Objective: To test whether depressive college students had sociotropic self-schemata. Methods: A total of 500 college students were surveyed by CES-D and SAS, and 77 college students were chosen for further experiment: 24 with depressive mood, 12 with anxious mood, 17 with both depressive and anxious mood, and 24 normal ones. Sociotropic adjectives were strictly developed, and the 4 groups (depressive vs. anxious vs. depressive and anxious vs. normal) were given Self-referent Encoding Task. Results: ①The depressive group showed significantly less positive adjectives and more negative adjectives for self-descriptive than the normal group (P

Objective To test whether adolescent rats are more sensitive than adult rats to morphine' s rewarding actions and locomotor activating effects. Methods Conditioned place preference(CPP) procedure was used to examine drug-induced reward and locomotor activity. Adolescent ( Postnatal day 35 ) and adult ( P67 ) male rats received either saline or morphine (3 mg/kg) for 6 days and the locomotor response in the drug paired chamber was recorded during conditioned training. CPP effects were tested 24h after the last training. Results In adolescent rats, morphine CPP effect was not present [ saline group (20. 89 ±31. 14) s, morphine group (90. 75 ± 27.91)s, P = 0. 15], however, morphine CPP effect occurred in adult rats[saline group (31.5 ± 41. 24) s, morphine group(266. 13 ± 32. 26) s, P < 0. 001 ]. Adult rats presented higher CPP score than adolescent rats(P < 0.001). The locomotor response was significantly higher in day 3 ～ 6 than day 1 in the adolescent treated with morphine (P<0.05 or <0. 01). The locomotor response was significantly higher in day 2-6 than day 1 in the adolescent treated with morphine(P<0. 05 or < 0. 01 ) .which suggested that both adolescent and adult rats exhibited similar behavioral sensitization to morphine administration. Two-way ANOVA indicated that the main effect of age was not significant(F_(1,14) =0. 33, P = 0. 57) ,the interaction effect of age x treat was not significant(F_(5,70) = 0.85, P = 0.52) .there was no age difference between adolescent and adult rats in behavioral sensitization effects. Conclusion Compared with adult rats, adolescent rats don' t present morphine CPP, but show the similar morphine behavioral sensitization.

Objective To explore the mediating effects of college students＇ online social support on the relationship between online self-disclosure and internet altruistic behavior.Methods Using cluster random sampling,the self-disclosure questionnaire for Chinese undergraduates with their internet friend,the online social support scale of adolescence and the internet altruistic behavior scale of undergraduates were applied to 351 college students.Results The score of college students＇ online self-disclosure,online social support and internet altruistic behavior were respectively(2.22±0.45),(2.93±0.59) and (2.04±0.46).The college students＇ online self-disclosure,online social support and internet altruistic behavior were positively correlated with each other (P＜0.01).College students＇ online self-disclosure not only predicted internet altruistic behavior directly,but also predicted internet altruistic behavior indirectly through online social support.The direct effects and indirect effects were 0.200 and 0.265.The ratio of the mediating effect on the total effect was 57％.The fit index of mediation model was x2/df=2.87,RMSEA =0.07,CFI =0.94,TLI =0.93,GFI=0.92,IFI=0.94,NFI=0.91.Conclusion The college students＇ online self-disclosure not only affects the interuet altruistic behavior directly,but also can take the same effect on online social support indirectly.

Objective To establish a method of fingerprint analysis on Spica Prunellae by HPLC.Methods The HPLC method was used,chromatographic conditions were Betasil C18 column(250 mm ? 4.6 mm,5 ?m),acetonitrile-0.1% phosphric acid system as mobile phase,0-50 min acetonitrile 10%→25%,50-60 min acetonitrile 25%→32%;flow rate 0.7 mL/min;detective wavelength at 210 nm,column temperature 25 ℃.Results Establishing the fingerprint of Spica Prunellae,16 common peaks were found in the HPLC fingerprint of Spica Prunellae and good similarities with correlation coefficients over 0.95 were found in the fingerprints between the herbs from different sources and standard fingerprint,which could be utilized for the identification of Spica Prunellae.Conclusion The method is accuate,simple,and could be used for the quality control of Spica Prunellae.

