Objective To investigate the relationship between early brain menifestation such as white matter damage and ventriculomegaly detected by magnetic resonance imaging(MRI) and adverse neurodevelopmental outcome in preterm infants.Methods From March 15,2007 to April 12,2011,122 preterm infants accepted MRI examination 8-14 days after birth in Chinese People's Liberation Army 202 Hospital.Totally,115 preterm infants were followed up at 12 months old and mental development index(MDI) and psychomotor development index (PDI) score were measured.MDI＜70 was as mental dysfunction,-84 as borderline dysfunction and-114 as normal; PDI＜70 was as psychomotor dysfunction,-84 as borderline dysfunction and-114 as normal.The relationship between clinical characteristics of preterm infants,MRI abnormalities and neurodevelopmental outcome were analyzed by analysis of variance,LSD and Kruskal-Wallis H test.Results The 122 premature infants included 69 males and 53 females with the median gestational age of 32 weeks(28-36 weeks)and the median birth weight of 2050 g (1270-3110 g).In 24 premature infants with mentaldysfunction,the average gestational age [(28.7 ± 1.7) weeks],birth weight[(1520.1-44.8) g] and 1 min Apgar score (5.5 ± 0.8) were all lower than those in normal infants [n =59,(33.5 ± 2.2)weeks,(2240.4 ± 47.1) g and 7.1 ± 0.8],while the average mechanical ventilation time was longer [(20.4±5.8) dvs (5.6±2.7) d](t=2.37,2.49,2.13 and 2.44,P＜0.05).In 20 premature infants with psychomotor dysfunction,the average gestational age [(27.9 ±± 1.4) weeks],birth weight [(1515.6±43.7) g],1 min Apgar score (5.6t0.5) were lower than those in normal infants [n=62,(33.2±2.4) weeks,(2264.3±42.5) g and 7.2±0.6],while the mechanical ventilation time was longer [(18.2±4.7) dvs (5.3±2.2) d](t=2.28,2.52,2.09 and 2.38,P＜0.05).Among thirteen preterm infants with severe white matter damage,eleven and nine developed mental or psychomotor dysfunction respectively.Among eleven preterm infants with moderate and severe ventriculomegaly,seven and six developed mental or psychomotor dysfunction respectively.The more severe the white matter damage and ventriculomegaly,the higher the incidence of mental (H=16.23 and 14.33,P＜0.05) and psychomotor dysfuction (H =18.63 and 12.69,P ＜ 0.05).Conclusions White matter damage is common in preterm infants.Prognosis of preterm infants with mental and psychomotor dysfunction is related with the degree of white matter damage and ventriculomegaly.

To compare the varieties and contents of the main nerval information molecules in perfusate from hypothalamic medial preoptic area (MPOA) of the rats in different sexual cycles and the ovariectomized rats treated by electro-acupuncture, so as to observe the similarities and differences of hypothalamic neuroendocrine signal transduction pathway under the physiological and pathological status, and to explore the mechanisms of neuroendocrine signal transduction of electro-acupuncture therapeutic effect in perimenopausal syndrome.

Objective To study the association of peptidylarginine deiminase 4 (PADI4) (padi4_94 and padi4_104) genetic single nucleotide polymorphism (SNP) and rheumatoid arthritis (RA) susceptibility in Han population of Hebei province. Methods This hospital-based case-control study included 115 RA patients and 106 healthy controls. All the individuals were recruited from Han Hebei residents and were randomly selected. The genotype and allele frequencies of PADI4 gene polymorphisms (padi4_94 and padi4_104) were analyzed by PCR-DNA sequencing method. Results The distribution of padi4_94 and padi4_104 genotypes between the two groups was not significantly deviated from that expected by Hardy-Weinberg equilibrium (P>0.05). The combined effect of padi4_94 and padi4_104 SNPs was analyzed and three haplotypos (AA, AG and GG) were found but without GA haplotype. The genotype and allele distribution of padi4_94 and padi4_104 in the patients with RA was not significantly different from that in healthy controls,and the analysis of haplotypes revealed that no haplotypes were risk factors for RA. Conclusion In Han population of Hebei province, the SNPs of PADI4 (padi4_94 or/and padi4_104) is no associated with RA susceptibility. Therefore, it should not be regarded as a genetic risk factor for RA.

