Objective To investigate the personality characteristics of the drivers in the Armed Police Force ( CAPF), as well as construct a personality traits model for drivers and evaluation criteria. Methods Adopting Sixteen Personality Factor Questionnaire(16PF) to collect data from 1187 CAPF drivers in Beijing,Sichuan,Inner Mongolia and Yunnan. Results The paper concludes that career personality traits of CAPF drivers are made up of 4 factors: stability and self-control, self-motivation and cooperation, fantasy and openness, intelligence and candor, which explain 50.13% of the total variance. Conclusion The four factors basically construct the personality trait model of CAPF drivers. They could also be of great significance in recruiting, training and evaluating drivers.

OBJECTIVE:To establish a HPLC method for the simultaneous determination of blood drug concentration of acetaminophen and phenacetin.METHODS:Diamonil C 18 was used as the chromatographic column,the mobile phase was methanol-water(45∶55),theophyllinum was used as internal standard,the detection wavelength was254nm and the column temperature was set at the room temperature.RESULTS:The minimum determination concentration of acetaminophen and phenacetin was5?g/ml,the calibration curve was linear in a range from5～25?g/ml;The recovery rate of both acetaminophen and phenacetin in this method was more than95%;both the intraday RSD and the interday RSD were less than4.0%.CONCLUSION:The method is rapid and accurate,which can satisfy the needs of the clinic blood drug concentration deter-mination of paracetamol and phenacetin.

Objective To establish an in vitro skin sensitization test,human cell line activation test (h-CLAT),based on THP-1 cell line (a human acute monocytic leukemia cell line),and to assess the sensitizing potency of plant raw materials of chemical and cosmetic products by this in vitro skin sensitization test.Method THP-1 cells were cultured in vitro and exposed to 11 reference skin sensitization chemicals and 9 samples,by monitoring the cell viability,cell surface marker CD54 /CD86 and relative fluorescence intensity of cells surface after the cells was exposures to the substances,and to discover whether there is a positive reaction.At the same time,Buehler test was used to validate the results of samples tested by h-CLAT.Results 11 reference chemicals were distinguished correctly by h-CLAT.Among the 9 samples tested,7 samples were recognized as negative sensitizer and 2 plant extracted substances were identified as suspicious skin sensitizer.The qualitative classification of the 9 samples by h-CLAT test was consistent with the results obtained by animal test.Conclusions The h-CLAT-in vitro test can be used to replace some animal tests for the prediction of soluble skin sensitizing substances.

Objective To develop a scientific system of core information of health and evaluation index for the elderly Chinese people,which is suitable for the situation of our country.Methods The framework was established by extensive literature review and topic discussions.The index system was constructed with the Delphi method.Results 58 experts in different fields of health were selected in the two rounds of Delphi consultation research.The effective response rates in the two rounds were 96.88％ and 100％ respectively.The average value of the authoritative coefficient was between 0.80 and 0.96.And coefficients of variation were between 6.20 and 12.13.Based on the Delphi consultation research,the core information of health and evaluation index system for the elderly Chinese people were composed of 8 primary indicators closely associated with the positive awareness of aging and senescence and 31 other indicators.Conclusions The architecture of the core information of health and evaluation index system for the elderly Chinese people is stable,and the consultant experts tend to reach a consensus.The evaluation index system contains the most influential factors for the elderly Chinese people,and has the affirmative scientificity,applicability and feasibility.

Objective To explore the use of integrated two methods in vitro in prediction of eye irritation caused by cosmetics.Method Chorioallantoic membrane vascular assay ( CAMVA), bovine corneal opacity and permeability (BCOP) and Draize rabbit eye irritation test were used to determine the predictive potential of eye irritation of 60 kinds of cosmetics.Results CAMVA method was able to distinguish 41 non-irritant samples and 18 irritant samples.BCOP method was able to predict 35 non-irritant samples , 21 mild-moderate irritant samples and 4 severe irritant samples . Combination of CAMVA and BCOP methods could obviously improve the identification ability of irritation , and the classification consistency with Draize rabbit eye irritation testing reached 98.3%.Conclusions The integrated test strategy combined BCOP with CAMVA can be used to appropriately predict ocular irritation of cosmetics , with a prediction range covering non-irritant to severe irritant samples .

