Objective To study the effect of misoprostol combined with psychological intervention on the patients with induced abortion.Methods 200 cases with intrauterine pregnancy in the first affiliated hospital of Wenzhou medical university from March 2015 to August 2016 were selected as the research object in this study.They were randomly divided into the control group and the experimental group, 100 cases in each group.After using iodophor to rinse the vagina, 600ug misoprostol were placed in posterior vaginal fornix in the experimental group 2 hours before operation.Before the operation, the nursing staff should inform the patient of the relevant surgical attention points, eliminate the negative emotions such as anxiety and anxiety, and improve the treatment compliance.During the operation, the nursing staff should pay attention to the psychological state of the patients.The control group were given lidocaine locally.The clinical efficacy in the groups were compared.Results After treatment, the analgesia obvious effective rate in the experimental group was 38%, the analgesia effective rate was 52%.The analgesia obvious effective rate in the control group was 16%, the analgesia effective rate was 49%.The analgesic effect in the experimental group was significantly higher than that in the control group, with statistical significance (P<0.05).After treatment, the effective rate of cervical dilation in the experimental group was 45%, the effective rate was 66%.The degree of cervical dilatation in the experimental group was significantly better than that in the control group.The amount of intraoperative bleeding was significantly less than that in the control group, with statistical significance (P<0.05).Conclusion Misoprostol combined with psychological intervention can improve the clinical effect of artificial abortion to a large extent, relieve the pain of patients and shorten the operation time.

Objective To observe the rehabilitation of Six Healing Sounds on MCI of the oldaged persons.Methods All patients were randomly divided into Six Healing Sounds exercise group and control group using random number table.MOCA,and MMSE were adopted for evaluating the efficacy of the subjects before and six months after the practice.Nerve-physiology information workstation was setup to record the changes of cerebral electric powder of both groups before and 6 months after the treatment.The data was further analyzed by intelligent cerebral powder analyzing system.Results ①After six months practice,total score of MOCA (25.18 ± 5.07) and MMSE (26.03 ± 2.63) increased significantly after the treatment in the exercise group,which showed significant difference than the control group [MOCA (22.83 ± 4.95) and MMSE (24.86± 2.98)].MOCA increased significantly in attention (5.04 ± 0.90),delay memory (3.02 ± 0.89),verbal(2.30±0.57) sub-item scores after the treatment in the exercise group,showed significant differences than the control group [attention (4.28 ± 1.08),delay memory (2.33 ± 0.95),verbal (2.13 ± 0.51)] ; MMSE also demonstrated a significant increase a in split-second memory(2.35 ±0.43),delay memory(2.42±0.48),verbal (8.49± 0.54) sub-item scores after the treatment in the exercise group,showed significant differences than the control group [split-second memory (2.15 ± 0.34),delay memory (2.14± 0.83),verba (8.13 ± 0.70)].②Frequency changes:the change of δ (0.0848± 0.0373) μ v 2 was decreased than before in the exercise group after 6 months; the change of α1 (0.3753± 0.1470) μ v2was increased than before in the exercise group after 6 months; the change ofδ and θ (0.2266±0.0953)μ v2 had significant difference after the treatment between the two group [control group:δ (0.1095 ± 0.0520) μ v2 θ (0.2589 ± 0.0862) μ v 2and α1 (0.3160 ±0.1540)μ v 2].③Space changes:in the ratio of (δ + θ) / (α1+α2+β1+β2):F4(0.5115±0.4262)、O 1 (0.3365 ± 0.2436)、O2 (0.3536± 0.2671)、T4 (0.4581 ± 0.3798)、T5 (0.3198± 0.3608)、Fp (10.8233 ±0.6857) 、Fp2 (0.7710± 0.6352)、C3 (0.3702 ± 0.3910)、C4 (0.3896 ± 0.3523)、P4 (0.3523 ± 0.2333)、T6 (0.2972± 0.4276)were decreased than before in the exercise group after 6 months; C3、C4、P4、T4、T5 、T6、Fpl had significant difference after the treatment between the two group[control group:C3 (0.5706±0.4944)、C4 (0.5780 ± 0.4996)、P4 (0.4835 ± 0.3641)、T4 (0.6294 ± 0.4186)、T5 (0.4882 ± 0.4000)、T6 (0.4879±0.4131)、Fp1 (1.0125±0.7476)].Conclusion Six Healing Sounds had rehabilitation effects on MCI of the old-aged persons.

This study was aimed to explore the mechanism of electroacupuncture (EA) on improvement of Parkinson’s disease (PD). A total of 40 SD male rats were randomly divided into 4 groups, which were the normal group, sham-operation group, model group, and EA group (n=10). The 6-hydroxydopamine (6-OHDA) was used to prepare PD rat rotational model. The 0.2% Vitamin C of physiological saline was injected in the sham-operation group. EA on GV16-Fengfuand LR3-Taichongwas given for 30 min in the EA group after the model was successfully established, once a day, 7 days a treatment course. The treatment was given for 2 courses. Behavioral test was used to detect PD rat rotational behavior changes. Glu concentration in the striatum was detected by HPLC assay. The expression levels of GS and PAG were detected with western blot. The results showed that there were significant differences in the rotational behavior before and after treatment in the EA group (P < 0.01). Compared with the normal group and sham-operation group, Glu concentration significantly increased, GS expression obviously decreased, and PAG expression significantly increased in the model group (P < 0.01). Compared with the model group, Glu concentration obviously decreased (P < 0.05), GS expression significantly increased, and PAG significantly decreased in the EA group (P < 0.01). It was concluded that the protective effect of EA in PD may be associated with EA improving the expression of GS, reducing the expression of PAG, and relieving the cytotoxicity induced by Glu in the brain.

