Objective: To screen angiogenesis inhibitor from 26 drugs for promoting blood circulation and removing blood stasis through chick chorioallantoic membrane(CAM)model in vivo.Methods: The experimental groups were 26 drugs for promoting blood circulation and removing blood stasis.Tablets,capsules,pills and powder of the drugs were adopted in the form of saturated concentration,decoction was in the form of concentration of 2g/ml,and injection,oral liquid and electuary were in their own concentration.7-day-old fertilized white chicken eggs incubated at 37℃ in chamber were prepared by creating a window in the shell.A sponge carrier was placed on CAM and treated with different liquid medicine with the dosage of 20?l per egg daily for 3 days.On day 10,the number of CAM blood vessel embranchment 5mm around the carrier was counted with a stereomicroscope.Results: 15 drugs for promoting blood circulation and removing blood stasis obviously inhibited the CAM blood vessels(P

OBJECTIVE:To compare the efficacy and safety of Pingxiao capsule and Shendan Sanjie capsule combined with CP program in the treatment of non-small cell lung cancer (NSCLC) in stage Ⅳ. METHODS:132 NSCLC patients in stage Ⅳwere randomly divided into CP group,CP+Pingxiao group and CP+Shendan Sanjie group. CP group was treated with CP program;based on it,CP+Pingxiao group was orally treated with 6 Pingxiao capsules,3 times a day;CP+Shendan Sanjie group was orally treated with 6 Shendan Sanjie capsules,3 times a day. 21 d was a treatment period,and the efficacy was evaluated after 2 treat-ment periods,improvement of life quality,progression-free survival,1-year survival rate and toxicity reactions were observed. RE-SULTS:The recent effective rate,disease control rate,total improvement rate of life quality,progression-free survival and 1-year survival rate in CP+Pingxiao group and CP+Shendan Sanjie group were significantly higher than CP group,incidences of leukope-nia,thrombocytopenia,gastrointestinal tract and decreased hemoglobin were significantly lower than CP group,the differences were statistically significant(P<0.05);however,there was no significant difference between CP+Pingxiao group and CP+Shendan Sanjie group(P>0.05). CONCLUSIONS:Both Pingxiao capsule and Shendan Sanjie capsule can be combined with CP program in the treatment of NSCLC in stageⅣ,with good safety.

OBJECTIVE: To optimize the macroporous absorbing resin which were of best action in adsorption and desorption on the active components in Naomaitong granules. METHODS: UV spectrophotometry and HPLC was employed to determine the adsorbability and desorption capacity of different macroporous absorbing resins on total anthraquinones, total ginsenosides, total alkaloids and Puerarin. RESULTS: There were differences in adsorption and desorption capacity on active components in Naomaitong granules among different macroporous absorbing resins. Considering the general adsorbability and desorption capacity of different macroporous absorbing resins, AB-8 turned out to be of the best purification effect on Naomaitong granules. CONCLUSION: The results serve as a theoretical basis for the production of Naomaitong granules.

OBJECTIVE: To study extraction method of anthraquinones components of Radix et Rhizoma Rhei and optimize extraction technology.METHODS: Ultrasonic extraction,heating reflux extraction and soxhlet extraction were adopted to extract Radix et Rhizoma Rhei respectively.3 kinds of extraction technology were compared with the content of total anthraquinone,aloe-emodin,rhein,emodin,chrysophanol,physcione as index.The extraction technologies of UV and 5 kinds of anthraquinone compounds were optimized by uniform design method on the basis of previous study.RESULTS: Heating reflux extraction which was optimal extraction technology was as follows: extracting time of 90 min,extracting for 1 time,methanol concentrations of 95%,medicinal mesh of 2.000 7.Comprehensive score of anthraquinones could reach 6.556.CONCLUSION: The extraction technology is reasonable,available in quality control.

Objective To study the effects of Pioglitazone on TLR4 expression and peritoneal macrophage foam cells formation induced by ox - LDL.Methods Foam cells were cultured equally divied into four groups in random:the normal control,pioglizaone group,ox-LDL group and ox-LDL +pioglizaone group.The SD rats peritoneal macrophages were pretreated with ox - LDL(50 mg/mL)and Pioglitazone(20 mmo1 /L)respectively for 24 h.Then they were stained by oil red O to determine foam cells formation.The effects of Pioglitazone on foam cells TLR4 ex-pressions were detected by immunohistochemistry.Results The SD rats peritoneal macrophages of control group and Pioglitazone group are not almost stained by oil red O,but the macrophages of ox-LDL group become dark red after stained by oil red O,and the cells color of ox-LDL + Pioglitazone group is thinner than that of ox-LDL group.The expressions of TLR4 in ox-LDL group are higher than those in control and Pioglitazone group significantly(24 h,t =3.015,t =4.007;48 h,t =3.100,t =4.008,P <0.01).while expressions in ox-LDL +Pioglitazone group are lower than ox-LDL group respectively(24 h,t =4.075;48 h,t =4.150,P <0.05).Conclusion Pioglitazone might inhibit the formation of foam cells by suppressing the expressions of TLR4 in macrophages.

