Brain slice technique has been widely applied in the field of neuroscience.This article reviews the application of brain slice in anti-cerebral ischemia pharmacology research on electrophysiology,synaptic plasticity,pathomorphology,neurotransmitters and in the field of Chinese medicine.

OBJECTIVE To evaluate the exposing risks among dental staff and improve their concerns for prevention. METHODS By checking and evaluating the various risk factors,including HIV,HBV,HCV,SARS infectious antigens,and mercury contamination in our hospital,we found and formulated effective ways for preventing occupational disease. RESULTS Most dental staff in our hospital effectively decreased the professional exposing risks by practising preventive strategies. CONCLUSIONS Accurate evaluation and practical preventive strategies are key factors to reduce the professional exposing risks for dental staff.

Objective: To investigate the prevalence of colorectal cancer in Yongjia County, Zhejiang Province from 2020 to 2021, so as to provide insights into colorectal cancer control. Methods: Residents registered in Yongjia County at ages of 50 to 74 years were sampled, and positive populations were screened using Zhejiang Provincial Questionnaires for Assessment of Risk of Colorectal Cancer Screening among High-risk Populations and fecal occult blood test, and those positive for questionnaires or fecal occult blood test served as a positive screening. Residents with a positive screening underwent colonoscopy, and the detection of high-risk lesions (colorectal cancer and pericancer lesions) was analyzed among those positive for questionnaires, fecal occult blood test and screening. Results: Totally 102 504 residents were tested, and 46 138 (45.01%) residents completed questionnaire surveys and fecal occult blood tests. The rate of positive screening was 21.10%, and the rate of positive questionnaire surveys was 7.13%, while the positive rate of fecal occult blood test was 15.16%. The rate of positive screening was significantly higher in men than in women (27.06% vs. 17.14%; (χ2=654.747, P<0.001), and there was an age-specific proportion of positive screening (χ2=418.264, P<0.001), with high prevalence seen in residents at ages of 65 to 69 years (26.43%) and 70 to 74 years (24.98%). Colonoscopy was performed among 2 966 residents, with a compliance rate of 30.47%, and colonoscopy detected 348 residents with high-risk lesions, including 317 cases with pericancer lesions and 31 cases with colorectal cancer, with 11.73% detection of high-risk lesions and 1.05% detection of colorectal cancer. The prevalence of high-risk lesions was significantly higher in men with positive screening than in women with positive screening (15.77% vs. 7.59%; χ2=47.915, P<0.001), and there was a significant difference in the detection of high-risk lesions among residents with positive screening at different age groups (χ2=25.909, P<0.001), with a high detection in residents at ages of 65 to 69 years (14.32%) and 70 to 74 years (15.49%). Conclusions The prevalence of high-risk lesions of colorectal cancer is 11.73% among residents living in Yongjia County at ages of 50 to 74 years, and men and the elderly at ages of 65 years and older are high-risk populations for colorectal cancer. Improving the awareness of colorectal cancer prevention knowledge and the compliance of colorectal cancer screening is recommended.

Objective To analyze the characteristics of iatrogenic urerteral injury and summarize the experiences in prevention,diagnosis and treatment of iatrogenic urerteral injury. MethodsA review was made on the injurycauses,the injury locations,the treatment time,the methods of surgical procedures and the results of treatment in 17 patients with iatrogenic ureteral injury treated surgically from 1997 to 2003. Results Of 17 cases of iatrogenic ureteral injuries,gynecological,general surgical and urological procedures resulted in ureteral injuries in 12 cases (71%),four (24%) and one (6%),respectively. Of all the injuries,65% (11/17) appeared in the lower part of the ureter,18% (3/17) in the middle part of the ureter and 18% (3/17) in the upper part of the ureter. The main injury causes were ligation,partial ligation,complete transection and perforation,accounting for 29% (5/17),41% (7/17),24% (4/17) and 6% (1/17),respectively. Four cases were found during operation,nine at days 2-11 after operation and four were treated 3-6 months after injury. Treatment methods included end-to-end ureteral anastomosis in seven cases,ureteroneocystostomy in three,ureteral lithotomy in one,pure ureteral lysis in three and post-lysis double-J tube insertion in three. All patients were cured. The follow-up ranging from six months to three years showed no patients suffering from urinary tract infection,hydronephrosis or atrophy. Conclusions The location and type of injury determine the type of surgical repair. A thorough knowledge of pelvic anatomy and mastering the basic steps of diagnosis and treatment are critical for prevention and management of the iatrogenic urerteral injury.

