Objective To study the expressions of matrix metalloproteinase-3 (MMP-3) and osteopontin (OPN) in cervical carcinoma and their relationship with invasion and metastasis. Methods The expression of MMP-3 and OPN were detected in 54 cases of cervical cancer tissues by immunohistochemical SP method. Compared with 15 cases of normal cervical tissues, the relationship was analyzed between both protein expressions and clinical pathology. Results The expression positive rates of MMP-3 and OPN in cervical cancer tissues (70.37 % and 66.7 %, respectively) were significantly higher than those of normal cervical tissues (20 % and 0, respectively), were significantly higher with infiltration depth >1/2 and lymph node metastasis than those with depth≤1/2 and without metastasis , respectively(P <0.05). The expressions of MMP-3 and OPN were related with depth of cervical infiltration and lymph node metastasis of cervical cancer.Expressions between MMP-3 and OPN were significantly positively correlation(r =0.401, P<0.05). Conclusion The expressions of MMP-3 and OPN are correlated with invasion and metastasis of cervical carcinoma.

Objective]Summary of Professor Liu Hongqi in treating the spleen kidney two deficiency, deficiency of Qi and blood type, kidney deficiency and blood type of threatened abortion experience. [Method]Reading a lot of literatures, combined with the clinical common disease type, respectively, from the basic pathogenesis, basic treatment, and motion etc. summarize professor of treatment of this disease, and 1 case was attached.[Result]Professor Liu Hongqi believes that the clinical syndromes of this disease were spleen and kidney two empty, deficiency of Qi and blood and blood deficiency of the kidney, the incidence of kidney and spleen, closely related. Professor summed up the kidney and spleen during the long clinical course, Yiqi Yangxue Bushen tocolysis and clearing heat, cooling blood to stop bleeding method, were used on prescription Ma ginseng tocolysis particles, the Liangdi Antai Granuales added and subtracting tocolysis, collaborative western medicine, with emotion, achieved a significant effect. Case accurate, fully reflected the clinical experience of Professor Liu Hongqi in the treatment of threatened abortion. [Conclusion]Professor Liu Hongqi's superb skills, rigorous prescription testimonies, have unique insights for the treatment of this disease, the successful experience is worthy of clinical promotion.

Objective To evaluate the consistency of global acne grading system (GAGS) and acne lesion counting. Methods After training 3 dermatologists evaluated 60 acne patients by GAGS and skin lesion counting respectively in randomized order sequentially, and evaluated again 5 days later. Results The kappa-value of GAGS (consistency) was 0.675 among 3 different dermatologists, and 0.847 for the same desmatologist. The kappa-value among the 3 different dermatologists and the same dermatologist for the total lesion count was 0.426 and 0.413 respectively; for pustule count was 0.446 and 0.407 respectively. The kappa-value of total number of the inflammatory lesion counting was 0.429 for the 3 different dermatologists and the kappa-value was

Objective To investigate the expression of Survivin gene and the relationship between Survivin and genesis and development of cervical cancer. Methods The expression of Survivin gene in tissues of 40 patients with cervical cancer, 38 patients with cervical hyperplasia and 10 cases of normal cervical were detected by immunohistochemical SP method, and the relationships between Survivin and the clinical pathological factors were analyzed. Results The expression positive rate of Survivin in cervical cancer tissues (60 %) was significantly higher than that in cervical hyperplasia and normal cervical tissues (42.1 % and 0, respectively), and their differences were statistically significant (P < 0.05). The positive expression rate of Survivin was related with histological grade and the clinical stages of cervical cancer.Conclusion Survivin is related to the genesis and development of cervical cancer.

Toll-like receptors ( TLRs) are pattern recognition receptors, which exist in both cell membrane and cytoplasm, and participate in inflammatory reactions. Some studies have shown that TLR2/4-NF-κB signal pathways mediated by TLR2 and TLR4 could regulate the production of inflammatory factor IL-1β, which played an important role in the attack of gouty arthritis. The article focused on the pathogenesis of gouty arthritis, discussed the structure and distribution of TLRs, the ligands and its mediated signal pathways, the validity in the treatment of gouty arthritis using the signal pathways as the target points and the relationship between the signal pathways and gouty arthritis, and reviewed the research progress in Chinese medicines using the signal pathways as the guidance.

