Objective To investigate the effect of thymopentin combined with routine medication on serum interleukin 17A(IL-17A),chemokine 12 (CXCL12) levels and transient receptor potential channel 1 (TRPC1)expression in elderly patients with chronic obstructive pulmonary disease (COPD).Methods 156 COPD patients were selected as study objects.They were randomly divided into two groups(n =78) according to the digital table.The control group received routine treatment,and the observation group received subcutaneous injection of thymopentin on the basis of routine treatment.Before and after treatment,the CD3+,CD4+,CD8+,CD4+/CD8+ levels were tested to compare the immunologic function of the two groups.Before and after treatment,the FEV1,FEV1/FVC,PEF were tested to compare the pulmonary ventilation function of the two groups.The IL-17A,CXCL12 levels in peripheral blood and TRPC1 level in bronchoalveolar liquid before and after treatment were tested and compared between the two groups.The adverse effect was compared.Results Before treatment,the CD3+,CD4+,CD8+ and CD4+/CD8+ between the two groups had not statistically significant differences (all P ＞ 0.05),which of the control group after treatment had no significant change (all P ＞ 0.05),the CD3+,CD4+,CD4+/CD8+ of the observation group [(65.17 ± 2.39) ％,(41.06 ±2.15) ％,(1.50 ± 0.74) ％] were significantly higher than control group [(52.66 ± 2.38) ％,(32.30 ± 2.05) ％,(0.80 ± 0.81) ％] (all P ＜ 0.05),and the CDs+ of the observation group [(24.02 ± 2.23) ％] was significantly lower than control group[(32.66 ± 1.97) ％,P ＜0.05].Before treatment,the FEV1,FEV1/FVC,PEF,the IL-17A,CXCL12 levels and TRPC1 level had no statistically significant differences between the two groups(P ＞ 0.05).After treatment,the peripheral blood IL-17A [(24.18 ± 3.69) pg/mL],CXCL12 levels [(193.50 ± 2.90) pg/mL] and TRPC1 level in BALF [(7.69 ± 1.14)ng/L] in the observation group decreased significantly(P ＜ 0.05),which in the control group also decreased significantly [IL-17A (34.25 ± 3.74) pg/mL,CXCL12 (205.37 ± 3.21) pg/mL,TRPC1 (14.25 ± 1.20)ng/L] (P ＜ 0.05),which of the observation group were significantly lower than those of the control group(all P ＜0.05).The incidence rates of adverse effects of the two groups were t6.67％,12.82％,there was no statistically significant difference(P ＞ 0.05).Conclusion Thymopentin combined with conventional therapy can effectively improve the elderly COPD patients'immunologic function and pulmonary ventilation function,decrease the inflammatory factor,relieve inflammatory reaction and it is safe and effective.

Pediatric acute myeloid leukemia(AML)is still a fatal disease.Recent evidence based clinical study indicated intensified chemotherapy contribute to improvement of pediatric AML survival,while supporting care was an important measurement to let patients tolerate more and more intensifying chemotherapy.Recent series of clinical trial reveals that benefit from more intensified chemotherapy will be limited.Combined risk stratification of genetics and minimal residual desease monitoring may further increase the survival.Tyrosine kinase inhibitors,Gemtuzumab ozogami-cin and Chimeric antigen receptors modified T cell based targeted therapy could be the only way to cure resistant AML. In this review,advance in conventional chemotherapy,hematopoietic stem cell transplantation,and targeted therapy for pediatric AML were reviewed.