Objective To assess the efficacy and safety of minimally invasive percutaneous nephrolithotomy(mPCNL)in the treatment of upper urinary tract calculus. Methods The clinical data of 368 cases of upper urinary tract calculus from 2002 to 2006, which underwent mPCNL, were retrospectively analyzed. Among 368 cases analyzed, there were 116 cases with proximal ureteral cal-culus;190 cases of nonstaghorn kidney stones, 62 cases of staghorn stones. Results There were 344 cases(93.5%)treated with one-stage operation, 24 cases(6.5%) with two-stage. Single channel was used in 856 cases(96.70%), two-channel in 12 cases(3.3%). Complete stone clearance was a-chieved in 337 kidneys, giving an overall clearance rate of 91.6%. The average operative time was 73 min. The duration of hospital stay was 4-8 d with an average of 6 d in one-stage and complete clear-ance patients. Postoperative urinary tract infection was seen in 23 patients(6.2 %). Five(1.4 %) pa-tients required blood transfusion after operation. Two patients with severe bleeding were treated with blood transfusion and super-selective arterial embolization. Conclusion mPCNL has definite efficacy in the treatment of upper urinary tract calculus with little suffering and short recovery time.

Objective To explore the effects of ureteral stent on renal pelvic pressure and other urodynamic parameters. Methods Forty-one patients, 28 males and 13 females, with unilateral renal calculi and/or ureteral calculi were recruited in this study. The mean patient age was 47 years old (ranging from 20 to 72 years old). All cases were placed a 4.7 F ureteral stent and 16 F nephrostomy tube after minimal invasive pereutaneona nephrolithotomy (MPCNL). There was no hydronephrosis and residual crushed stone in the ureter after MPCNL in all cases. Renal pelvic pressure, intra-abdo minal pressure, detrusor pressure, bladder pressure changes during the filling and voiding phases with intravesical perfusion flow rate of 40 ml/min were recorded and analyzed. Results At the baseline, IPP0, IAP0, DP0 and BP0 were (33.1±17.0)cm H2O, (27.5±7.0)cm H2O, (3.3±2.9)cm H2O and (30. 9±7.2)cm H2O, respectively; At the maximum cystometric capacity during the filling phase, IPPvol, IAPvol Dpvol and Bpvol were (39.4±67. 3)cm H2O, (31.1±7.3)cm H2O, (10.7±6. 6) cm H2O and (41.6±10.3)cm H2O, respectively; At the maximum bladder pressure during the voiding phase, IPPmax, IAPmax Dpmax and Bpmax were (65.7±17.0)cm H2O, (33.7±9. 7)cm H2O, (41.9±7.8)cm H2O and (75.0±12. 8)cm H2O, respectively;There were statistical significance comparing between any of IPP0, IPPvol and IPPmax(P<0. 01). 27% (11/41)patients were with the pain in kidney area at voiding IPPmax (87.1±14.6) cm H2O, which was significantly higher than IPPmax (57.8±9.5)cm H2O of asyrnptomatic group (30 patients)(P<0. 01). In all cases, the renal pelvic pressure was higher than 40 cm H2O during the voiding phase. Conclusions Renal pelvic pressure increases during the filling phase after placing the ureteral stent, especially during the voiding phase. As renal function will be damaged by the high renal pelvic pressure, we should decrease the utilization of ureteral stent if possible. It is encouraged to remove the ureteral stent as early as possible.