Objective To investigate the prevention and treatment effects of Compound Kushen Injection on acute radiation esophagitis. Methods Eighty-two eligible patients with esophageal cancer were randomly divided into the treatment group (41 cases) and the control group (41 cases). All the patients received radiotherapy. Throughout the course of radiotherapy, patients in the treatment group received Compound Kushen Injection, and patients in the control group received Kangfuxin Liquid. Occurrence time and level of radiation esophagitis, and dosage of painkillers were observed. Results Different degrees of acute esophageal toxicity were observed in the two groups. The occurrence rate of high level (degree III and degree IV) acute radiation esophagitis was 7.3%(3/41) in the treatment group, and 31.7%(13/41) in the control group. There was significant difference between the two groups (P<0.05). The dosage of the analgesic drug (Fentanyl Transdermal System) in the treatment group was far less than the controlled group (P<0.001). Conclusion Compound Kushen Injection could decrease the incidence rate of acute radiation esophagitis, and reduce the high-level esophagitis and the dosage of the analgesic drug, which can help the completion of radiation.

Objective:To evaluate the feasibility of two materials used as scaffolds in periodontal tissue engineering. Methods: Dog periodontal ligament cells cultured in vitro were collected and seeded on three-dimensional framework of cancellous bone matrix (CBM) or nano-HAp/collagen (nHAC) . The cell growth in the scaffolds was observed by cell counting and scanning electronic microscope. Results: Porous structure of the two materials and adhesion and eugonic growth of cells in the scaffolds were observed by scanning electronic microscope.The cell number was doubled in both scaffolds in 72 h culture. Conclusion: It may be feasible to use CBM or nHAC as the scaffolds for periodontal tissue engineering.

Objective To establish a detection method integrating DPRA ( direct peptide reactivity assay) with h?CLAT ( human cell line activation test) to screen the skin sensitization potency of chemicals and plant extracts. Methods 12 chemicals and 7 plant extracts were chosen as the test substances. Firstly, the test substances were incubated together with two different peptides ( cysteine and lysine) respectively for reaction for 24 h. The peptide consumptions were analyzed by HPLC. Simultaneously, THP?1 cells were cultured in vitro and then exposed to different concentrations of test sub?stances for 24 h to examine the cell viability, cell surface markers CD54 and CD86 were assessed by flow cytometry. The predicting results were compared further between DPRA and h?CLAT. Results 12 chemicals were distinguished correctly by DPRA classified as 2 non?sensitizers and 10 sensitizers. The results of DPRA were in accordance with h?CLAT. Predic?ting the sensitization potency of plant extracts by DPRA showed that 6 plant extracts were determined as suspected sensiti?zers except for green tea extract. But using the method of h?CLAT, 4 plant extracts were examined as suspected sensitizers except for green tea extract, herba portulacae extract and ginseng fruit extract. The coherence of DPRA and h?CLAT was 0?57. Conclusion This detection method integrating DPRA with h?CLAT can predict single compound accurately. As for complex compound, it can achieve preliminary prediction and need other integrating methods to make a further identifica?tion.

The energy expenditure in the late stage of pregnancy was studied. The workers of three kinds including spinner, sewers and kindergartners were selected, and ten healthy pregnant women were matched to non-pregnant as subjects. Energy consumption was measured by indirect method and calculated from dietary survey. The results showed that the energy expenditure for spinner, sewer and kindergartner in pregnancy was 9.5474, 10.1253 and 8.7287 MJ/day, but for non-pregnant woman was 9.4453 10.1281 and 8.1567 MJ/day respectively. Calorie intake was 10.8910, 11.2424 and 9.9830 MJ/day for pregnant women, but 9.2215, 10.4474 and 8.6734 MJ/ day for the non-pregnant women respectively. The difference of energy consumptions between the pregnant and non-pregnant women was not significant, but the calorie intake for the pregnant women was signigicantly higher (0.8368 MJ/day) than the non-pregnant. According to the energy consumption, we recommend the calorie intakes in the late stage of pregnancy may increase 0.8368 MJ/day.

Objective:To clarify whether the signal peptide of human nerve growth factor can mediate secretory expression of beta-endorphin and whether there is difference between the efficiency of signal peptides from human and mouse nerve growth factor.Methods: Two kinds of eukaryotic vectors containing human or mouse signal sequence-mediated secretory expression of beta-endorphin were constructed.The culture supernatant and cells were collected 48 h after NIH3T3 cells were transfected by the two kinds of vectors,and the cover slips with single-layer cells was prepared.The concentration of beta-endorphin in the culture was determined by radio-immunoassay.The total RNA was extracted from cells and mRNA from fusion genes was assayed by RT-PCR.Cells on cover slips were subjected to immunofluorescence staining.Results: RT-PCR showed that the fusion genes were expressed in NIH3T3 cells;the expression of beta-endorphin was mainly in the cytoplasm of NIH3T3 cells.The concentrations of beta-endorphin in the supernatants 48 h after transfection with pcDNA3.1-hEP and pcDNA3.1-mEP were(280.33?24.16) pg/ml and(191.04?7.96) pg/ml(P