Objective To understand whether phenol oxidase (PO) existed in the body of Oncomelania hupensis and to observe histological distribution of the enzyme. Methods Enzyme histochemical method: adult female and male snails collected from the riverside of Yangtze River of Wuhu section were anatomized and consequently incubated in pH 6.8 phosphate buffer containing 25 mmol/L catechol at 37 ℃. Then under a stereo microscope, some of the snails were observed and recorded for the existing condition and histological distribution of PO in their bodies. Fluorescent histochemical method: after the process of incubation mentioned above, catechol were exsucted from the group of other snails. Afterward, some phosphate buffer solution (pH 6.8) containing 0.05% sodium penobarbital was added into the group. Then the existing condition and histological distribution of PO were also observed and recorded under a fluorescent microscope. Results The first method indicated that the color of the liver surface turned from French grey to light grey in 5 minutes, to grey in 15 minutes and black in 30 minutes. The second method showed that fluorescien appeared on the surface of the snail liver. Conclusion PO exists in the bodies of male and female snails and locates in their livers.

Objective:To study the expression of TCRV? subfamily which specially recognize the hepatoma cell antigen and the apoptosis of hepatoma cell induced by McAb costimulated PBLs.Methods:The change of the phenotype of PBLs was studied by flow cell cytometry and the level of the expression of TCRV? was studied by RT-PCR and Southern blot,the PTK by western blot.The hypermicroscopic ultrastructure was observed through transmission electron microscope.Results:The level of CD3 and CD8 of PBLs was significantly increased after acted with hepatoma cells,while there was no change in CD4.The expression of TCRV?7 of PBLs was dramaticly increased and peaked at 4 days.PTK increased correspondently,to 58% compared with 11% in control.Besides anti-CD3 McAb induced lymphocyte apoptosis,the mediated apoptosis of hepatoma cells was found in the other three groups.Conclusion:TCRV?7 was the tumor antigen specific T cell receptor,and it activate the PTK signal pathway.The McAb activated lymphocytes initiated apoptosis in hepatoma cells.

Objective To investigate the histological distribution of phenol oxidase (PO) in body of Oncomelania hupensis. Methods Oncomelania hupensis snails collected from the riverside of Wuhu section of the Yangtze River were anatomized and the unimpaired software organs were sliced in frozen condition to get the continued sections, then the sections were incubated in pH 6.8 phosphate buffer containing 25 mmol/L catechol at 37 ℃ and investigated systematically for histological distribution of PO in snail bodies. Results The light microscope showed that there were black grannles of PO (histochemical reaction) in the liver, ctenidium, head-foot, tentacle and mantle collar of snail body. Conclusion PO exists in Oncomelania hupensis and locates in the liver, mantle collar, head-foot, tentacle and ctenidium.

Objective To explore the relationship between the Yangtze River floodplain ecological environment(vegetation, soil,water and light intensity)and the distribution of Oncomelania hupensis snails,so as to provide the evidence for ecological snail control. Methods Three regions(the Lu-Gang Bridge,Dragon Nest Lake in the bund,and Dragon Nest lake beach)were selected to investigate the plant characteristics(species,height,coverage,frequency and strain of clusters),soil characteristics (temperature,humidity,light intensity)and pH value. All the results were analyzed statistically with SPSS 18 software. Results A total of 920 boxes were investigated. The vegetation coverage was 3.7%-63.5%,and the dominant population was Cyperusrotun-dus L. cluster on the marshland. The soil temperature was 19.0℃-24.0℃,pH 5.0-5.7,and humidity 53%-75%. There were statis-tical significants in average number of living snails and dead snails among 3 groups( P<0.05). As the light intensity was strong in summer and weak in winter,the snails were in the back surface in summer and front surface in winter respectively. The average number of living snails was the most near the water. The difference was statistical significant(P<0.01)among three ranges(0-1 m,1-3 m and 3-5 m). Conclusion The snail survival and distribution have close relations with micro ecological environment factors,such as vegetation,soil,water and light intensity.

Objective To synthesize a new reagent, 1-(6-bromo-2-benzothiazolyl)-3-(5-bromo-8-quinolyl)-triazene (BBTBQT) and apply to the determination of cobalt. Methods The reagent had been synthesized by diazotization and coupling reaction. After purification and characterization, BBTBQT was tested for its color reaction conditions with cobalt in the presence of cetylpyridinium bromide (CPB). Results In the presence of borax buffer solution at pH value of 9.0, the reagent reacted with cobalt to form a blue stable complex with a molar ratio of 2∶1; the complex had a maximum absorption at 640 nm. The apparent molar absorptivity of the complex is 1.55 ?105 L/(mol?cm). Beer’s law was obeyed in the range of 0-0.28 mg/L for cobalt. The method had been applied to determine cobalt in drainage sediment and vitamin B12 injection solution with a mean relative error of 1.9%-5.8% and a standard relative deviation of 1.7%-3.8%. Conclusion The present method is selective, sensitive, accurate, convenient and suitable for determination of trace cobalt in the samples.