Objective:To explore the effect of evidence-based quality control circle activities in improving the implementation rate of enhanced recovery after surgery in gynecological tumor surgery.Methods:Gynecological tumor surgery patients admitted to the First Affiliated Hospital of Wenzhou Medical University were selected as the research object from January to March 2018 and September to December 2018, respectively. A total of 80 patients before the quality control circle activities from January to March 2018 were used as the control group, and the conventional enhanced recovery program was adopted. A total of 78 patients after the quality control circle activities from September to December 2018 were taken as the observation group, and the enhanced recovery program was implemented based on the evidence-based quality control circle. The implementation rate of enhanced recovery after surgery, postoperative rehabilitation indicators, and hospitalization satisfaction of the two groups of patients were compared.Results:The total implementation rate of the 8 enhanced recovery after surgery in observation group was 86.9% (542/624) , which was higher than 62.2% (398/640) of control group. The total incidence of complications in observation group was 6.4% (5/78) , which was lower than 22.5% (18/80) in control group. The early ambulation time, exhaust time, and hospitalization time of observation group were shorter than those of control group, and the hospitalization satisfaction of observation group was higher than that of control group. The above differences were statistically significant ( P<0.05) . Conclusions:Evidence-based quality control circle activities can standardize the practice standards for enhanced recovery, facilitate the development of enhanced recovery after surgery for gynecological tumor surgery, and promote postoperative recovery of patients.

Objective:To explore the effect of preoperative rehabilitation management based on enhanced recovery after surgery (ERAS) in patients with gynecological tumor surgery.Methods:Using the convenience sampling method, 80 patients with gynecological tumor surgery who were admitted to the First Affiliated Hospital of Wenzhou Medical University from January to May 2020 were selected as the control group, and 82 patients who underwent gynecological tumor surgery from January to May 2021 were selected as the observation group. The control group was treated with conventional ERAS program, and the observation group received preoperative rehabilitation management on the basis of the control group. The postoperative rehabilitation indicators (time of getting out of bed, time of exhausting and defecation, occurrence of complications) , depression and satisfaction with medical treatment were compared between the two groups.Results:The time of first getting out of bed, the time of exhausting, the time of defecation and the total complication occurrence of the observation group were lower than those of the control group, and the differences were statistically significant ( P<0.05) . The postoperative depression of the observation group was lighter than that of the control group, and the difference was statistically significant ( P<0.05) . There was no significant difference in patient satisfaction between the two groups ( P>0.05) . Conclusions:The implementation of ERAS-based preoperative rehabilitation management for gynecological tumor surgery patients is conducive to promoting postoperative recovery and reducing depression in patients.

Objective:To explore the clinical characteristics and genetic etiology of a child with Spondyloocular syndrome (SOS) in order to enhance the awareness and understanding of this disease.Methods:A 3.5-year-old boy with SOS who had presented at the Department of Medical Genetics of Hunan Children′s Hospital on August 10, 2023 due to the repeated fractures for over 2 years and after binocular cataract surgery was selected as the study subject. Clinical data of his pedigree were collected, and peripheral venous blood samples were collected for the extraction of genomic DNA and subjected to trio-whole exome sequencing. Candidate variants were verified by Sanger sequencing and analyzed with bioinformatic software. This study was approved by the Medical Ethics Committee of Hunan Children′s Hospital (No. KYSQ2022-263).Results:The child had manifested repeated fractures, bilateral bowed femur, osteoporosis, cataract, atrial septal defect, and developmental delay. Ultrasonography has revealed fetal edema, peritoneal effusion, pleural effusion and polyhydramnios. Trio-whole exome sequencing and Sanger sequencing revealed that he has harbored compound heterozygous variants of the XYLT2 gene, namely c. 1103_1104delAG (p.Gln368Argfs*8) and c. 1238_1253delinsA (p.Val413_Pro418delinsGlu), which were inherited from his phenotypically normal father and mother, respectively. Neither variant was reported previously. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG) and recommendations from the Clinical Genome Resource (ClinGen), the c. 1103_1104delAG was predicted as a pathogenic variant (PVS1+ PM2_Supporting+ PP4), whilst the c.1238_1253delinsA was predicted as a likely pathogenic variant (PM4+ PM3+ PM2_Supporting+ PP4). Conclusion:The c. 1103_1104delAG and c. 1238_1253delinsA compound heterozygous variants of the XYLT2 gene probably underlay the pathogenesis in this child. Above finding has enriched the phenotypic and mutational spectrum of SOS, and provided a basis for the clinical diagnosis, treatment, prognosis assessment and genetic counseling for this pedigree.

A 13-year and 6-month-old girl attended the Hunan Children's Hospital due to delayed menarche. The laboratory test results indicated increased follicle-stimulating hormone and luteinizing hormone, decreased anti-Mullerian hormone, and pelvic ultrasound showed a cord-like uterus and absence of bilateral ovaries. Her 11-year and 5-month-old younger sister had the same laboratory and imaging findings, and both girls were diagnosed with primary ovarian insufficiency. Whole exome sequencing and Sanger sequencing confirmed that the proband and her sister carried heterozygous variants of HROB gene c.718C>T (p.Arg240*) and c.1351C>T (p.Arg451*), which were inherited from their parents respectively and consistent with autosomal recessive inheritance. Oral estradiol valerate at an initial dose of 0.125 mg/d was given to the proband, and the secondary sexual characteristics began to develop after 6 months.
Humans ; Female ; Child ; Infant ; Primary Ovarian Insufficiency/genetics* ; Luteinizing Hormone ; Estradiol