1.Study of the relationship of extracellular matrix and cell adhesion molecule with the damages of vas- cular basement membrance and endothelial cell in diabetic retinopathy
Fangdu LI ; Qiaomei CHU ; Shuling YAO
Chinese Journal of Diabetes 1994;0(02):-
0. 05, respectively). Conclusion The results suggest that the combination measurement of serum levels of IV C, LN and sVCAM-1 in DR patients might better evaluate the development process of diabetic mi-croangiopathy, and be of help for early diagnosis and therapy of diabetic microangiopathy. Serum levels of IVC, LN and sVCAM-1 in DR patients may be reliable clinical markers for assessing the severity and prognosis of diabetic retinopathy.
2.Neuroprotective effect of sodium oxybate against focal cerebral ischemia-reperfusion injury and the relation to GABA in rats
Mei LI ; Jiayong CUI ; Shuling GU ; Bing YAO ; Shimin DUAN
Chinese Pharmacological Bulletin 1987;0(03):-
AIMTo study the protective effect of sodium oxybate (SO) against focal cerebral ischemia-reperfusion injury in rats, and the relationship between the effects of SO and ?-aminobutyric acid (GABA). METHODSThe reversible middle cerebral artery occlusion (MCAO)model in rats was established to investigate the role of SO. The scores of neurological deficits was detected by Longa EZ method in MCAO rats. The extracellular levels of glutamate (Glu) and GABA in CSF were measured by high performance liquid chromatography-fluorometer (HPLC-FR) method, and the weight of cerebral infraction was detected. RESULTSThe scores of neurological deficits and the weight of cerebral infraction markedly decreased by SO while the ratio of GABA/Glu obviously increased administered SO in MCAO rats. CONCLUSIONSSO could prevent MCAO rats from ischemia-reperfusion injury, the protective effect is related to SO keeping dynamic balance of excition-inhibition, and persisting inhibition-depended effect.
3.Relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia-reoxygenation and GABA_A receptor in primary cultured rat cortical neurons
Shuling GU ; Mei LI ; Jiayong CUI ; Bin YAO ; Tijun DAI ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2002;0(05):-
AIM: To investigate the relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia reoxygenation and GABA A receptor in primary cultured rat cortical neurons. METHODS: The primary cultured rat cortical neurons were used to make the hypoxia reoxygenation damage model. The morphology of cell was observed. The lactate dehydrogenase (LDH) effluxed into the media as an indicator of neuronal injury was detected after 6 h of the reoxygenation injuries. The malonyldialdehyde (MDA) contents, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were determined at the same time. RESULTS: The hypoxia reoxygenation caused neuronal swelling and widespread neuronal degeneration, increased LDH efflux and MDA contents, and decreased SOD and GPX activities. Sodium oxybate assuaged neuron damage, decreased LDH efflux and MDA contents (P
4.Nephrectomy and removal of inferior vena cava tumor thrombus under profound hypothermia and arrested circulation
Yousheng YAO ; Jian HUANG ; Hai HUANG ; Yanqi YANG ; Shuling PENG ; Kewei XU ; Zhenghui GUO ; Tianxin LIN ; Chun JIANG ; Jinli HAN
Chinese Journal of Urology 2008;29(5):300-302
Objective To study the feasibility and safety of performing nephrectomy together with the removal of complicated inferior vena cava tumor thrombus under profound hypothermia and arrested circulation. Methods After made the median thoraco-abdominal incision, the exploration of the abdominal organs was done. The right kidney, inferior vena cava and renal pedicle were well exposed then. After the whole body heparinization, cannulas were put into ascending aorta, superior vena cava, aortic root and right superior pulmonary vein. The body temperature was reduced to 20℃ with cardiopulmonary bypass unit and the extracorporeal circulation was stopped then. Cut open the inferior vena cava at vena renalis dextra ingress and the F16 urinary catheter was inserted into atrum dextra through inferior vena cava and inflated. The tumor thrombus was pulled out and the right kidney was removed. The inferior vena cava incision was sutured to close and the extracorporeal circulation was resumed and patient was re-warmed.Results The operation time was 330 min and the extracorporeal circulation time was 90 min, while the profound hypothermia with circulatory arrest time was 20 min. The estimated blood loss during operation was 400 ml and 6 unit red cells and 600 ml blood plasm were transfused. The patient was awaked 2.5 h after the operation, food intake resumed 4 days after operation and the patient was discharged on day 10 post-operatively. After 6 months'follow-up, there were no local recurrence and metastasis occurred. Conclusion The technique of profound hypothermia and circulation arrest could improve the safety and efficacy in the treatment of renal cell carcinoma with suprahepatic (level Ⅲ) caval tumor thrombus.
