1.Molecular cloning and sequence analysis of(S)-N-methylcoclaurine-3'-hydroxylase gene in Coptis chinensis
Jiayu ZHOU ; Shuming PENG ; Ningfei LEI ; Hai LIAO ; Fang CHEN
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective To clone and sequence the cDNA encoding(S)-N-methylcoclaurine-3'-hydroxylase from Coptis chinensis.Methods The cDNA,encoding(S)-N-methylcoclaurine-3'-hydroxylase,was amplified by RT-PCR with cDNA library of tender leaf as the template.Results The full-length cDNA of(S)-N-methylcoclaurine-3'-hydroxylase(named as CYP80B3) had 1 680 bp with an open reading frame encoding 488 amino acids of protein.The CYP80B3 had 95%,82%,70%,and 68% amino acid sequence homology to the sequence of(S)-N-methylcoclaurine-3'-hydroxylase from C.japonica,Thalictrum flavum,Eschscholzia californica and Papaver somniferum,respectively.The sequence was reported to the GenBank and coded as EF492879.Comparison of sequence with(S)-N-methylcoclaurine-3'-hydroxylase from C.japonica showed CYP80B3 possessed the same functional regions involved with 3'-hydroxylation of(S)-N-methylcoclaurine.Conclusion The cDNA encoding CYP80B3 from C.chinensis was cloned and reported.This work underlays the first step for exploring the pathway of benzylisoquinoline alkaloid biosynthesis and for improving the content of benzylisoquinoline alkaloid in C.chinensis.
2.Tricarbocyclic core formation of tyrosine-decahydrofluorenes implies a three-enzyme cascade with XenF-mediated sigmatropic rearrangement as a prerequisite.
Zhiguo LIU ; Wei LI ; Peng ZHANG ; Jie FAN ; Fangbo ZHANG ; Caixia WANG ; Shuming LI ; Yi SUN ; Shilin CHEN ; Wenbing YIN
Acta Pharmaceutica Sinica B 2021;11(11):3655-3664
Tyrosine-decahydrofluorene derivatives feature a fused [6.5.6] tricarbocyclic core and a 13-membered
3.Correction to: Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants.
Lei YANG ; Xiaohui ZHANG ; Liren WANG ; Shuming YIN ; Biyun ZHU ; Ling XIE ; Qiuhui DUAN ; Huiqiong HU ; Rui ZHENG ; Yu WEI ; Liangyue PENG ; Honghui HAN ; Jiqin ZHANG ; Wenjuan QIU ; Hongquan GENG ; Stefan SIWKO ; Xueli ZHANG ; Mingyao LIU ; Dali LI
Protein & Cell 2019;10(9):700-700
In the original publication the grant number is incorrectly published. The correct grant number should be read as "17140901600". The corrected contents are provided in this correction article. This work was partially supported by grants from the National Natural Science Foundation of China (Nos. 81670470 and 81600149), a grant from the Shanghai Municipal Commission for Science and Technology (17140901600, 18411953500 and 15JC1400201) and a grant from National Key Research and Development Program (2016YFC0905100).
4.Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants.
Lei YANG ; Xiaohui ZHANG ; Liren WANG ; Shuming YIN ; Biyun ZHU ; Ling XIE ; Qiuhui DUAN ; Huiqiong HU ; Rui ZHENG ; Yu WEI ; Liangyue PENG ; Honghui HAN ; Jiqin ZHANG ; Wenjuan QIU ; Hongquan GENG ; Stefan SIWKO ; Xueli ZHANG ; Mingyao LIU ; Dali LI
Protein & Cell 2018;9(9):814-819
Result Analysis
Print
Save
E-mail