Attenuated Salmonella can be used not only as live vaccines but also as vectors for exogenous genes. As vectors, attenuated bacteria can deliver genes directly to tumor tissues, leading to the expression of the cloned genes and induction of specific anti-tumor immunologic responses to inhibit tumor growth. Using attenuated Salmonella as a vector offers a new approach for vaccine development.

Objective To explore the clinical features of hepatitis G virus (HGV) infection and HGV replication sites in vivo. Methods A sensitive and specific reverse transcription-nested polymerase chain reaction (RT-nested PCR) was set up to detect the plus-stranded and minus-stranded HGV RNA in sera of 129 high risk population as well as in peripheral blood mononuclear cells (PBMCs) from 59 cases of them. The clinical features of HGV infection were analyzed in the meantime. Results Among 9 patients with single HGV infected, 3 cases were diagnosed as acute hepatitis, 3 cases as chronic hepatitis and the other 3 cases as healthy carriers. The detection rate of HGV RNA in PBMCs is much lower than that of HCV RNA (35.7 % vs. 77.3 % , P＜0.05). In all 59 PBMCs and 129 serum samples no minus-stranded HGVRNA was found. Conclusion HGV infection can cause acute or chronic hepatitis with mild clinical manifestation. HGV can invade PBMCs but can not replicate in PBMCs, and the ability of HGV to invade PBMCs is lower than that of HCV.

Objective: To analysis the effects of laser and lucentis treatment of diabetic retinopathy. Methods:Fifty-three patients with DR were treated with laser and lucentis in our hospital from October 2012 to October 2013 as observer group. Forty-nine patients with DR were treated with laser in our hospital from September 2011 to September 2012 as control group. Results:The results indicated that visual acuity improved rate was 67.9%and decreased vision rate was 13.2% in observer group. Vision conditions in the observation group was better than that in the control group with significant difference (x2=4.60, x2=3.87; P<0.05). Simultaneously, improve time of hemorrhage, exudates, edema and macular thickness in the observation group was better than that in the control group with significant difference (t=4.17, t=3.92, t=4.06; P<0.05). Conclusion: The laser and lucentis treatment of diabetic retinopathy can significantly improving symptoms of retinal and promoting visual recovery.

Diabetic retinopathy (DR) is one of the microvascular complications of diabetes mellitus (DM).Like other macrovascular complications of DM,the development and progression of DR is influenced by a variety of systemic and local factors.It is essential to understand the importance of multidisciplinary collaboration.Systemic risk fators such as hyperglycemia,hypertension,dyslipidemia and diabetic nephropathy should be treated before effective DR management can be implemented.Through multidisciplinary collaboration,we can prevent the development of DR,slow the progression of DR,and improve the safety of perioperative care.Thereby enhancing the level of prevention and control of DM complications,including DR.

Objective To investigate the mechanism,clinical features of severe skin infection developed in the rehabilitation of the elderly diabetic patients and the commonly seen bacterium.Methods The causes of skin injury,the places of infection and bacterium were analyzed retrospectively.Meanwhile,chemical indicators on admission were analyzed.Results Skin injury was caused by the improper family care and the lake of self protection knowledge.The common places for infection were feet and then back and hip skin.Almost all the infections were caused by opportunists,among which staphylococcus was the most common one.The major separated risk factors were hypoalbuminemia,hyperglycemia,and high level fibrinogen and anemia.Conclusion The elderly diabetic patients are subject to skin infection.Diabetic education and careful nursing are the key to the prevention of severe skin infection.

Objective:Evaluating quality of life of comm unity people in Hangzhou city. Methods:492 community peopl e were sampled from community and tested by Generic Quality of L if e Inventory-74.Results:(1) Generic quality of life of comm unity people was well, and their body and mind were in good health; (2) Effects of mental health、physic health and social function on quality life were over ef fect of material life conditions;(3) Qualities of life of elders、workers、non-p rofession people were worse than qualities of young、profession people and cadre (P<0.01).Conclusion:Quality of life of community peop le of Hangzhou was in good condition, but attentions should be paid to qualities of life of elders and workers.