5. A few thoughts on the postgraduate training for viral hepatitis
Lu ZHANG ; Yao LU ; Hongxiao HAO ; Shuling WU ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2017;31(4):372-374
Currently, there are two kinds of academic degree and professional degree in viral hepatitis postgraduate degrees. This kind of trainingmethod cannot combine clinical thinking and scientific research thinking. The combination of good research thinking and clinical foundation is beneficial to talent cultivation. In the future, the training of viral hepatitis graduate students requires students to have a clinical basis, and also requires students to have rigorous research thinking.
6. Improvement of the teaching methods of infectious diseases in the Internet era
Shuling WU ; Lu ZHANG ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2018;32(4):416-417
With the rapid development of network communication, the traditional teaching mode of infectious diseases can not meet the needs of new age. Network teaching has been paid more and more attention, showing the incomparable advantages of traditional teaching.
7. Factors associated with efficacy of nucleos(t)ide analogues with sequential interferon therapy in HBeAg negative chronic hepatitis B patients
Yuanjiao GAO ; Ge SHEN ; Yao LU ; Lu ZHANG ; Shuling WU ; Hongxiao HAO ; Min CHANG ; Leiping HU ; Wenhao HUA ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2018;32(5):533-537
Objective:
To study the factors associated with efficacy of nucleos(t)ide analogues with sequential interferon in HBeAg negative chronic hepatitis B (CHB) patients.
Methods:
HBeAg negative CHB patients with NA treatment received PEG-IFNα 2a 180 μg subcutaneously once weekly. NA was continually used with PEG-IFN 2a during the first 12 weeks. HBsAg level and HBV DNA load were observed in the sequential pre-treatment (baseline), 12th, 24th, 36th, 48th, 72nd and 96th weeks of sequential therapy in all patients.
Results:
Of the 26 HBeAg negative CHB patients, 6 (23.1%) achieved HBsAg loss/seroconversion. The comparison between HBsAg loss/ seroconversion group and the group not achieved HBsAg loss/ seroconversion showed that the baseline HBsAg level in HBsAg loss/seroconversion group was 2.210 log10IU/ml, was lower than (
8. Changes of APRI score in HBeAg-negative treatment-naive chronic hepatitis B patients receiving 3 year entecavir therapy
Ruyu LIU ; Lu ZHANG ; Yao LU ; Ge SHEN ; Shuling WU ; Mi CHANG ; Hongxiao HAO ; Yuanjiao GAO ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2019;33(3):309-313
Objective:
We aimed to evaluate changes towards liver fibrosis during entecavir(ETV)treatment by non-invasive fibrosis markers in chronic hepatitis B (CHB) patients who need antiviral therapy.
Methods:
Totally 303 HBeAg negative treatment-naive CHB patients were enrolled and liver biopsy was performed before starting antiviral therapy in this study. Totally 196 patients who need antiviral therapy were treated with ETV for at least 3 years. A clinical and virological evaluation was performed at baseline and again after 1, 2 and 3 years during ETV treatment. AST-to-platelet ratio index (APRI) was used to assess dynamic changes of liver fibrosis in HBeAg negative CHB patients after 1, 2, 3 years of ETV treatment.