Objective To observe the clinical efficacy of acupuncture plus auricular point sticking in treating polycystic ovary syndrome (PCOS). Method A total of 80 PCOS patients were randomly divided into a treatment group of 40 cases and a control group of 40 cases. The treatment group was intervened by acupuncture plus auricular point sticking, while the control group was intervened by acupuncture only. The clinical efficacy was observed in the two groups after 3 treatment courses. Result The menstrual condition and basal body temperature (BBT) were compared in the two groups before and after the treatment. The menstrual condition and BBT in the treatment group were significantly improved (P<0.01); the menstrual condition was improved (P<0.05) and the BBT condition was significantly improved in the control group (P<0.01). The total effective rate was 82.5% in the treatment group, significantly different from that in the control group (P<0.05). Conclusion Acupuncture plus auricular point sticking can produce a satisfactory efficacy in treating PCOS.

OBJECTIVETo calculate the consequence of acute ammonia poisoning accident which was caused by the leak of liquefied ammonia storage tank, so as to provide theoretical basis for working out the counterplan of accidents.

METHODThe amount of liquefied ammonia to be reserved, the pressure and temperature inside the tank were inputted; based on the physical chemistry principle, the evaporating amount was calculated; and using the proper diffusion model, the size of ammonia cloud, percentages of death and depth range of the poisonous gas etc was calculated.

RESULTSAssuming the leaky tank had liquefied ammonia 50 t, the pressure and the temperature were 2.5 MPa and 30 degrees C respectively, the evaporating amount and the size of ammonia cloud were calculated. The radiuses, of the ammonia released cloud of severe, moderate and mild hazard were 108 m, 216 m, 370 m respectively; if the exposured time was 30 min, the percentage of death would be 50%; if the exposured time was 5 min, the percentage would be 10%; if the exposured time was 2 min, the percentage would be 2.5%. When the stable degree of circumstance was C, the wind speed was 2.3 m/s and the outside concentration of the cloud was 4000 mg/m3, dimension of cloud in X axis was 782 m and in Y axis was 88 m.

CONCLUSIONThe leaky consequence of liquefied ammonia was very serious. It is necessary to take preventive measures during production, storage, transportation and using of liquefied ammonia.

Objective To investigate the relationship between the PTPN22 gene polymorphism and rheumatoid arthritis(RA).Methods Real time fluorescent quantitation PCR was used to detect the 1123G＞C polymorphism of the PTPN22 gene from 200 RA patients,100 others rheumatic diseases and 200 the normal controls.The results were analyzed by SPSS 11.0 software.Results The CC genotype frequencies of RA patients.others rheumatic diseases and the normal controls were 0.120,0.020 and 0.015 respectively.There was a significant difiefence between BA patients and others rheumatic diseases (X=18.708.P＜0.01).1'here Wag a significant difference between RA patients and the normal controls(X2=24.337,P＜0.01).There was not statistically significant between others rheumatic diseases and the normal controls(X2=1.066,P＞0.05).The C allele frequency of RA patients,others rheumatic diseases and the normal controls were 0.360.0.190 and 0.215 respectively.The results were significant difference.Conclusion The PTPN22 gene could be one of predisposing genes and the therapeutic target genes with RA patients.

Objective To detect the value of autoantibedy profile in primary biliary cirrhosis (PBC). Methods 96 serum samples of patients with primiary biliary cirrhosis, 100 serum samples of other antoimmune disease and 49 serum samples of healthy were tested for anti- M2, anti-3E(BPO), anti-Sp100,anti-PML,anti-gp210,anti-LKM-1 ,anti-LC-1 ,anti-SLA/LP by EUROLine. Results The positive of the anti-M2,anti-BPO, anti-Sp100, anti-PML and anti-gp210 for PBC was 76. 0%, 84.4%, 32. 3%, 28. 1% and 35.4% ,respectively. The positive of the anti-M2, anti-BPO, anti-Sp100, anti-PML and anti-gp210 for other autoimmune disease was 13.0% ,9. 0% ,3.0% ,2.0% and 1. 0%, respectively. The sensitivity of the anti-M2 for PBC was 76. 0% ,with specificity of 87. 0%. The sensitivity of the anti-BPO for PBC was 84. 4%, with specificity of 91.0% ;the sensitivity of the anti-Sp100 for PBC was 32. 3%, with specificity of 97.0%. The sensitivity of the anti-PML for PBC was 28. 1% ,with specificity of 98.0%. The sensitivity of the anti-gp210 for PBC was 35.4%, with specificity of 99. 0% . Anti-LKM-1, anti-LC-1, anti-SLA/LP positive patients with PBC were not detected;the incidence rate of liver function failure in anti-gp210 positive serum higher than anti-gp210 negative serum (χ2 = 11.17, P < 0. 01). Conclusions Multiple autoantibedies can be detected in the sera of PBC patients. The detection of autoantibody profile is useful for the diagnosis and differential diagnosis of PBC, and may he helpful for therapy and prognosis of PBC.

