Objective To observe the effect of Lipo PGE1 and Mecobalamine and Ginkgo-dipyidamolun in the treatment of dia-betic peripheral neuropathy. Methods 60 Patients with diabetic peripheral neuropathy were divided randomly into two groups; the control group (30 cases) and the treatment group (30 cases). The patients of the treatment group were given with Lipo PGE1 100 ?g dissolved in 0.9% normal saline solution 100ml once a day intravenously, and given mecobalamine 500 ?g once a day intramuscular, and given Ginko-dipyidamolum injection 20ml dissolved in 0.9% normal saline solution 250ml once a day intravenously; and in the control group were only given with mecobalamine 500?g once a day intramuscular. Nerve conduction velocity (MNCV and SNCV) in two groups of patients was observed after 14 days treatment. Results After treatment for 14 days, the subjective symptoms and signs were significantly improved with a total effective rate of 86.7% in the treatment group versus 56.7% in the control group. Compared with the control group, the total effective rates of treatment group was significantly higher (P

Objective To develop a loop-mediated isothermal amplification method for rapid diag-nosing Vibrio cholerae O139 in inspection department and small-scale laboratory. Methods Four primers (2 inner primer and 2 outer primer) for the LAMP test were designed by targeting the wbfR gene of Vibrio chol-erae O139, and the reaction condition and reaction system of LAMP were optimized. Thirty Vibrio cholerae O139, 13 Vibrio cholerae reference strains, 10 O1 biotype Vibrio cholera* and 32 other enterobacterias were analyzed and the LAMP results were determined by visual inspection or electrophoretie analysis . Results All of the Vibrio cholerae O139's amplification products were observed as green by visual inspection and had a ladder-like pattern on the gel, but O1 biotype Vibrio cholera* and other enterobacteria's products were dis-played as orange by visual examination and had no ladder-like pattern on the gel. In addition, the reaction time of the LAMP method was only 1.5 h and the detection limit of this assay was 63 CFU/reaction. Con-clnsion LAMP assay targeting the wbfR gene of Vibrio cholera* O139 is rapid, specific, and sensitive for the detection of Vibrio cholerae O139. This method not only reduced the dependence of complicated equip-ment but also had a potential for wider use in inspection department, small-scale laboratory, emergency mo-tor vehicles and field survey.

Background:There is growing evidence that cytokines are the key elements in the initiation,evolution,and,ultimately,the resolution of inflammatory bowel disease (IBD).Ahns:To investigate the differences of cytokine expressions in intestinal mucosa between IBD patients and normal controls.Methods:Thirty cases of colonoscopic or enteroscopic biopsied mucosal tissue were collected from 7 normal subjects,12 ulcerative colitis (UC),and 11 definite or suspicious Crohn's disease (CD) during Jan.2014 to Dec.2014 at Shanghai East Hospital South Branch.Using the suspension chip technology,expressions of 27 cytokines in mucosal tissues were detected.Results:Most of the cytokines in intestinal mucosa of patients with IBD were decreased as compared with the normal controls,whereas the expressions of interleukin-5 (IL-5) and IL-7 were increased.Among the 27 cytokines,statistically significant differences were seen in expressions of IL-1β,IL-2,IL-15,interferon-γ-induced protein-10 (IP-10),monocyte chemoattractant protein-1 (MCP-1),macrophage inflammatory protein-1α (MIP-1α) and MIP-1β between UC,CD and normal controls (P ＜ 0.05);in addition,significant differences were seen in expressions of IL-8 and platelet-derived growth factor-BB (PDGF-BB) between CD and normal controls (P ＜ 0.05).No significant differences were observed in expressions of all 27 cytokines between UC and CD (P ＞ 0.05),as well as between active and remission stages of IBD (P ＞ 0.05).Conclusions:Expressions of IL-1β,IL-2,IL-15,IP-10,MCP-1,MIP-1α and MIP-1β are significantly down-regulated in intestinal mucosa of IBD patients.It may help to further explore the role of cytokines in IBD.