Results:
All enrolled patients experienced liver biopsy at baseline. According to Metavir fibrosis stages, F1, F2, F3 and F4 patients were 107, 125, 54 and 17, respectively. The APRI score enabled the correct identification of patients with severe fibrosis (METAVIR F3-F4). The APRI values significantly decreased in F2 and F3 patients after 1 year ETV therapy (
9. Factors associated with efficacy of inucleoside analogues on sequential interferon therapy in HBeAg positive chronic hepatitis B patients
Yuanjiao GAO ; Lu ZHANG ; Yao LU ; Ge SHEN ; Shuling WU ; Hongxiao HAO ; Min CHANG ; Leiping HU ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2019;33(5):513-517
Objective:
To study the factors associated with efficacy of nucleos(t)ide analogues with sequential interferon in HBeAg positive chronic hepatitis B (CHB) patients.
Method:
HBeAg positive CHB patients treated with nucleoside analogue (NA) treatment received PEG-IFN α-2a 180 μg subcutaneously once weekly.NA was continually used with PEG-IFNα-2a during the first 12 weeks. HBsAg/HBeAg level and HBV DNA load were observed in the sequential pre-treatment (baseline) period, 12 th, 24 th, 36 th, 48 th and 72 nd weeks of sequential therapy in all patients.
Result:
Of the 56 HBeAg-positive CHB patients, 5 (23.1%) achieved HBsAg loss/seroconversion, the baseline HBsAg level in HBsAg loss/seroconversion group was lower than that of the patients in the group that did not achieve HBsAg loss/seroconversion (2.750 lg IU/ml vs. 3.699 lg IU/ml,
10. Changes in peripheral blood during the antiviral treatment with Peg-IFNα-2 a and ribavirin for patients with chronic hepatitis C and the relationship between the changes and effect
Zhenzhen LI ; Hongxiao HAO ; Lu ZHANG ; Yao LU ; Weihua CAO ; Xingyue WANG ; Chongping RAN ; Tianlin QI ; Shuling WU ; Ge SHEN ; Ru LIU ; Min CHANG ; Leiping HU ; Minghui LI ; Yao XIE
Chinese Journal of Experimental and Clinical Virology 2017;31(6):545-548
Objective:
To observe the changes of peripheral blood image of chronic hepatitis C (CHC) patients treated with pegylated interferon (Peg-IFN) and ribavirin, and explore the relationship between the changes and serum virological respose (SVR).
Methods:
Patients with CHC treated with Peg-IFN α-2 a and ribavirin in the Second Division of Liver Disease in Beijing Ditan Hospital were monitored for peripheral blood cells routine examination and Liver function, kidney function, thyroid function at baseline and week 2, 4, 8, 12, 24, 36, 48, and week 12, 24, 48 after the end of treatment for chronic hepatitis C, and HCV RNA.
Results:
The decrease of peripheral blood cell counts began to appear at week 2 of treatment. For CHC patients without cirrhosis, white blood cells, lymphocyte, hemoglobin and platelets at week 2, while minimum values were seen at week 36, and the neutrophils reached the minimum value to at week 24. Significant recovery of the peripheral blood changes was seen at the end of treatment (48 weeks), and reached pre-treatment levels at week 48 after the end of treatment. For CHC patients with cirrhosis, white blood cells, lymphocytes, hemoglobin and platelets significantly decreased at week 2, while neutrophils reached a minimum value at 2 weeks. And hemoglobin and platelets reached a minimum value at 24 weeks, and the white blood cells reached the minimum value at weeks 24-36 besides lymphocyte reached the minimum value at week 36. Significant recovery was seen at the end of treatment (48 weeks), and the blood cell counts reached pre-treatment levels at 48 weeks after the end of treatment. For patients with CHC, hemoglobin decreased by more than 27.47% at week 4, which means that the patient would have a predictive significance for SVR, as well as the of PLT reduction by more than 36.96% at week 8.
Conclusions
During the treatment with Peg-interferon and ribavirin, the variation of blood picture has some predicting effect, which predicts the result of antiviral treatment.