Aim In order to improver the diagnostic rate of Prion disease, solve the lacking sources of nature PrP antigen. ,synthesize the human PrP peptide, prepare the antibody to PrP peptide further. Method A synthetic peptide with the sequence identical to the 15 residues of human PrP, as described by Kretzschmar[1], was synthesized by the solid-phase method. The synthetic peptide was coupled to bovine serum albumin(BSA)by the method of EDCI〔2〕. The polypeptide combined with BSA was used as antigen to immunize the rabbit and detected by immunomethod. Result The PrP polypeptide combined with BSA obtained immunogenicity and anti-PrP synthetic peptide antiserum was successfully obtained. Conclusion The preparation and application of human PrP synthetic peptide can substitute for nature PrP antigen partly. It has laid a foundation for further preparation of monoclonal antibody to PrP and the study of Prion disease.

Objective To observe the dynamic changes of urotensionⅡ (U Ⅱ) content in plasma and bronchoalveolar lavage fluid (BALF) of rats with chronic hypoxic pulmonary hypertension and to investigate the effects of hypoxia on the synthesis and secretion of U Ⅱ and the correlation of U Ⅱ with the mean pulmonary artery pressure (mPAP) and the partial pressure of arterial oxygen (PaO 2). Methods Male Wistar rats were randomly divided into control group and hypoxic groups. Model of chronic hypoxic pulmonary hypertension was established by normal barometric and discontinuous hypoxia. mPAP and RVdp/dt max of each rat were measured using right cardiac catheterization. PaO 2 was detected by blood gas analysis. U Ⅱcontents in plasma and BALF were detected by radioimmunoassay. Results During hypoxia, mPAP, RVdp/dt max , U Ⅱ content in plasma, and BALF increased in rats (P

OBJECTIVE:To observe the clinical efficacy of compound glycyrrhizin combining desloratadine in the treat?ment of chronic urticaria.METHODS:103patients with chronic urticaria were randomly divided into treatment group which were treated with compound glycyrrhizin plus desloratadine and controlled group which were treated with single desloratadine.The courses of treatment for both groups were2weeks.RESULTS:The total effective rates of the treatment group and the controlled group were96.15%and82.35%,respectively.There were significant differences between the2groups(P

Circulating tumor cells (CTCs) detections have unique advantages in the real-time personalized medicine of patients with tumors.The rarity and heterogeneity of CTCs in peripheral blood pose great technical challenge for CTCs researches.Most of technologies for detecting CTCs have been developed to enrich CTCs by virtue of physical properties or specific biological features of the cells at first,then genotype or phenotype analysis are performed to count or characterize CTCs.Advances in molecular analysis of single cells enable CTCs detections to provide more accurate and comprehensive information.Biological processes during CTCs invasion and metastasis such as epithelial-mesenchymal transition and clustering should to be considered seriously to promote the clinical applications of CTCs detection.

As the global economic integration, the trend of internationalization of medical education, social demand for foreign talents is more and more big, the English teaching, especially the doctor-patient communication in English teaching has become a major focus on the teaching content.Learning is the study of doctor-patient communication be-tween medical workers and patients how to mutual understanding, trust and cooperation, common a discipline, to conquer the disease and emphasize ability oriented ESP teaching concept, closely integrated with professional, promotes the student as the center, apply English teaching as means and tools to study, the two organically unifies in together.Practice shows that guided by the ESP teaching concept, improve medical students English power of expression of doctor-patient commu-nication attempt made substantial results.

By analyzing the problems existing in the practice of doctor -patient communication , respectively discusses the speech act theory before the implementation of the implementation of doctor -patient communication oral English training , implementation , and after implementation of the positive role: instant help medical students to master a large number of medical English vocabulary;In view of the students come from different areas , instant help correct the pronunciation and intonation;Benefit based on speech act theory to guide the student to carry on the different roles played in English oral practice helps students to adopt direct and indirect speech act theory and the method of the oral English practice for communication;To improve medical students′professional recognition;To strengthen the medical students′sense of mission .

