OBJECTIVE: To discuss a mental disorder case caused by levofloxacin and theophylline. METHODS: Based on one case, the characteristics of interaction and adverse reaction of levofloxacin and theophylline were summarized from the perspe ctive of clinical pharmacists to put forward advice for rational use of drug. RESULTS:Levofloxacin and theophylline used unsuitable can cause mental disorder. CONCLUSION: The dosage should be adjusted correctly when use levofloxacin and theophylline. It should be emphasized that the clinical pharmacists play important role in education and instruction for medication. Patient should be told the importence of rational drug use, in order to avoid ADR.

Biliary duct injury can arise severe complications,but the proper management can improve patients'prognosis and living quality.So we reviewed the current relevant references and hope it can do some help for clinicians.

Objective To explore the clinical application of omeprazole combined with psychological nursing intervention in the treatment of peptic ulcer. Methods 80 patients with peptic ulcer treated in our hospital from April 2014 to October 2016 were selected, and randomly divided into control group and experimental group, 40 patients in each group. The control group was treated with omeprazole, amoxicillin and tinidazole, and the experimental group was treated with psychological nursing intervention based on the treatment of the control group. Both groups were treated for four weeks in a row. The clinical efficacy and the recurrence rate after treatment were followed up to half a year after treatment in two groups were compared. Results After four weeks of treatment, the total effective rate in experimental group was 92.5％, which was significantly higher than that in the control group (P<0.05). After six months of follow-up, the recurrence rate of patients in the experimental group was significantly lower than that in the control group, and the difference was statistically significant (P<0.05). Conclusion Patients with peptic ulcer treated with omeprazole combined with psychological nursing intervention, the effect is significant, relieve the patient's clinical symptoms, effectively improve the clinical cure rate of peptic ulcer recurrence rate is low, high security, is worth popularization and application in clinic.

Objective To investigate the effects of SLB elements presented at 5' UTR of dengue virus genome on viral translation and RNA replication.Methods The 5 end of RNA secondary structures of dengue virus genome were predicted using mfold 3.2,and three mutants with different modifications in SLB elements were designed:SLB1,part deletion of the 5' UAR sequence(82-87nt);SLB2,mutation of the sequence on stem(Mut 77G/C);SLB3,mutation of the sequence on stem(M77-78AG/GA),respectively.The mutants described above were constructed by OL-PCR based on DEN-R.luc2A-RP,respectively.Replicon RNAs corresponding to DEN-R.luc2A-RP,DEN-R.luc2AGDD-RP and the 3 mutants mentioned above were in vitro transcribed and equal amounts of RNA were transfected into BHK cells with Lipofectamine 2000.After RNA transfection,the replicons were detected and characterized by RT-PCR,IFA,Renilla Luciferase assay system and real time RT-PCR,respectively.Results It was shown that SLB1 mutant did not significantly affect the translation of the input RNA,but seriously compromised RNA synthesis;SLB2 mutant did not significantly affect the translation of the input RNA either,but its RNA replication was abolished;both the translation and replication of SLB3 mutant were abolished.Conclusion Both the nucleotide sequence and the RNA secondary structure of the second loop and short stem of SLB element are highly conserved.SLB element may play an essential role on viral translation and RNA replication.The findings of present study may set a foundation for elucidating the role of SLB during viral translation and replication.

Objective:In order to study of diagnose anaphylactic shock in forensic field,a new in vitro testing sysytem were established.Methods:The RBL 2H3 were cultured and sensitized,the release of histamine and stained for Annexin V were testing by fluorescent spectrometer and flow cytometry respectively.Results:The degree of RBL 2H3 degranulation after sensitived serum treated was higher than that after control serum treated,and the degranulation degree increased dose dependently.Conclusion:RBL 2H3 are helpful to diagnose anaphylactic shock,and the release of histamine and the positive staining for Annexin V may be the ideal indices.

Objective To explore the radiosensitivity ofβ-elemene on Colo320 cells transplanted tumor in nude mice and its molecular mechanisms.Methods Colo320 cells transplanted tumor model was established by cell suspension inoculation in nude mice.Then the transplanted mice with 0.8-1.0 cm3 tumor were randomly divided into 4 groups:control,β-elemene (40 mg/kg),radiation (4 Gy),andβ-elemene (40 mg/kg)+ radiation (4 Gy) groups,with four to five mice in each.Tumor weight and morphology were observed in each group.In addition,the apoptosis of tumor cells was measured by TUNEL and the expression of Fas gene was detected by immunohistochemistry.Results The nude mice transplanted tumor model was successfully established.Compared with that in control and simple radiation groups,tumor weight was significantly decreased inβ-elemene combined with irradiation group (P <0.05).At the same time,the apoptosis rate and the expression of Fas gene in tumor cells were significantly increased (P <0 .0 5 )inβ-elemene combined with irradiation group compared with control and simple radiation groups. Conclusion β-elemene could enhance the radiosensitivity of Colo320 cells transplanted tumor in nude mice probably by inducing Fas-mediated apoptosis of tumor cells.

