Objective To discuss the indications and efficacy for treating tibia insertion avulsion fracture of the posterior cruciate ligament(PCL)by the mean of the end of ligament suture via arthroscopy.Methods Twenty-one cases of tibia insertion avulsion fracture of PCL were treated with suture the end of ligament via arthroscopy from June 2007 to June 2009,including 14 males,7 females,with a mean age of 31.5 years(range,13-52 years).The insertion avulsion fragment was fixed to the tibia with two strands of 5Ethibond threads via arthroscopy.Two bone tunnels(2.5 mm in width)were made from the anterior part of the tibia to the posteromedial(at 4:30 toward)and posterolateral(at 7:30 toward)of the tibia bone bed respectively.Then the sutures were pulled out through the two bone tunnels and tied to the door-nail.Reduction and healing of the avulsion fragment,relaxation and mobility degree of knee,and the influence of osteoepiphysis to teenage patients were observed after surgery.KT-2000 value and Lysholm score were calculated.Results All cases were followed up from 10 to 24 months,with an average of 13.5 months.All the fragments were united without displacement in all cases according to X-ray films.One case showed posterior drawer test(+),but the tibia insertion was healed.The knee extension in all cases was unlimited,and 10° to 15° flexion limitation was found in 2 cases.The average range of flexion was 140.5°±3.8°.On average,KT-2000 value was(1.2±0.4)mm,Lysholm score was 95.2±2.7.Conclusion For treating the avulsion fracture of PCL,suture the end of ligament via arthroscopic transfixion can get a reliable fixation and a satisfied function of the knee,especially,which is suitable for the small avulsion fracture fragments,the adolescent patients with premature epiphysis.

Objective To investigate the clinical efficacy of porous calcium phosphate bioceramics (PTCP)for treating stage FicatⅢ non-traumatic osteonecrosis of the femoral head(ONFH)in the patients aged below 60 years old.Methods A total of 41 patients (63 hips)with non-traumatic ONFH in our hospital from March 2006 to July 2009 were collected and divided into two groups.The group A (n=1 6)was performed the core decompression only,while the group B (n=25)was performed the core de-compression plus through the head-neck junction of the femur to opening the window light bulb shape focus debridement and impac-ting bone grafting combined with PTCP implantation.The various clinical evaluation indicators were observed and evaluated.Results No statistically significant differences in PRI were found at each time point in the group A.PRI at postoperative 1 month in the group B was significantly decreased than before operation,hereafter,the stable status was maintained and slightly risen again until postoperative 3 years(P =0.033).The Harris scores at postoperative all time points in the group A were significantly lower than those in the group B.The necrosis areas at postoperative 24 months,3,4,5 years in the group B were significantly decreased com-pared with before operation and lower than those in the group A.Conclusion PTCP implantation has satisfactory effect for the treatment of stage FicatⅢ non-traumatic ONFH in the patients aged below 60 years old.

Anterior cruciate ligament (ACL) has two important functional roles, including biomechanics and proprioceptive sense. A groat propr!oceptive sense is a basis to exactly obtain highly functional exercise. ACL injury may block perception and conduction information and seriously influence motion and daily activities. A complete rehabilitation of ACL function depends on rehabilitation of both mechanical structure and proprioceptive sense. Therefore, reconstruction of joint function following ACL injury is focus on reconstruction of both joint biomechanical stability and proprioceptive sense. This study was designed by distribution and function of proprioceptive sense to analyze the altemation of propdoceptive sense following ACE injury, and to investigate the related factors and ways for rehabilitation of propriocaptive sense following ACL reconstruction.

Objective Human cytomegalovirus (HCMV) can induce infection in multiple organs, including genitouri-nary system, central nervous system and liver. It can even lead to serious defects and death. China, as a high-incidence area of HCMV, need employ early treatment. However, the sensitivity of present HCMV diagnosis is not satisfied. Methods In the present study, we focused on transcription level of HCMV IE and pp67 mRNA using real-time quantitative PCR. Results IE mRNA was positive even in a portion of patients who were regarded as negative by traditional DNA diagnosis method. On the other hand, pp67 mRNA that were normally expressed at the late stage of HCMV infection was 100%posi-tive in the first-visit patients who were regarded as positive using traditional DNA diagnosis method. Conclusion The pres-ent study indicated that mRNA detection based on PCR method was more sensitive compared with DNA detection for assess-ing HCMV virus infection. Finally, different treatment should be performed upon mRNA transcription profile at different in-fection stages.

