1.Role of Tumor Necrosis Factor-Alpha Monoclonal Antibody in Cell Apoptosis after Combined Liver and Kidney Transplantation in Rats
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To investigate the potential role of tumor necrosis factor-alpha (TNF-?) in apoptosis after combined liver and kidney transplantation in rats. Methods Eighty rats which had combined liver and kidney transplantation were randomly paired, were divided into study group (n=20) and control group (n=20). 40 ml of 4 ℃ sodium chloride and anti-TNF-? monoclonal antibody (30 ml was infused from portal veins to donated livers and 10 ml from renal arteries to donated kidneys) were infused to the study group (0.1 mg/kg weight),and the same quantity of 4 ℃ sodium chloride was infused the control group. Venous blood was drew at different phases after the transplantations to detect the function of kidney and liver. The level of TNF-? and the cell apoptosis were detected in the transplanted tissues of liver and kidney by ELISA and terminal deoxynucleotidy transferase mediated dTUP-biotin nick-end labeling (TUNEL). Results The levels of AST, ACT, Cr and BUN in the study group were significantly lower than those of the control group at the same phases (P
2.Analysis of 53 Patients with Sjogren' s Syndrome and Idiopathic Pulmonary Fibrosis
Kuanting WANG ; Shuling HAN ; Min ZHANG
Journal of Medical Research 2006;0(11):-
Objective To explore the occurrence, clinical feature and the associatrd factors of Sjogren' s syndrome(SS) complicated with Idiopathic Pulmonary Fibrosis(IPF) to build up knowledge for early diagnosis. Methods The clinical characteristics of 160 cases damitted SS patients collected from January 2002 to August 2008 were retrospectively investigated including RF, chest - X, ESR, blood -gas analysis,pulmonary function tests,chest HRCT,ANA and so on. Results The patients with IPF accounted for 33.12% of total SS patients, including 12 cases of male. These patients presented strongly positive ANA,strongly positive RF etc,especially in the male. Conclusion The occurrence of SS - IP was related to the disease' s activity. FHRCT and pulmonary function test should taken early in order to get early diagnosis and treatment.
3.The expression ofp21~(WAF1)gene and p53 protein in tuberculosis with lung cancer.
Xiaomei FU ; Anjia HAN ; Shuling ZHU ; Al ET ;
Chinese Journal of Practical Internal Medicine 2006;0(S1):-
Objective To explore the role of cell cycle regulator in tuberculosis with lung cancer.Methods The ex- pression of p21~(WAF1)gene and p53 protein in 101 cases of tuberculosis with lung cancer have been detected by immunohis- toehemical method.Results total positive staining rate of p21~(WAF1)gene and p53 protein was 42.9% and 64.1%; The expression of both was correlated with histological classification and differentiation of carcinoma.Conclusion Detection of p21W AF1 gene and p53 protein expression may have significant value in the prognosis of tuberculosis with lung cancer
4.The cost-effectiveness analysis on standardized management of hypertension in community
Wenbin DENG ; Guanghu ZHAO ; Chao HAN ; Shuling LIAN ; Qiuting WANG
International Journal of Traditional Chinese Medicine 2012;34(7):594-597
Objective To discuss the effectiveness of standardized management of hypertension and its significance of economics.Methods Taking prospective,randomized and controlled trial design to screen hypertension in four communities of Changping Town.The patients in the four communities were randomly divided into an intervention group and a control group.The intervention group was taken the standardized management and the control group was taken conventional management.Results The treatment rate and control rate of the intervention group was higher than the control group.Necessary inputs of per capita administration costs for every 1% increase of treatment or control rate in the intervention group were less than the control groups by 1.08 and 1.36 Yuan respectively.The per capita administrative costs of intervention group were higher than control group,while the per capita comprehensive costs of intervention group were lower than control group.Compared with the control group,every 1 Yuan of per capita administrative costs increases,the comprehensive costs would be reduced 7.58 Yuan in the intervention group.Conclusion Standardized management of hypertension in community can significantly improve the prevention and treatment effects.Moreover,it has good cost-effective value and saving social resources.