BACKGROUND:Anterior dislocation of the shoulder joint is mainly due to trauma caused by anterior labral capsular ligaments complex functional deficiency. At present, the main clinical use is conservative treatment, but the long-term effect is poor, and cannot achieve the desired results.
OBJECTIVE:To study the biomechanical characteristics of dual fixed anchors and their effects in recurrent anterior dislocation of the shoulder with severe bone defect.
METHODS:According to the treatment, 88 patients with recurrent anterior dislocation of the shoulder with severe bone defect were divided into conservative treatment group (n=44) and dual fixed anchor group (n=44). Japan AG-ISOKN material tensile tester was used to measure biomechanical properties of dual fixed anchors. Conservative treatment group received closed manipulative reduction. Dual fixed anchor group received dual fixed anchor fixation. The repair effects were compared between the two groups.
RESULTS AND CONCLUSION:(1) Biomechanical parameters:in dual fixed anchor bending test, limit strength was (3 534±321) N;limit displacement was (21.12±2.50) mm;the yield load was (2 342±154) N;yield shift was (13.10±53) mm. Maximum axial pul out force was (809±25.2) N. The maximum spin torque was (1.2±0.3) N?m. (2) Clinical test results:Total effective rate was 96%in the dual fixed anchor group and 86%in the conservative treatment group (P<0.05). Ranges of motion of anteflexion and lift and shoulder joint stability score were significantly higher in the dual fixed anchor group than in the conservative treatment group during final fol ow-up (P<0.05). Range of external rotation motion was significantly smal er in the dual fixed anchor group than in the conservative treatment group (P<0.05). The complication rate was lower in the dual fixed anchor group (11%) than in the conservative treatment group (25%) (P<0.05). (3) Results verified that dual fixed anchors have good biomechanical characteristics, may have ideal repair effect in the treatment of recurrent anterior dislocation of the shoulder with severe bone defect, and can improve functions and activities of the shoulder joint. The low complication rate is more conducive to shoulder reconstruction.

Vibrio parahaemolyticus is an important food-borne pathogenic bacterium that widely exists in all kinds of seafood.As the traditional media and method were very costly-time and money,a new chromogenic medium(HKC vibrio)was designed in this assay.Through detecting the artificially contaminated samples and natural samples,the sensitivity,specificity and detection effect of HKC vibrio were studied and compared with CHROMagar vibrio and TCBS.The results showed that HKC vibrio had the same sensitivity as CHROMagar vibrio and TCBS,also had highly specificity.In conclusion,HKC vibrio was an invaluable medium which may improve the detection efficiency of Vibrio parahaemolyticus dramatictly.

Cochleae of guinea pig, cat, and human fetus were studied under SEM. After tbe treatment with osmic and tannic acids, they were dehydrated by a critical point drier, then coated with carbon and gold, and observed under a JSM-35 scanning electon microscope.The organ of Corti looks like a spiral staircase winding around the modiolus. It is wider at apex and narrower at base. The vestibular membrane is composed of squamous epithelia covered with microvilli. The tectorial membrane is mainly composed of parallel fibrils, but at its surface and marginal region, the fibillae interwoven. There are three rows of outer hair cells (OHC) and one row of inner hair cell (IHC) in the organ of Corti, and the head plate of pillar cells is set in between both kinds of cells. The stereocilia of OHC are arranged in W-form, whereas those of IHC are in a curved arrangement. All the cells observed have microvilli, however, in some of the animal specimens, the stereocilia are irregularly arranged, damaged or entirely disappeared owing to noise exposure.In the four-month fetal cochlea, the cuticular plates of OHC looks just like velvet balls, which afterwards become W-shaped stereocilia, while on the surface of IHC, primitive hair bundles develop into a curved arrangement.Certain broken organ of Corti shows cylindrical body of OHC and its cup-like supporting structure. Phalangeal processes of Deiter ceils and OHC are obliquely arranged forming an angle. The pillar cell body is thin above and thick below, and twined with very thin nerve network.The tunnel cavity can be exposed by removing the roof structures, then longitudinal nerve fibers going along the tunnel bottom may be clearly seen. These fibers belong to the spirotunnel bundle——an efferent branch of olivo-cochlear bundle.

Based on the analysis of 26 malignant trophoblastic tumor patients detected by CDFI (Color Dopper Flow Image) and PD between 2001 and 2005,the article is an attempt to explore the clinical significance of CDFI and PD in treating the trophoblastic diseases in the early stage,including diagnosing the pathologic process,judging the chemotherapy effect and predicting the pathologic changes.