AIM: To explore the role of activation and apoptosis of splenic lymphocytes in the development of viral myocarditis (VMC). METHODS: Apotosis and MHC II antigen of splenic lymphocytes were detected in the VMC group (VMC, death, 8 cases) and control group (non-cardiac death, 4 cases) with TUNEL and immunohistochemistry methods.RESULTS: Compared with the control group, the increased expression of MHC II antigen and apoptosis were found in the splenic lymphocytes in the VMC group. CONCLUSION: These results suggest that the abnormality of the apoptosis/activation of splenic lymphocytes may be involved in the pathogenesis of VMC.

AIM: To study the changes of plasma atrial natriuretic peptide(ANP), endothelin-1 (ET-1) and serum cardiac troponin I(cTnI) levels and the effects of ANP and ET-1 on myocardium in crush injury rats. METHODS: Crush injury was produced in SD rats,the serum levels of cardiac enzyme and cardiac troponin I,plasma levels of ANP and ET-1 were studied by automated biochemical analyzer,automated chemiluminescence assay and radioimmunoassay. RESULTS: The plasma ET-1 level was much lower ,the levels of plasma ANP and serum cardiac enzyme and cTnI were much higher after crush injury than those of the control group( P

OBJECTIVE:To investigate the effect of aqueous extraction of Lonicerae Flos (SYHW) on anti-influenza A virus H1N1 (H1N1 virus) in vitro. METHODS:Using Madin-Darby canine kid ney (MDCK) cells cultured in vitro by H1N1 virus, half of the tissue culture infection dose(TCID50)was calculated. Culturing MDCK cells for 24 h with different mass concentrations of SYHW,the maximum non-toxic concentration was investigated. And then test was divided into normal cell group,virus control group,SYHW preventive administration group,therapeutic administration group and direct killing group (given SYHW of maxi-mum non-toxic concentration,infecting cells by 100 TCID50 H1N1 virus),and antiviral effective rate (ER) of SYHW was deter-mined. Test was divided into normal cell group,virus control group,SYHW therapeutic group and direct killing group (the same administration and infection as above),changes of cell proliferation index (PI) and cell apoptosis rate were respectively deter-mined. RESULTS:100 TCID50 of H1N1 virus was 1.26×10-7,and the maximum non-toxic concentration of SYHW on MDCK cells was 50 μg/mL(cell survival rate was 91.3%). ERs of preventive administration group,therapeutic administration group and direct killing group were 0,80.3% and 52.7%,respectively. Compared with normal cell group,PI value in virus control group was sig-nificantly reduced (P<0.05),early and late apoptotic rates were significantly increased (P<0.05). Compared with virus control group,PI value in directly killing group was significantly increased(P<0.05),and early apoptotic rate was significantly reduced (P<0.05);early apoptotic rates in therapeutic administration group were significantly reduced (P<0.05). CONCLUSIONS:SY-HW shows anti-H1N1 virus effect in vitro,therapeutic administration and directly killing are preferred in antiviral effect.

Objective To evaluate the diagnostic efficiency of deep fungal infection by detecting the serum galactomannan ( GM) and bronchoalveolar lavage fluid ( BALF) GM, serum G test and fungal culture of BALF in patients with suspected invasive fungal infection ( IFI) in lungs.Methods A retrospective analysis was performed of the results of serum /BALF GM test ,serum G test and BALF culture from 148 patients with suspected pulmonary IFI .The indexes involved sensitivity , specificity , positive predictive value , negative predictive value , as well as diagnostic capacity for deep fungal infection with separated or combined tests .Results Among the 148 cases, 48 cases were clinically diagnosed with IFI and the rest were excluded.Among the 48 IFI cases, 3 cases were positive in serum GM test , 25 cases were positive in BALF GM test , 31 cases were positive in G test and 30 cases were positive in fungal culture .The combined detection showed a sensitivity of 91.6%,specificity of 70.0%, positive predictive value of 59.5% and negative predictive value of 94.6%.Conclusion The combination of GM/G tests and fungal culture can significantly improve the clinical diagnostic efficiency of pulmonary IFI .