Objective To investigate the forensic applicability of nuclear factor-kappa B p65 (NF-?B p65) to the diagnosis of early myocardial ischemia in cases of sudden cardiac death. Methods The cases were divided into three groups: the cases with early myocardial ischemia, myocardial infarction and cases used as control. Immunostaining of NF-?B p65 was performed by SP method in cardiac tissues. The intensity of NF-?B p65 was observed under light microscope and analyzed semi-5quantitatively. Results NF-?B p65-positivecardiac cells were detected in cytoplasm and nuclei in the cases with early myocardial ischemia and myocardial infarction, and the intensity of immunoreactivity was approximately the same. No positive immunostaining was found in the control cardiac tissues. Conclusion The results suggest that the early myocardial ischemia may have revealed as the same changes as myocardial infarction at molecular level, indicating that NF-?B p65 may be used as a marker for postmortem diagnosis of the early acute myocardial ischemia by immunohistochemical technique.

OBJECTIVE To investigate the time of aerosol inhalation using Budesonide Suspension and Mucosolvan drived by oxygen on prevention of pharyngeal and oral mucosa damage induced by radiotherapy in nasopharyngeal carcinoma (NPC). METHODS All 108 cases with NPC were divided into 4 groups, each group 27 cases. In 3 treatment groups,aerosol inhalation using Budesonide Suspension and Mucosolvan drived by oxygen were began at the initial , after 5 times, after 10 times radiotherapy respectively. In control group, aerosol inhalation was not used. The rates of third degree of pharyngeal and oral mucosa injury during different dose of radiotherapy were compared among the 4 groups. RESULTS At the dose below 30 Gy, there were no case of third degree of pharyngeal and oral mucosa injury occurred in 3 treatment groups, one case in control group. At the dose of 30 to 40 Gy, third degree injury occurred in all groups, there was no statistical difference among the 4 groups. At the dose over 40 Gy, the patients of third degree injury in group 1 and 2 were less than that in control group. There was no difference between group 3 and control group. CONCLUSION The right time of aerosol inhalation using Budesonide Suspension and Mucosolvan drived by oxygen for prevention of pharyngeal and oral mucosa complication induced by radiotherapy in NPC is at 5 to 10 times after radiotherapy.

Objective To investigate the clinical efficacy and safety of two chemotherapy regimens based on oxaliplatin combined with TS-1 or capecitabine in the treatment of advanced colon cancer.Methods 53 advanced colon cancer patients were involved in this study.26 patients were given the regimen of SOX:oxaliplatin (130 mg/m2) was administered intravenously in three hours on day 1,while S-1 (40 mg/m2) was administered orally twice daily for 14 consecutive days.27 patients were given the regimen of XELOX:oxaliplatin capecitabine was administered intravenously in three hours on day 1,while capecitabine (1000 mg/m2)was administered orally twice daily for 14 consecutive days.The regimens were repeated every 3 weeks.The efficacy and toxicy were evaluated after 2 cycles teatment.Results All the 53 patients were eligible for enrollment and the patients were appreciable for efficacy and toxicy.The objective response rates (RR) were 46.1％ (12/26) and 48.1％ (13/27) in the SOX group and XELOX group respectively.The disease control rates (DCR) were 76.9 ％ (20/26) and 74.1％ (20/27).There was no significant difference in RR.The objective side effects associated with the two regimens were bone marrow suppressive,digestive system reaction (diarrhea or vomiting),peripheral neuropathy and hand-foot syndrome on mostly graded 1 or 2.Compared to the XELOX group,nausea vomiting of the SOX group were higher (x2 =4.462,P =0.035),but diarrhea and hand-foot syndrome of XELOX group was obviously higher than that of SOX group [44.4 ％ (12/27) vs.19.2 ％ (5/26) (x2 =4.366,P =0.001),44.4 ％ (12/27) vs.3.8 ％ (1/26) (x2 =11.699,P=0.037)].Conclusion There are no significant difference in the efficacy of the two chemotherapy regimens and the side toxicities are all well tolerable.