5.Effect of Motor Imagery on Patients Accepted Radiotherapy after Operation for Cerebral Glioma
Jianying CHU ; Shuling HAN ; Haiyan WU ; Li CHEN
Chinese Journal of Rehabilitation Theory and Practice 2014;(6):571-575
Objective To observe the effect of motor imagery therapy on patients accepted radiotherapy after operation for cerebral glioma. Methods 34 patients were randomly divided into control group (n=17) and observation group (n=17). Both groups accepted routine physiotherapy during radiotherapy, 45~60 min/d, 5 times a week for 4 weeks, while the observation group underwent motor imagery additionally, 15 min/d for 4 weeks. They were evaluated with Fugl-Meyer Assessment (FMA), Manual Muscle Test (MMT) and modified Barthel Index (MBI) before and after treatment., and with Karnofsky Performance Scale (KPS) 1 year later. Results There was no significantly difference in the scores of FMA, MMT and MBI between 2 groups before treatment (P>0.05). There was no significantly difference in the score of FMA in both groups before and after treatment (P>0.05). The score of MMT improved in the observation group after treatment (P<0.01). The scores of MBI improved in both groups after treatment (P<0.05). And there was no significantly difference in the D-value score of FMA, MMT and MBI between 2 groups (P>0.05). After 1 year follow-up, KPS showed that the number of survival patients and their quality of life were better in the observation group than in the control group (P<0.05). Conclusion Motor imagery therapy can improve the muscle force, activities of daily living and long-term quality of life in patients during radiotherapy after neurosurgery for cerebral glioma.
6.Effect of polysaccharide sulfate on plasmatic P- selectin in patient with acute cerebral infarction
Yongjiu WANG ; Yihui SUN ; Zhenqing GONG ; Shiqiang BI ; Shuling WANG ; Zhenhua JI ; Zhongyan HAN
Chinese Journal of Postgraduates of Medicine 2006;0(28):-
0.05),but the level of P- selectin in treatment group were obviously decreased compared with control group after the seventh and fourteenth day(P
7.The relationship between serum copper/zinc ratio and classification as well as prognosis of Hodgkin's disease
Cunzhi HAN ; Shuling HOU ; Jingang GUO ; Xianwen ZHAN ; Jiexian JING ; Yua TIAN
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To clarify the relationship between serum copper/zinc ratio(CZR) and histopathological classification as well as prognosis of Hodgkin's disease (HD). METHODS: The serum copper level(SCL),serum zinc level(SZL)and CZR were determined by atomic absorption spectrophotometry(AAS)in 135 patients with HD and 100 healthy individuals.RESULTS: The results showed that SCL and CZR were higher and SZL was lower in patients with HD than in healthy individuals ( P
8.The relationship between serum copper/zinc ratio and classification as well as prognosis of Hodgkin's disease
Cunzhi HAN ; Jingang GUO ; Xianwen ZHAN ; Jiexian JING ; Yuan TIAN ; Shuling HOU
Chinese Journal of Pathophysiology 2001;17(5):458-460
AIM:To clarify the relationship between serum copper/zinc ratio(CZR) and histopathological classification as well as prognosis of Hodgkin's disease (HD). METHODS:The serum copper level(SCL),serum zinc level(SZL)and CZR were determined by atomic absorption spectrophotometry(AAS)in 135 patients with HD and 100 healthy individuals.RESULTS:The results showed that SCL and CZR were higher and SZL was lower in patients with HD than in healthy individuals (P<0.01). SCL and CZR were significantly higher in the patients with lymphocyte depleted type(LD)than mixed cellulal type(MC), SCL and CZR were higher in MC type than nodular sclerosis type(NS)and lymphocyte predominance type (LP)(P<0.01). It was also found that with the development of the disease, the SCL and CZR elevated but SZL dropped. And with the alleviation of the disease, the SCL and CZR rose while SZL dropped. While the illness is relieved and the SCL and CZR markedly dropped and SCZ rose. SCL and CZR were strongly correlated with histopathologic type and clinical stage of tumor.CONCLUSION:SCL, SZL and CZR are parameters useful for clarifying histopathological classification, clinical stage and prognosis of HD.