Objective To study the relationship between serum high sensitive C-reactive protein(Im-CRP) and acute coronary syndrome(ACS). Methods We detected and analyzed the levels of hs-CRP in 38 clinical deft-trite patients with ACS. Results Patients with ACS had significantly higher serum hs-CRP levels than the control group(P < 0.05). Conclusion Serum hs-CRP level has high correlation with ACS, and the hs-CRP maybe a arthero-sclerosis marker,which reflected the process of coronary artery disease.

In this paper, patients with the left ear hearing loss, aural fullness. CT: the left wing palatine nest sites. MRI: on the left wing palatine timid benign lesions; Electric listening: conductive deafness. Acoustic immittance: B type curve. Clinical diagnosis: the left wing palatine fossa cyst, left ear secretory otitis media.
Acoustic Impedance Tests ; Auditory Perception ; Cysts ; diagnosis ; Deafness ; diagnosis ; etiology ; Hearing Loss, Conductive ; etiology ; Humans ; Magnetic Resonance Imaging ; Otitis Media with Effusion ; diagnosis

OBJECTIVE: Assessed the feasibility of application of free fibular flap and clinical significance of pre-operational contrast enhanced CT angiography in functional reconstruction of oral and maxillofacial hard and soft tissue defects. METHOD: Eight cases with mandibular and soft tissue defects received a free fibula flap using arteriovenous anastomosis anastomosis method. The relationship between fibula flap design, size, repair parts and survival were analyzed. Preoperative enhanced CT angiography (CTA) examination was conducted to detect any abnormal blood vessels in fibula flap valve area. RESULT: Peroneal artery and posterior tibial artery variation was identified in one case of gums cancer, who used other muscle flap. Free fibula flap in the other 7 cases survived, which carried a skin island with an area ranging from 6 cm x 2 cm to 10.0 cm x 3.5 cm. Postoperative facial appearance, functionality, dental occlusion and voice function was normal. Lower limb function returned to normal from 2 weeks to 4 months after surgery. CONCLUSION CTA examination of the free vascularized fibula flap in the preoperative evaluation of the donor site is a valuable tool. Free fibula flap of bone, periosteum and bone marrow has a dual blood supply and are highly resistant to infection after transplantation. Personalized shaping osteotomy allowed for accurate recovery of mandibular alveolar patterns. Furthermore, the height and width of the fibula and the thickness of cortical bone is suitable for dental implants. Free fibula flap skin island can also be used to monitor the post-operative blood supply and is an ideal technique for mandible and soft tissue defects reconstruction as well as functional restoration.
Adult ; Aged ; Angiography ; methods ; Female ; Free Tissue Flaps ; blood supply ; Humans ; Male ; Maxillofacial Injuries ; surgery ; Middle Aged ; Mouth Neoplasms ; surgery ; Reconstructive Surgical Procedures ; methods