BACKGROUND:There is a great dispute on the choice of repair materials for ossicular chain damage.
OBJECTIVE:To explore the effects of new hydroxyapatite-bone morphogenetic protein ossicular prosthesis versus al ogeneic cartilage ossicular prosthesis in patients with ossicular chain damage.
METHODS:Sixty patients with chronic otitis media were equal y assigned into a control group and a test group. Two groups of patients underwent tympanoplasty and ossicular chain reconstruction with al ogeneic cartilage ossicular prosthesis or hydroxyapatite-bone morphogenetic protein ossicular prosthesis, respectively. Twelve months after surgery, fol ow-up results were compared between two groups.
RESULTS AND CONCLUSION:Air conduction value and air-bone gap value were both improved significantly in the two groups after surgery (P<0.05), and especial y, the postoperative air conduction value was better in the test group than the control group (P<0.05). Implant detachment was found in three cases of the control group, whereas did not occur in the test group. Improved hearing was found in 28 cases in the test group with an improvement rate of 93%and 25 cases in the control group with an improvement rate of 83%. There was a significant difference in the hearing improvement between the two groups (P<0.05). Overal , two kinds of prostheses can share similar effects on the ossicular chain repair.
Subject headings:Tympanoplasty;Otitis Media;Bone Morphogenetic Proteins;Tissue Engineering

Objective To investigate the neuroprotective effect and possible mechanism of Compound Porcine Cerebroside and Ganglio-side Injection (CPCGI) on cerebral ischemia-reperfusion injury in rats. Methods Healthy adult male Sprague-Dawley rats were divided into sham group (n=10), model group (n=10), CPCGI low dosage group (n=10) and high dosage group (n=10), and control group (Ginkgo biloba extract, n=10). All the rats was subjected to middle cerebral artery occlusion (MCAO) for two hours and reperfusion except sham group, and received treatment for fourteen days once reperfusion started. They were tested with modified Neurological Severity Score one, three, seven and fourteen days after MCAO, and adhesive-removal test and beam-walking test fourteen days after MCAO. The expression of Beclin1, PINK1 and Parkin were detected with Western blotting. Results Compared with the model group, the Neurological Severity Score reduced (P<0.05) and the time crossing the beam reduced (P<0.01) in all the medical groups fourteen days after MCAO, and the time removing the adhesive paper reduced in the CPCGI groups (P<0.01). The expression of Beclin1 and Parkin decreased and the PINK1 level increased in the model group (P<0.01), and it was reversed in all the CPCGI groups (P<0.05). Conclusion CPCGI could relieve the cerebral ischemia-re-perfusion injury in rats through the regulation in mitophagy.

OBJECTIVETo study the direct effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury and elucidate their therapeutic mechanism on ischemic heart diseases.

METHODCultured cardiomyocytes of neonatal rats were subjected to anoxia-reoxia injury which simulated the ischemia-reperfusion injury in vivo, and hawthorn leaf procyanidins were applied. The therapeutic effect was valued by LDH leakage and MTT test. For a further mechanism study, contents of MDA and activities of SOD in cardiomyocytes were measured.

RESULTHawthorn leaf procyanidins in 24-60 mg x L(-1) significantly and dose-dependently inhibited LDH leakage (compared with the model group, all P<0.01 ) and cell viability decrease (compared with model group, 24-48 mg x L(-1) groups all P<0.05; 60 mg x L(-1) group, P<0.01) in cardiomyocytes induced by anoxia-reoxia injury. Furthermore, hawthorn leaf procyanidins in 24-60 mg x L(-1) significantly inhibited the increase of MDA content (compared with the model group, all P<0.01) and the decreased of SOD activity (compared with the model group, 24 mg x L(-1) group, P<0.05; other groups all P<0.01) in cardiomyocytes undergoing anoxia-reoxia injury.