Objective This study was designed to investigate the relationship between programmed cell death 5 (PDCD5) and endometrial carcinoma .Methods 60 tissue samples of endometrial carcinomas ,40 normal proliferative endometrium tissues and 28 endometrial atypical hyperplasia tissues were obtained from patients who underwent operation.The expression of PDCD5 was detected by Real-time PCR and immunohistochemical analysis . Results In normal proliferative endometrium , atypical hyperplasia tissues and endometrial carcinoma , the PDCD5 mRNA was gradually decreased[(1.21 ±0.16),(0.83 ±0.07),(0.42 ±0.02),F=5.637,P<0.05];The posi-tive rate of protein was gradually decreased (82.5%,64.3%,45.0%,χ2 =27.663,P<0.05);The expression of PDCD5 was correlated with TNM stage , histological grade , depth of myometrial invasion and lymphatic metastasis (χ2 =20.875,11.915,9.409,11.148,all P<0.05),but was not correlated with ages (χ2 =0.287,P>0.05). Conclusion PDCD5 might become a potential molecular marker of early diagnosis of endometrial carcinoma .

Objective To detection the carrying situation of Clostridium difficile toxin gene A/B in stool specimens of the pa-tients with ulcerative colitis (UC) and persons undergoing physical examination and to research the relationship between Clostridium difficile toxin gene carrying and UC.Methods The stool specimens were collected from 53 cases of UC(32 cases of active stage and 21 cases of resting stage) and 45 persons undergoing physical examination.Total DNA was extracted from stool specimens.The Clostridium difficile toxin gene cdtA and cdtB were detected by real-time PCR ,then the PCR products were amplified and performed the agarose gel electrophoresis and gene sequencing for conducting the amplification products verification.Results 7 cases of cdtA and 4 cases of cdtB were checked out in the UC group ,in which 5 cases of cdtA and 2 cases of cdtB were in the UC active stage group ,2 cases of cdtA and 2 cases of cdtB were in the UC resting stage group.In the healthy control group ,5 cases of cdtA and 2 cases of cdtB were checked out.The detection rate of cdtA and cdtB had no statistically significant difference between the UC group and healthy control group(P>0.05).There was no statistically significant difference between UC active group and inactive group (P>0.05).Conclusion There is no appearant correlation between the carrying situation of cdtA and cdtB in stool with UC onset and UC stage.

Objective To study the effects of Semen Cassiae extract on lipid metabolism of hyperlipidemia model mice.Methods In this experiment,mice were sc injected with two different concentration of Semen Cassiae extract,and sacrificed at 1,2,and 4 h.The blood samples were withdrawn from the eye vein.The plasma biochemical indexes were detected by using the kit,and real time PCR was used to analyze the expression of adipogenesis genes with time series.Results For hyperlipidemia model mice treated with Semen Cassiae extract serum total cholesterol(TC),triglyeride(TG),and low density lipoprotein cholesterol(LDL-C) could be decreased significantly(P

OBJECTIVE:To observe the compatibility of gatifloxacin for injection with cefradine for injection in 5% glucose injection and in 0.9% sodium chloride injection respectively.METHODS:At room temperature for8hours the change in appearance and pH value were observed,while the content was determined by UV spectrophotometry as well.RESULTS:There was no evident change in appearance after the mixture,but the pH value and concentration changed significantly.CONCLUSIONS:There exists incompatibility between gatifloxacin for injection and cefradine for injection.

OBJECTIVE:To study the compatible stability of gatifloxacin for injection with metronidazole disodium phos-phate in sodium chloride injection.METHODS:The contents of2constituents of the mixed solution stored at25℃within8hours was determined by dual ultraviolet wavelength spectrophotometry,and the appearance,pH value of the mixture were compared with those before.RESULTS:There was no significant change in the content of gatifloxacin after mixing,while the content of metronidazole disodium phosphate has a slight reduction,no significant changes were noted otherwise.CONCLUSI_ ON:Gatifloxacin for injection is compatible with metronidazole disodium phosphate in sodium chloride injection if at25℃within4hours'mixing.