parvovirus induced apoptosis.
Animals ; Apoptosis ; Humans ; Parvoviridae Infections ; physiopathology ; veterinary ; virology ; Parvovirus ; genetics ; metabolism ; Viral Nonstructural Proteins ; genetics ; metabolism

Objective To study the effects of new triple therapy with clarithromycin plus amoxicillin and omeperazole combined into 3 therapeutic therapies, and 3 therapeutic courses to treat children with helicobacter pyloric(Hp) infection.Methods Two hundred and four patients who were diagnosed by gastroscopy as Hp-related gastroentestinal diseases and divided into 3 groups,randomly.Group A was treated with amoxicillin 50 mg/(kg?d)+bismuth citrate 7-8 mg/(kg?d)+metronidzole 15-20 mg/(kg?d) for six weeks;group B took the same drugs but for two weeks;group C was treated with clarithromycin 15 mg/(kg?d)+omeperazole 0.8 mg/(kg?d)+amoxicillin 50 mg/(kg?d) for 2 weeks.Results The rates of eradicate of Hp:group A 73.4%,group B 75%,group C 92%.The differences between group B and group C were very significant.Conclusion Triple therapy is more effective, less duration,well tolerated, less resistant,with higher rate of eradication.