CONCLUSIONHawthorn leaf procyanidins have a significant therapeutic effect on the simulated ischemia-reperfusion injury of cultured neonatal rat cardiomyocytes, which may relate to their anti-oxidation effects. And the direct protective effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury may be one of the key mechanisms of its therapeutic effect on ischemic heart diseases.

Animals ; Biflavonoids ; pharmacology ; Catechin ; pharmacology ; Cell Survival ; drug effects ; Crataegus ; chemistry ; Female ; Hypoxia ; metabolism ; pathology ; L-Lactate Dehydrogenase ; metabolism ; Malondialdehyde ; metabolism ; Myocytes, Cardiac ; drug effects ; metabolism ; Plant Leaves ; chemistry ; Proanthocyanidins ; pharmacology ; Rats ; Reperfusion Injury ; metabolism ; pathology ; prevention & control ; Superoxide Dismutase ; metabolism

Objective To prepare the human papillomavirus (HPV) 16 peptide vaccine and explore the effect in vitro and in vivo. Methods (1) Prediction of the major histocompatibility complex (MHC) class I restricted T cell epitopes by bioinformatics target at transporter associated with antigen processing (TAP) and named by E7Pa, E7Pb, E7Pc separately. (2)In vivo, the C57BL/6 mice were divided into five groups with same amounts randomly after loading with TC-1 cells (HPV 16 positive tumor cells from C57BL/6 mouse), named as E7Pa + CpG,E7Pb + CpG,E7Pc + CpG (as experiment groups, and added 50 μg/ml E7Pa, E7Pb, E7Pc, respectively), CpG(as positive control group and added Con A with 12 mg/L final concentration) and blank control group (without any treatment). The T cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay at different time points;the lactate dehydrogenase (LDH) delivery method was used to test the cytolytie T lymphocyte (CTL) activity of mouse splenic lymphocyte in different ratio of effector cells and target cells (E:T);the related cytokines in tumor tissue and mouse peripheral blood were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The tumor volumes were measured to contrast the therapeutic effect in different groups. Results (1) Three peptide named E7Pa, E7Pb, E7Pc were successfully preparated which had high affinity and specificity. (2) After vaccination of 24, 48, 72,96 hours, MTT results shown that the proliferation rate in E7Pa + CpG group were(131±32)%, (302±15)%, (552±28)%, (731±24)% individually, which were much higher than those in blank control [(72± 15) %, (120 ± 57) %, (176 ±41)%, (288±29)% ;P<0.01], and the other groups i. e. E7Pb + CpG,E7Pc +CpG and CpG groups all proliferated much higher than those in blank control group with statistic signification (P<0. 05), but there was no significant difference between groups(P>0.05);the LDH delivery assay showed that when the ratio of E:T was 100:1, the activity of CTL in the E7Pa + CpG group was most powerful than the other groups with statistic signification (P<0. 01). Meanwhile, the ratio of E:T was concentration-dependent. Compared E7Pb + CpG, E7Pc + CpG or CpG groups with blank control group, there were significantly difference(P<0. 05) ,while there was no significant difference between groups(P >0. 05). The mRNA levels of interferon γ (IFN-γ), interleukin-2 (IL-2) in tumor tissue and peripheral blood in E7Pa + CpG group were significantly higher than those in blank control group (P<0. 01), which was the similar results when compared E7Pb + CpG, E7Pc + CpG or CpG groups with control group (P < 0. 05), and without significant difference between groups(P > 0. 05). The tumor volumes were suppressed obviously in all the experiment groups, especially at the 60th days, the volumes in ETPa + CpG group were much smaller than that in blank control group with statistic signification (P < 0. 01),which was the similar results that E7Pb + CpG, E7Pc + CpG or CpG groups had difference than blank control group with statistic signification (P < 0. 05), and without significant difference between groups(P >0. 05). Conclusion The HPV16 E7 peptide target at TAP combination with CpG as a vaccine could treat effectively the HPV16 E7 positive tumor in experiment.