Objective: To investigate the expression of EMS1 in laryngeal carcinoma and its clinical signifi-cance. Method:The expression of EMS1 protein was measured in 40 samples of, 40 samples of para carcinoma tis-sues (which were near to cutting margin of laryngeal carcinoma tissuse over 0. 5 cm) ,and 20 samples of normal la-ryngeal mucosa as controls by Flow Cytometere( Epics-XL Ⅱ ). Results:The quantity and percentage of EMS1 pro-tein expression in laryngeal carcinoma tissues was significantly higher than those in para carcinoma and in normal laryngeal mucosa tissues respectively(P<0. 05). There was no significant expression difference between the para carcinoma tissues and normal laryngeal mucosa tissues. There were positive correlation between the expressions of EMS1 protein and metastasis, pathological grade and clinical stage in laryngeal carcinoma. But there were not rela-tionship with patients' clinical classification, tumor size, smoking history, age and sex. Conclusion: The high ex-pression of EMS1 may contribute to the carcinogenesis and development of laryngeal carcinoma. The expression of EMS1 protein is an important index of judging differentiation, infiltration, metastasis and staging of laryngeal car-cinoma.

Objective To investigate the effect of curcumin on the expression of heat shock protein 70 (HSP70) in the hippocampus and spatial learning ability in APPswe/PS1△9 transgenic mice,and provide a new theoretical basis for the treatment of Alzheimer' s disease (AD).Methods Mice were divided into wild-type (WT) group,APP/PS1 transgenic group,curcumin-treated group,quercetin combined with curcumin-treated group.The 5-month-old mice in curcumin-treated group received 150 mg · kg-1 · d-1 curcumin by intraperitoneal injection for four consecutive weeks.In addition to curcumin,the mice in quercetin combined with curcumin-treated group also received quercetin 50 mg· kg-1 · d-1 intraperitoneally for four consecutive weeks.The expression of HSP70 in the hippocampus of APP/PS1 mice was detected by Western blot.Learning and memory ability of APP/ PS1 mice were measured by Morris water maze test.The latency of finding the platforms and the number of crossing the platforms were observed.Results The results of Western blot analyses showed that the expression of HSP70 in the hippocampus of APP/PS1 transgenic mice decreased significantly compared with that of WT mice (OD:APPswe/PS1△9:0.31±0.14;WT:1.14±0.51;P＜0.01).The expression of HSP70 in curcumin-treated group significantly increased compared with that of APP/PS1 transgenic group (OD:0.91±0.20 in curcumin-treated group;P＜0.05).The results of water maze test showed that compared with WT group,in APP/PS1 group,the latency of finding the platforms was significantly longer (APPswe/PS 1 △9:(82.3 ± 6.8) s;WT:(19.5±4.4) s),and the number of crossing tbe platforms were significantly decreased (APPswe/PS1 △9:(2.7± 1.1);WT:(5.2± 2.1)).In curcumin-treated group,the latency of finding the platform ((47.7±7.6) s) was significantly shorter and the number of crossing platform ((4.5±1.9) s) was more than those in APP/ PS1 group.However,in quercetin combined with curcumin-treated group,the latency of finding the platform was significantly longer ((69.7±9.1) s) and the number of crossing platform (3.2±1.6) was decreased compared with those in curcumin-treated group.Conclusion Curcumin can promote the expression of HSP70 in the hippocampus of APP/PS1 transgenic mice,thus improve spatial learning ability of APP/PS1 transgenic mice.These results provide evidences that curcumin may have potential therapeutic value for the treatment of AD.

0.05). Conclusion Both acanthopanasia and hoelen have no mutagenic toxicity founded on the genetic damage of somatic cells,and have significant antagonistic effects on the genetic damage induced by cadmium sulfate,and they are good antimutagents.