OBJECTIVE To analyze occupational risk factors onto Central Sterile Supply Department(CSSD) staff caused by actual working conditions and provide correspondingly protective measures.METHODS Take actions,such as circulating the air inside working site,eliminating contamination which brought into by biological and chemical methods,and avoiding physical fatigue etc,which summarized by practical study and analysis on the(various) factors during daily work routine,e.g.physical factor,chemical factor,biological factor and psychological factor.RESULTS All methods mentioned in this article could improve self-protection awareness of CSSD staff and(enhance) the physical condition of staff as well as reduce pollution on environment.CONCLUSIONS For preventing the threats of occupational risk factors,it′s critical to reduce or eliminate the negative factors in CSSD.