1.An Integrated Strategy for Functional Analysis in Large-scale Proteomic Research by Gene Ontology
Dong LI ; Jianqi LI ; Shuguang OUYANG ; Songfeng WU ; Jian WANG ; Xiaojie XU ; Yunping ZHU ; Fuchu HE
Progress in Biochemistry and Biophysics 2005;32(11):1026-1029
Data analysis poses a significant challenge to the large-scale proteomics studies. Based on the structured and controlled vocabularies-Gene Ontology (GO), and the GO annotation from related databases, a strategy composed of several programs and local databases is developed to identify the functional distribution and the significantly enriched functional categories of the proteomic expression profile. It would be helpful for understanding the overall functions of these identified proteins and supply the fundamental information for further bioinformatics exploration. This strategy has been successfully used in the Human Fetal Liver (HFL) proteomic research, which is available online at http://www.hupo.org.cn/GOfact/.
2.Construction and Application of a Large-scale DNA Sequence Analysis System Based on PC/Linux
Chenggang ZHANG ; Shuguang OUYANG ; Shaowe ZHANG ; Xianghu QU ; Yongtao YU ; Gangqiao ZHOU ; Songfeng WU ; Fuchu HE
Progress in Biochemistry and Biophysics 2001;28(2):263-266
More and more DNA sequences have been obtained since the start-up of human genome project. Powerful system is badly needed for data mining on these DNA sequences. Based on a personal computer and Linux operating system, the Phred/Phrap/Consed software and Blast software were used to construct a platform for batch analysis of the sequences, including identifying raw DNA sequence from chromatogram file, vector sequence removing, contig analysis (sequence assembly), repeat sequence identifying and sequence similarity analysis. Result demonstrated that this robust platform could accelerate data analysis for large-scale DNA sequencing.
3.Clinical application of intraoperative intraperitoneal hyperthermic chemotherapy using sustained-release .fluorouracil in radical gastrectomy for gastric cancer
Nengbin WAN ; Li ZHANG ; Chaohui ZUO ; Xiao HE ; Jingguan LIN ; Shuguang PAN ; Bin YIN ; Wei LUO ; Haizhen ZHU ; Yongzhong OUYANG
Journal of Chinese Physician 2012;14(6):763-766
ObjectiveTo investigate clinical application of intraoperative intraperitoneal hyperthermic chemotherapy using sustained-release fluorouracil in radical gastrectomy for advanced gastric cancer.MethodsThe clinical data of 280 advanced gastric cancer patients admitted from September,2002 to September,2010 were analyzed retrospectively.They were divided into three groups randomly and followed up.The postoperative morbidity,the mortality and the overall survival rates were evaluated.ResultsThere were no significant differences in these three groups with respect to postoperative morbidity ( P > 0.05 ).The incidence of recurrence in intraperitoneal chemotherapy using sustained-release fluorouracil ( treatment group) was significantly lower than those of intraperitoneal chemotherapy and operative treatment( 16.18%,37.61% and 41.28%,P <0.05).The 1,3- and 5-year overall survival rates of treatment group were 85.51%,61.28% and 53.67%,respectively,and the 1-,3- and 5-year overall survival rates were 84.11%,39.98% and 28.12%,and 81.28%,29.88% and 25.21% respectively in intrapeitoneal chemotherapy group and operative group.1-year overall survival rate had no significant differences among three groups with respect to ( P>0.05).3-and 5-year overall survival rates in treatment group were higher signfficantly than those of intraperitoneal chemotherapy and operative treatment( P<0.05).Conclusions Intraoperative intrapeitoneal hyperthermic chemotherapy using sustained-release fluorouracil is a kind of convenient,safe,and highly effective comprehensive treatment method,and it can kill isolated intraperitoneal cancer cells.It may reduce postoperative recurrence and improve survival rates.