Objective To study the interaction between HCV core protein and HCBP6 in mammlian cells using CheckMateTM Mammalian Two-Hybrid System.Methods cDNA fragments encoding HCV core protein and HCBP6 were amplified by PCR and subsequently cloned into pGEM-T vector.After verified by sequencing,the target fragments were subcloned into mammalian two-hybrid plasmids,pBIND and PACT,respectively.The recombinant plasmids,pBIND-core and pACT-HCBP6 were co-transfected into HepG2 cells with reporter plasmid pG5luc.pBIND+pACT were induced as background controls,pBIND-Myod+pACT-Id as positive controls,and pBIND-core+pACT,pBIND+pACT-HCBP6 as blank controls.The expression of G5luc,which indicated the interaction between HCV core protein and HCBP6 in mammlian HepG2 cells,was assayed through Dual-Luciferase Report Assay System and Turner Biosystems Veritas Microplate Lunimometer.Results The recombinant vectors pBIND-core and pACT-HCBP6 were successfully constructed.When co-transfected into HepG2 cells with reporter plasmid pG5luc,there were significant differences in the luciferase activity in the pBIND-core and pACT-HCBP6 groups compared with every control group.Conclusions HCBP6 can interact with HCV core protein in HepG2 cells,which provides clues for further study on the function of HCBP6 and core proteins,and on the mechanism of HCV core in cell apoptosis and cancer transformation.

Objectives To study the effect and the mechanism of peripheral blood nuclear cells (PBMC) invaded by hepatitis B virus (HBV) on the artificial immunization in newborns Methods Fifty two newborns, whose mothers were hepatitis B surface antigen (HBsAg) positive, were immunized with hepatitis B immunoglobulin and hepatitis B vaccine (HBVac), and then followed for 7 months The newborns′ serum and PBMC HBV DNA was detected by nested PCR, hepatitis B surface antibody (HBsAb) was tested with solid phase radioimmunoassay PBMC from newborn were incubated with PHA and HBsAg The supernatant interleukin 2 (IL 2) level was mesured by enzyme linked immununosorbent assay (ELISA) Results The rate of vaccination failure was higher in the infants with PBMC HBV DNA positive than those with negative ( P

Objective To study the therapeutic effect of ankylosing spondylitis treated with sacroiliac joint injection under C-arm X-ray guidance. Methods Four hundred and eighty-three patients of ankylosing spondylitis were divided randomly into two groups according to the treatment method. Four hundred and twenty-one cases were in group A:sacroiliac joint injection under C-arm X-ray, the injection medicine were diprospan or triamcinolone acetonide and methotrexate, and with oral sulfasalazine (SASP) as well. Sixty-two cases were in group B: oral medicion SASP and meloxicam alone without injection. Self-evaluation, finger-to-floor distance, changes in Schober's sign, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) before and after treatment of 3 d, 1 week,2 weeks, 3 weeks, 4 weeks and 3 months were observed. Results Two indicators including self-evaluation and finger-to-floor distance at initial 3 d were significantly lower than those before treatment in group A: (6.18 ±0.55) scores vs. (8.89 ±0.53) scores, (30.10 ±3.94) cm vs. (50.20 ±3.93) cm, P<0.05. Schober's sign and occiput-to-wall distance at 1 week after treatment were significantly lower than those before treatment in group A:(3.44± 0.32) cm vs.(3.19±0.47) cm, (9.49±0.68) cm vs. (10.72±2.36) cm, P<0.05. Two indicators including self-evaluation and finger-to-floor distance at 1 week after treatment were significantly lower than those before treatment in group B:(7.07 ± 0.72) scores vs. (9.08 ±0.46) scores, (48.14 ±5.62) cm vs. (50.84 ±4.33) cm, P<0.05. Schober's sign at 4 weeks after treatment was significantly lower than that than before treatment in group B:(3.49± 0.56) cm vs. (3.22±0.58) cm, P<0.05. Occiput-to-wall distance at 2 weeks after treatment was significantly lower than that before treatment in group B:(9.08±1.54) cm vs. (10.11±1.58) cm, P<0.05. Conclusion The method of C-arm X-ray guided sacroiliac joint injection for treatment of ankylosing spondylitis is effective and feasible.

Aim: To study the effect of Eudragit S-100, a pH-responsive polymer, on protein refolding level, using recombinant human keratinocyte growth factor-2 (rhKGF-2) as a model protein. Methods: The refolding of rh-KGF-2 was performed by directly diluting denatured rhKGF-2 into a refolding buffer containing different concentrations of Eudragit. The ability of Eudragit S-100 to enhance protein refolding level was investigated using MTT assay, reverse phase HPLC, fluorescence emission spectroscopy and circular dichroism spectroscopy. Results: The addition of Eudragit S-100 in the refolding buffer significantly increased the rhKGF-2 refolding yield to 71%, when dilution refolding was conducted at 0. 5 mg/mL rhKGF-2. The outcome from the refolding study showed possibility of a special interaction between rhKGF-2 and Eudragit, suggesting that the refolding-enhancing ability of Eudragit S-100 was due to this interaction between Eudragit S-100 and rhKGF-2. Mean while, the result showed that the concentration of urea was also an important factor for the optimization of the refolding in the presence of Eudragit. Conclusion: Eudragit S-100 can significantly increase the refolding level of rhKGF-2.