Objective To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City so as to provide the evidence for its prevention and control. Methods From March to June and September to De?cember in 2014 dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated identified and counted. Results Totally 360 samples were selected and 169 samples 46.94% had mite infestation. Pyroglyphidae accounted for the most in the population with the breeding rate of 45.00%. Totally 18 504 mites were found and Dermatophagoides farina and Dermatophagoides pteronyssinus were the dominant species with the composition ratios of 30.21%5 590 mites and 17.83% 3 300 mites respectively. The third?ranked kindergartens were in the most serious situation con?cerning with 81.67% 98/120 of the breeding rate of Acaroid mites. The differences were statistically significant among the kin?dergartens with different ranks F=6.048 χ2=73.523 both P<0.05 . There were no significant differences about the Aca?roid mite population among different grades in kindergartens F=0.132 χ2 =2.377 both P>0.05 . Conclusion Acaroid mites in the kindergartens in Wuhu City were in serious condition and the population and species composition of Acaroid mite community is influenced by the human behavior and indoors environments.

Objective To analyse and solve the difficulties and countermeasures in continuously proving medical gas. Methods Aiming at the difficulties of continuously proving medical gas, oxygen produced are mostly canned by oxygen compressor, and are transported to every function module through connecting oxygen jars. Results Medical gas module was taken into full play to meet reasonable demand for continuously providing oxygen. Conclusion It is suitable for other field module hospital to use for reference in the action of medical succor of disaster.

Objective To study the seasonal changes of glucose levels per unit soft tissue of Oncomelania hupensis. Meth-ods O. hupensis snails were collected from the beach of the Qingyi River in Wuhu City,Anhui Province from August 2012 to July 2013. They were kept in minus 80℃refrigerator immediately. The male snails were distinguished from female,and their soft tissues were collected separately after crushing their shells. The hexokinase method was used to measure the glucose concen-trations,and the results were analyzed statistically. Results The contents of glucose decreased from March to July. The lowest glucose content in the female was 1.87μg/mg in March,and that in male was 3.70μg/mg in July. Both of them increased from August and reached peak levels in September(♀=57.38μg/mg,♂=44.39μg/mg),and then gradually decreased from Octo-ber to next January and increased in February. Conclusion The contents of glucose have seasonal changes regularly in O. hu-pensis.

Objective To find out a quick,simple and convenient method of determining the viability of Oncomelania hupen-sis. Methods O. hupensis snails were stained for 30 minutes by 0.05%water soluble dye neutral red,0.5%methylene blue,red ink,methylene blue-eosin-borax(MEB)and 0.4%trypan blue,respectively. The soft tissue samples of the snails were observed by a stereoscopic microscope after crushing their shells. Results The vital snails were stained and the dead were unstained in the neutral red. The vital and dead snails were unstained in methylene blue. However,the vital and dead snails were stained in red ink. The partial vital and dead snails were stained in MEB. The vital snails were stained and the partial dead were stained in trypan blue. Conclusion The use of 0.05%water soluble dye neutral red is simple,rapid and accurate in determination of the viability of O. hupensis.

Collagen is a kind of natural biomedical material and collagen based three-dimensional porous scaffolds have been widely used in skin tissue engineering. However, these scaffolds do not meet the requirements for artificial skin substitutes in terms of their poor mechanical properties, short supply, and rejection in the bodies. All of these factors limit their further application in skin tissue engineering. A variety of methods have been chosen to meliorate the situation, such as cross linking and blending other substance for improving mechanical properties. The highly biomimetic scaffolds either in structure or in function can be prepared through culturing cells and loading growth factors. To avoid the drawbacks of unsafety attributing to animals, investigators have fixed their eyes on the recombinant collagen. This paper reviews the the progress of research and application of collagen-based 3-dimensional porous scaffolds in skin tissue engineering.
Animals ; Biocompatible Materials ; Biomimetics ; Cell Culture Techniques ; Collagen ; chemistry ; Porosity ; Skin ; Skin, Artificial ; Tissue Engineering ; Tissue Scaffolds