ObjectiveTo investigate the effect of delayed cord clamping on outcomes of neonatal resuscitation.MethodsTotally, 7 429 full term infants born in the Maternal and Child Health Care Hospital of Jilin from January 2013 to January 2015 were randomly divided into two groups, the observation group (n=3 727) and the control (n=3 702). The cords were clamped 2 min delayed in the former group, while those in the latter group were clamped within 10 s after birth. The rate of asphyxia and successful resuscitation, Apgar score at 1 and 5 min after birth, and pH value, base excess (BE), blood glucose, cardiac troponin (cTn) I at 1 h after birth were compared witht-test orChi-square test.ResultsThe incidence of neonatal asphyxia in the observation group was lower than in the control [0.75%(28/3 727) vs 1.21%(45/3 702),χ2=4.115,P<0.05], but no significant difference was found in the rate of mild or severe asphyxia between the two groups [0.62% (23/3 727) vs 0.89% (33/3702), 0.13% (5/3 727) vs 0.32% (12/3 702), allP>0.05]. In observation group, those newborns who suffered from severe asphyxia had higher Apgar score at 1 and 5 min than control (1 min: 2.80±0.45 vs 2.08±0.67,t=2.181; 5 min: 8.00±1.00 vs 6.25±1.66,t=2.176; bothP<0.05). Higher blood pH value and lower glucose level were shown in the observation group than in the control group [7.16±0.41 vs 7.13±0.72, (5.91±1.19) vs (6.60±1.56) mmol/L,t=2.068 and 2.046, bothP<0.05]. However, no difference was found in BE and cTnI levels between the two groups (bothP>0.05). For those babies with severe asphyxia, the blood pH, BE, blood glucose and cTnI levels in the observation group were significantly different from the control group [7.08±0.29 vs 7.02±0.56, (-16.82±0.60) vs (-17.43±0.35) mmol/L, (7.93±0.78) vs (8.02±0.53) mmol/L and (0.203±0.041) vs (0.249±0.035) ng/ml,t=2.270, 2.387,-2.371 and-2.341, allP<0.05].Conclusion Two minutes delayed in cord clamping could help to reduce the incidence of asphyxia and improve the effect of resuscitation in severe asphyxia.

Objective To investigate the effects of acupoint catgut-embedding therapy(ACT) on hypothalamic of hypothalamic norepinephrine(NE),dopamine(DA),5-hydroxytryptamine(5-HT),5-hydroxyindole acetic acid(5-HIAA) in menopause rats,and to explore the preventive and therapeutic mechanism of ACT for climacteric syndrome.Methods Sixty female SD rats were equally randomized into treatment group,model group and sham-operation group.Except the rats in the sham-operation group,the rats in other groups received removal of bilateral ovaries to establish ovariectomized menopause rat models.The treatment group received catgut-embedding on acupoints of Shenshu,Sanyinjiao and Zigong,once per week,and the treatment lasted 4 weeks.After treatment,the hypothalamic NE,DA,5-HT,5-HIAA levels were measured by radioimmunoassay.Results NE and DA levels were significantly decreased(P

Recent years found a series of severe incidents of injuring or killing medical workers in several places in China,deteriorating patient-physician relations,and disturbing medical orders.Beijing Administration of Hospitals,since its founding,has attached great importance to the management of medical disputes,as evidenced in the full-process management covering pre-during-post disputes.The administration guided the hospitals in their dispute resolution,and included resolution rate of disputes as an index of municipal hospital performance evaluation.By means of the guidance of such evaluation, medical disputes and major medical accidents have been resolved satisfactorily,and fine management of service quality of such hospitals in terms of medical service,pharmacy,nursing and medical technology has been intensified all the time.