OBJECTIVE:To establish the method for the content determination of total saponins in Naomaitong.METHODS:Colormetric method was applied using panoxadiol as control.The detection wavelength was set at 556 nm.RESULTS:The linear range of panoxadiol was 2.03～10.15 ?g?mL-1(r=0.999 6) with an average recovery of 100.69%(RSD=1.76%,n=6).CONCLUSION:The method is easy to operate with high precision,accuracy and good reproducibility for the quality control of Naomaitong.

Objective To determine the content icariin in the extract of Gushuling. Methods HPLC method was performed on Luna C18 column (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of acetonitrile- water (30∶70) and the UV detective wavelength was set at 270 nm. Results The curve of icariin was linear within 0.416～2.496 ?g, Y=2.0?106X-53 810 (r =0.999 9). Conclusion HPLC method is simple, rapid and accurate, and it is suitable for ananlysis of icariin content in extract of Gushuling .

Objective To study a rapid and sensitive method for determination of virulence-associated genes in O1El Tor,O139,non-O1/non-O139Vibrio cholerae strains.Methods Five pairs of primers were designed respectively ac-cording to cholera toxin sub-unit A gene(ctxA),accessory cholera enterotoxin gene(ace),zonula occludens toxin gene(zot),toxin coregulated pilus A gene(tcpA)and toxR regulatory protein gene(toxR).Multiplex PCR(MPCR)procedures for simultaneously detecting those five genes were established.The gene information of the virulence-associated genes in the Vibrio cholerae strains was obtained through agar gel electrophoresis for products of single amplification of the MPCR.Results The five virulence-associated genes in the positive control Vibrio cholerae O139(MO45strain)could be detected and the results were correct,which could meet the designed request for the method.In the other tested strains(O1EL Tor,O139,non-O1/non-O139)could be detected1to5kinds of the virulence-associated genes.Based on the results of the variety of carried virulence-associated genes,the tested Vibrio cholerae strains could be classified as5kinds of genetypes,and the Vibrio cholerae could be distinguished between toxic and non-toxic strains.The sensitivity of the MPCR approach reached to10 2 cfu/ml.Conclusion This method is rapid,specific and sensitive,which possess great value for practical application.

Objective To characterize the human cell mediated immune response to Treponema pallidum(TP), and to determine the effect of TP infection on the various lymphocyte subpopulations and natural killer cells in the peripheral blood of the patients. Methods Twenty four untreated patients with early syphilis were analyzed,including 3 groups of 8 patients with disseminated syphilides,palmoplantar syphilides and latent syphilis each. Monoclonal antibodies of CD labeled with either fluorescein isothiocyanate or phycoerythrin were used on a flow cytometry to detect the changing of peripheral blood lymphocyte immunophenotypes. Results The results showed that the percentages of panT,T H,NK cells and the ratio of T H/T S were significantly lower (P0.05). Conclusion It suggests that there is prominent suppression of cellular immunity in the untreated patients suffering from early syphilis. Meanwhile, the difference of 3 groups in skin lesions was due to the different dermatobarrier function against the systemic invasion of TP, rather than attributed to the alteration of the lymphocyte immunophenotypes in the peripheral blood.

Objective To explore the principles and methods of surgcal treatment for acute neoplastic colorectal obstruction.Methods The clinical data of 103 patients diagnosed as acute neoplastic colorectal obstruction were analyzed retrospectively.Results In this group,80 cases underwent emergency surgical treatment,(among) which,25 cases of carcinoma of right colon underwent primary resection and anastomosis;in 45 cases of carcinoma of left colon,primary resection and anastomosis was performed in 37 cases;and emergent(operations) were performed in 10 cases of rectal cancer.23 cases received non-emergency surgery.(Anastomotic) fistula occurred in 3 patients,pulmonary infection in 2 patients,pelvic cavity infection in 1(patient),1 patient died and the rest recovered well and were discharged.Conclusions The initial treatment for acute neoplastic colorectal obstruction should be non-surgcal treatment for 1~3d to make non-emergency surgical treatment more possible.If indications are properly selected,primary tumor resection and anastomosis is safe.For patients with severe contamination of abdominal cavity and bowel perforation, primary tumor(resection) should be performed if possible.