ObjectiveTo observe the effects of ultrafiltration extract from Angelica Sinensis Radix and Hedysari Radix on PC12 cell apoptosis induced by H2O2; To discuss its mechanism of action.Methods H2O2 was used in the incubation of PC12 cells to establish the oxidative damage nerve cell apoptosis model. The experiment was divided into normal control group, model group, and three different dosages (0.375, 0.75, 1.5 g/L) of ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix after modeling for interference. Rate of cell apoptosis was detected by flow cytometry; mitochondrial membrane potential was measured by laser scanning confocal microscopy; the protein expressions of cleaved Caspase-3, Bax and Bcl-2 were detected by Western blot.Results Compared with the model group, rates of cell apoptosis in the different dosage groups decreased significantly; membrane potential of mitochondria increased; the protein expressions of cleaved Caspase-3 and Bax decreased; the expression of Bcl-2 increased; the ratio of Bcl-2 and Bax increased (P<0.05).Conclusion The ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix can inhibit PC12 cell apoptosis induced by H2O2.

Objective To determine the optimal dose of dexmedetomidine for intravenous analgesia after open radical resection of intestinal neoplasms when mixed with flurbiprofen axetil and butorphanol.Methods A total of 120 patients of both sexes,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,aged 20-60 yr,weighing 45-80 kg,undergoing elective open radical resection of intestinal neoplasms,were divided into 4 groups (n =30 each) using a random number table:control group (group C)and different doses of dexmedetomidine groups (group DEX1,group DEX2,group DEX3).Group C received flurbiprofen axetil 2 mg/kg and butorphanol 0.05 mg/kg for intravenous analgesia.In DEX1,DEX2 and DEX3 groups,dexmedetomidine 0.3 μg/kg was intravenously infused starting from 30 min before the end of surgery,and the analgesia solution contained dexmedetomidine 1,2 and 3 μg/kg,respectively,which was mixed with flurbiprofen axetil 2 mg/kg and butorphanol 0.05 mg/kg in 100 ml of 0.9％ normal saline,and the mixture was infused at a rate of 2 ml/h.Butorphanol 0.5 mg was intravenously injected as a rescue analgesic,postoperative pain was assessed using the visual analog scale at coughing,and visual analog scale score was maintained ＜4.The requirement for rescue analgesics was recorded within 48 h after operation.The occurrence of postoperative adverse reactions such as nausea and vomiting,respiratory depression,somnolence,bradycardia,hypotension and over-sedation,patient's satisfaction with analgesia and length of postoperative hospital stay were recorded.Results Compared with group C,the rate of rescue analgesia after operation was significantly decreased,and the degree of satisfaction with analgesia was increased in DEX2 and DEX3 groups,and the incidence of postoperative somnolence was significantly increased in group DEX3 (P＜0.05).No other adverse effects were found in DEX1,DEX2 and DEX3 groups.Conclusion When mixed with flurbiprofen axetil and butorphanol,the optimal dose of dexmedetomidine for intravenous analgesia after open radical resection of intestinal neoplasms is 2 μg/kg.

Objective To investigate the expressions and significance of transforming growth factor-beta 1(TGF?1) and its typeⅡ receptor(TGF?RⅡ) in experimental rat ascending aortic aneurysm of rat model.Methods The rat ascending aortic aneurysm models were made by banding ascending aorta of young Wistar rats.The ascending aortas were taken 4 months after operation.Immunohistochemistry staining and Western blotting were used to investigate the expressions of TGF?1 and TGF?RⅡ.Result Immunohistochemistry staining results showed that TGF?1 expressed in all layers of the aortic aneurysm and the control.TGF?RⅡ was extensively located in the hyperplastic intima and tunica media smooth muscle cells in the aortic aneurysm,while there was only a little positive staining in the control group.Western blotting results indicated that the expression levels of TGF?1 and TGF?RⅡ in the aortic aneurysm were stronger than the control,P

I. Under scanning electron microscope, the vascular network of the cutaneous, subcutaneous, and deep fascia in planta can be divided into five layers according totheir vascular morphological characteristics. They are as following: the papillary layer, the subpapillary layer, the deep dermal layer, the subcutaneous layer, and the deep fascia layer. 2. The papillary layer is the most superficial one, it is easy to recognize that there are five types of capillary network: the single loop, the multiple loop, the tower-like type, the finger-like type, and irregular type. 3. The network in the subpapillary layer is polygon-like, parallel to the surface of skin. 4. In the deep dermal layer, there are many vascular balls in the vascular trunks. 5. The subcutaneous layer: The vascular networks are limited by fat lobules. 6. The vascular-trunks run across each other and anastomose each other in deep fascia layer.