Objective To investigate the effects of cold preservation on the expression of GATA in intrahepatic bile duct.Methods The intrahepatic bile duct tissues of SD rats were obtained by collagenase perfusion combined with mechanical separation.After being cut into fragments,the intrahepatic bile duct tissues were cultured in rat tail collagen gel for 48 hours before experiment.All the rats were divided into the control group,cold preservation 1 hour (CP1 h) group and cold preservation 12 hours (CP12 h) group.There were 5 rats in each group.The mRNA and protein expressions of GATA were detected by Real-Time polymerase chain reaction and Western blot.Measurement data with normal distribution were presented as (x) ± s.Comparison among 3 groups was done by ANOVA and pairwise comparisons were done by LSD test.Results The mRNA expressions of GATA3,GATA4,GATA6 were detected,while the mRNA expressions of GATA1,GATA2 and GATA5 were undetectable in intrahepatic bile duct tissue of the control group.The mRNA expressions of GATA4 in the CP1 h group,CP12 h group and the control group were 0.72 ± 0.08,0.56 ± 0.07 and 0.96 ± 0.06,with significant difference among the 3 groups (F =38.981,P ＜0.05).The mRNA expression of GATA4 in the CP12 h group was significantly lower than that in the CP1 h group and the control group,and the mRNA expression of GATA4 in the CP1 h group was significantly lower than that in the control group (P ＜ 0.05).The mRNA expression of GATA6 in the CP1 h group,CP12 h group and the control group were 0.83 ± 0.07,0.68 ± 0.12 and 0.98 ± 0.12,with significant difference among the 3 groups (F =10.175,P ＜ 0.05).The mRNA expression of GATA6 in the CP12 group was significantly lower than that in the CP1 h group and the control group,and the mRNA expression of GATA6 in the CP1 h group was significantly lower than that in the control group (P ＜ 0.05).The mRNA expressions of GATA3 in the CP1 h group,CP12 h group and the control group were 0.92 ± 0.06,0.89 ± 0.05 and 0.98 ± 0.11,with no significant difference among the 3 groups (F =1.674,P ＞ 0.05).The protein expressions of GATA4 in the CP1 h group,CP12 h group and the control group were 0.78 ± 0.07,0.64 ± 0.06 and 0.99 ± 0.10,with significant difference among the 3 groups (F =24.211,P ＜ 0.05).The protein expression of GATA4 in the CP12 h group was significantly lower than that in the CP1 h group and the control group,and the protein expression of GATA4 in the CP1 h group was significantly lower than that in the control group (P ＜ 0.05).The protein expressions of GATA6 in the CP1 h group,CP12 h group and the control group were 0.90 ± 0.04,0.75 ±0.06 and 0.98 ±0.11,with significant difference among the 3 groups (F=11.651,P＜0.05).The protein expression of GATA6 in the CP12 h group was significantly lower than that in the CP1 h group and the control group (P ＜ 0.05).Conclusion The expressions of GATA4 and GATA6 in the intrahepatic bile duct tissues are decreased significantly after cold preservation,which indicate that GATA4 and GATA6 might be involved in the pathophysiological process of the bile duct after cold preservation.

Objective To investigate the genetic polymorphism of DXS8378 STR locus of chromosome X in Chinese Lisu,Pumi and De'ang populations in Yunnan and construct relative standard allelic ladders.Methods After being amplified by PCR,different STR allelic fragments were isolated from the PAG electrophoresis.The STR allelic fragments were extracted by kit and reamplified by PCR to obtain purified allelic fragments.Next,the purified allelic fragments were subcloned individually into the PUC plasmid vectors,and the size and structure of the inserts were confirmed by the analysis of their DNA sequences.Then we transfected it into competent E.coli DH5?TM cells,and finally,the recombinant plasmids DNA with the inserts were used as template for reamplification to generate the standard ladders.Results The standard allelic ladder for DXS8378 locus was obtained,with which the genetic polymorphisms of DXS8378 locus in three Chinese populations in Yunnan were studied.Conclusion The standard ladder made by this method is excellent,and DXS8378 is powerful for forensic practice in Chinese population.

According to their polypeptide length and number of d is ulphide bonds, disintegrins are divided into five groups, including short-sized disintegrins, medium-sized disintegrins, long-sized disintegrins, dimeric dis integrins and disintegrin-like molecules released from the disintegrin-like do mains of P-III snake venom metalloproteinases. The integrin-inhibitory activit y of disintegrins, to inhibit platelet aggregating, cell attaching and angiogene sis, depends on the active tripeptide RGD, the appropriate pairing of cysteine r esidues, the amino acids adjacent to the RGD motif within the integrin-binding loop and the C-terminus of the disintegrin polypeptidesl as well.