Objective To investigate the clinical efficacy of porous calcium phosphate bioceramics (PTCP)for treating stage FicatⅢ non-traumatic osteonecrosis of the femoral head(ONFH)in the patients aged below 60 years old.Methods A total of 41 patients (63 hips)with non-traumatic ONFH in our hospital from March 2006 to July 2009 were collected and divided into two groups.The group A (n=1 6)was performed the core decompression only,while the group B (n=25)was performed the core de-compression plus through the head-neck junction of the femur to opening the window light bulb shape focus debridement and impac-ting bone grafting combined with PTCP implantation.The various clinical evaluation indicators were observed and evaluated.Results No statistically significant differences in PRI were found at each time point in the group A.PRI at postoperative 1 month in the group B was significantly decreased than before operation,hereafter,the stable status was maintained and slightly risen again until postoperative 3 years(P =0.033).The Harris scores at postoperative all time points in the group A were significantly lower than those in the group B.The necrosis areas at postoperative 24 months,3,4,5 years in the group B were significantly decreased com-pared with before operation and lower than those in the group A.Conclusion PTCP implantation has satisfactory effect for the treatment of stage FicatⅢ non-traumatic ONFH in the patients aged below 60 years old.

Objective To explore the application effect of self management mode during internship of nursing interns.Methods 100 nursing students were randomly divided into the experimental group (self management group)and the control group(traditional pattern group),50 cases in each gronp,The control group adopted strict practice management according to the traditional method,the experimental group adopted self management mode.Times of being late,absenteeism,academic achievement and operation performance were compared between two groups.Results Times of being late,absenteeism in the experimental group were less than those of the control group,while academic achievement and operation performance in the experimental group were significantly lower than the control group,the difference between the two groups were statistically significant.Conclusions The implementation of self management mode for nursing interns can effectively improve the nursing students' ability of self management,improving the teaching quality,ensure nursing safety.It is worthy of clinical application.

Objective To discuss the indications and efficacy for treating tibia insertion avulsion fracture of the posterior cruciate ligament(PCL)by the mean of the end of ligament suture via arthroscopy.Methods Twenty-one cases of tibia insertion avulsion fracture of PCL were treated with suture the end of ligament via arthroscopy from June 2007 to June 2009,including 14 males,7 females,with a mean age of 31.5 years(range,13-52 years).The insertion avulsion fragment was fixed to the tibia with two strands of 5Ethibond threads via arthroscopy.Two bone tunnels(2.5 mm in width)were made from the anterior part of the tibia to the posteromedial(at 4:30 toward)and posterolateral(at 7:30 toward)of the tibia bone bed respectively.Then the sutures were pulled out through the two bone tunnels and tied to the door-nail.Reduction and healing of the avulsion fragment,relaxation and mobility degree of knee,and the influence of osteoepiphysis to teenage patients were observed after surgery.KT-2000 value and Lysholm score were calculated.Results All cases were followed up from 10 to 24 months,with an average of 13.5 months.All the fragments were united without displacement in all cases according to X-ray films.One case showed posterior drawer test(+),but the tibia insertion was healed.The knee extension in all cases was unlimited,and 10° to 15° flexion limitation was found in 2 cases.The average range of flexion was 140.5°±3.8°.On average,KT-2000 value was(1.2±0.4)mm,Lysholm score was 95.2±2.7.Conclusion For treating the avulsion fracture of PCL,suture the end of ligament via arthroscopic transfixion can get a reliable fixation and a satisfied function of the knee,especially,which is suitable for the small avulsion fracture fragments,the adolescent patients with premature epiphysis.

Objective To investigate serum leptin and adiponectin levels and their relationship in elderly coronary artery disease (CAD).Methods A total of 88 subjects aged 55 yeas or older were enrolled in which coronary angiograms were performed.The subjects were divided into CAD group (52 patients) and control group (36 patients) by angiography.Serum concentrations of leptin and adiponectin were evaluated by ELISA system.Results Serum concentrations of leptin were significantly increased in CAD group compared with that in control group [(9.38 ±3.51) μg/L vs.(7.27 ±3.25) μg/L,P ＜0.05].On the contrary,serum concentrations of adiponectin were significantly decreased in CAD group compared with that in control group [(11.08 ±3.87) mg/L vs.(13.34 ±4.94) mg/L,P＜0.05].Logistic regression analysis revealed that only serum leptin was the independent risk factor for CAD.Serum adiponectin concentrations had a negative relationship with leptin concentrations (r =-0.320,P =0.002).Conclusion Lower serum adiponectin levels are related to higher serum leptin concentrations.Elevated serum leptin but not decreased serum adiponectin is the independent risk factor for CAD.