9.Effect of osimertinib combined bevacizumab on lung adenocarcinoma with EGFR T790M mutation and its mechanisms
Zhicheng XIONG ; Yang LIU ; Xin SUN ; Jietao MA ; Shuling ZHANG ; Li SUN ; Jing SUN ; Xiaonuo ZHANG ; Chengbo HAN
Chinese Journal of Clinical Oncology 2017;44(15):744-749
Objective:This study was performed using preclinical transplanted animal experiments to analyce the effects and mechanisms of third-generation EGFR-TKIs combined with anti-angiogenic therapy, thereby providing theoretical basis for further clinical trials. Methods:Researchers constructed the transplant BALB/C nude mice models with H1975 lung adenocarcinoma cell line (EGFR T790M) and divided the mice into four groups and treated them with osimertinib (2.5 or 5 mg/kg/day, gavage) alone or plus bevacizumab (5 mg/kg/twice weekly, i.p.) when the tumors reached approximately 0.4-0.6 cm3 in volume. The tumor growth curve of tumor volume was drawn according to the time in every group. After 2 weeks of treatment, the mice were killed and the tumors were processed for immunohistochemical staining and Western blot analysis. Immunostaining was performed to detect:HIF-1α, VEGF, and microvessel density (MVD) by using SP method on paraffin sections. Western blot analysis was used to analyze the protein expression levels of EGFR, AKT, and ERK signal transduction pathways. Results:After 2 weeks of treatment in high-and low-dose osimertinib alone, tumor volume in the high-dose group was significantly less than in low-dose osimertinib-alone group (P<0.05). VEGF, HIF-1αexpression, and MVD were significantly low in the high-dose osimertinib-alone group (P<0.05). Increasing doses of osimertinib induced dose-dependent weakening of the p-EGFR, p-AKT, and p-ERK expression levels (P<0.05). In the low-dose osimertinib-plus-bevacizumab group and low-dose osimertinib-alone group, no significant difference in tumor volume and the above factors was observed. In the low-dose osimertinib-plus-bevacizumab group and high-dose osimertinib-alone group, tumor volumes did not exhibit significant difference (P=0.178). Moreover, VEGF, HIF-1αexpression, and MVD exhibited no significant difference. No significant difference in the p-EGFR, p-AKT, and p-ERK expression levels was found between high-dose osimertinib-alone group and low-dose osimertinib-plus-bevacizumab group (P>0.05). In the high-dose osimertinib-plus-bevacizumab group, tumor growth was not significantly greater than that in the high-dose osimertinib-alone group (P=0.642). No significant difference was observed in the above factors.In the high-and low-dose osimertinib-plus-bevacizumab groups, tumor volume and the above factors did not exhibit significant differences (P>0.05). Conclusion:Osimertinib has obvious antitumor effects in EGFR-mutant lung adenocarcinoma with T790M mutation cell xenografts. Bevacizumab has a synergetic inhibitory effect with osimertinib against EGFR-mutant lung adenocarcinoma with T790M mutation cell xenografts. Bevacizumab enhanced the antitumor effects of osimertinib by reducing VEGF expression and the microvascular density of the tumor, thereby improving the tumor microenvironment. Bevacizumab can enhance the effect of osimertinib by suppressing EGFR, ERK, and AKT phosphorylation, thereby synergistically inhibiting EGFR activation and downstream signaling.
10.Correlated research between protein spectrometry and lymphoma markers for DLBCL patients
Qi ZHANG ; Qiaohua ZHANG ; Shuling HOU ; Jun WANG ; Jianxia HE ; Sutong GUO ; Yuping ZHENG ; Weie HAN ; Lieyang WANG ; Bin YANG
Journal of Leukemia & Lymphoma 2008;17(2):94-97
Objective To research correlation of serum protein spectrometry and lymphoma markers for diffuse large B cell lymphoma (DLBCL) patients. Whether there is relative between the protein and prognosis will be further researched. Methods Serum protein spectrometry of 62 DLBCL patients was detected by the SELDI-TOF-MS technique and Weak cation exchange 2 (WCX2) chip. Lactate dehydrogenase (LDH) was detected by biochemistry method. Beta-2-microglobulin (β2-MG) and cancer antigen125(CA125)were detected by enzyme-linked immunosorbent assay (ELISA). The level of LDH, β2-MG and CA125 for DLBCL patients between 11×103~12×103 protein expressed positively and negatively was analyzed. Meanwhile,correlation analysis and survival analysis were done. Results LDH, β2-MG and CA125 in 11×103~12×103protein expressed positive group were (523.30±435.96)U/L, (3.23±1.24)mg/L, (81.07±61.39)U/L respectively,and they were higher than that in negative group (P<0.05). 11×103~12×103 protein was positive correlated to LDH, β2-MG and CA125 (P<0.01). The survival time in 11×103~12×103 protein expressed in positive group,in which median survival time was 11 months, was shorter than that in negative group(P <0.01). The survival time in LDH normal group was longer than that in increased group(P <0.01). The survival time of β2-MG and CA125 had no significant difference between increased group and normal group. Conclusion LDH and 11×103~12×103 protein are expected to be prognosis indicators for DLBCL patients.