Objective To investigate the apoptosis of human bile duct carcinoma cell induced by Apoptin gene transfection in vitro. Methods Apoptin gene was amplified by polymerase chain reaction (PCR) and then inserted into pAdtrack-CMV containing green fluorescent protein gene through T4 DNA ligase. The reconstructed plasmids were identified by enzyme digestion and sequencing. Apoptin expression vector was transfected into human bile duct carcinoma cell line QBC_(939). The apoptosis of human bile duct carcinoma cell induced by Apoptin gene was detected by TUNEL. Results Reconstructed pAdtrack-CMV containing Apoptin gene was obtained successfully. TUNEL test showed that there was significant difference in apoptosis index between Apoptin gene transfection and the control group (P

We introduced Case based learning into traditional bilingual teaching, dividing the hepatobiliary surgery teaching into four phases: preview, introduction, group discussion and estimation after class . Through the classroom organization analysis of common cases of liver and gallbladder surgery, we took students as the main body, teacher as the guide, to complete the bilingual teaching. Auxiliary case based learning used in bilingual teaching of liver and gallbladder surgery practice is beneficial to improving the quality of bilingual teaching, and helps to cultivate the students' clinical thinking. However, there are still some deficiencies of the case aided bilingual teaching, which needs continuous improvement.

Objective To understand the dietary intake of pregnant women, and try to give a reasonable sug-gestion to promote fetal development. Methods 400 pregnant women in Fushan city were enrolled in this study and their dietary intake were investigated, then the software of Analysis of pregnancy nutrition guidance system was used to make nutrition analysis. Results The ratio of the energy in each pregnancy provided by dietary protein, carbohy-drate and fat were relatively appropriate. But the intake of dietary calcium, ferrum, zinc, vitamin B1 and vitamin B2 in early pregnancy were very low, the intake were respectively 764.51 mg, 26.94 mg, 15.71 mg, 1.12 g, 1.13 g. Each pregnancy the ratio of high-quallty protein in total protein intake were more than 50%, an average protein RNI% was 48.50%. Conclusion The dietary style of pregnant women is relatively reasonable. But they should strengthened nutritional guide to intake more calciuro,ferrum, zinc,vitamin B1 and vitamin B2.

Objective To investigate the therapeutic measures of unresectable primary liver cancer (PLC) with transcatheter arterial chemoembolization(TACE).Methods The clinical data,treatment measures and followed up data of 312 patients with unresectable primary liver cancer from January 1991 to March 2010 were analyzed retrospectively.Of these 312 patients, different dosages, measures of transcatheter arterial chemoembolization together with immunotherapy and nutritional support treatments were given to the patients according to their own statements, including the patency of portal vein (evaluated by portography), the range, blood supply and lipiodol concentration of the lesions (evaluated by hepatic arteriography), and the systemic conditions.Some of these patients also accepted cryotherapy, radio frequency ablation (RFA), percutaneous ethanol injection therapy.Results Two hundred and eighty-seven patients were followed up (92％).Forty-two cases accepted the two-stage operation as the lesions were localized or reduced.In these 42 patients, 34 cases accepted the hepatectomy, 8 cases accepted the cryotherapy.The 1,3 and 5 year survival rate of all patients were 87.6％, 33.1％ and 13.2％.Conclusion TACE for unresectable primary liver cancer should be used individually and comprehensivly.Surgical treatment should be taken once the lesions can be resected.

Objective To study the therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene.Methods Subcutaneous human bile duct cancer was established in nude mice.Variations of tumor volume and histomorphology,side effects were observed after intratumoral injection of adenovirus containing Apoptin gene.Finally the mice were sacrificed for calculating the ratio of antitumor.Results Twelve days after treatment,the mean volume of the xenograft human bile duct cancer in the group of intratumoral injection of adenovirus containing Apoptin gene was(92.31?28.31)mm,which was reduced significantly compared with that of adenovirus infection without apoptin gene(288.86?113.13)mm and control group(344.86?113.87)mm.The ratio of antitumor was 72.10%,which was significantly higher than that in control group(11.9%).During the whole experimental course,no side effect was observed.The histological results demonstrated that the reduction of tumor growth was the result of apoptosis in bile duct cells,which was reduced by transfection of Apoptin gene.Conclusion The adenovirus vectors containing Apoptin gene may constitute a safe tool for the treatment of cholangiocarcinoma.

Objective To obtain magnetic resonance imaging(MRI) symptoms that could be used in medical diagnosis and identification by analyzing the diverse MRI appearance of brain multiple sclerosis(MS).Methods MRI findings of 41 samples of clinically definite MS, including the numbers, distributions, sizes, and shapes of the lesions were analyzed. The symbolic characteristics and enhanced expression are also covered.Results Brain MS might happen singly and multiply, and more frequently with multiple happening. In a multiple case, 4—15 lesions were observed the most. Some samples showed with diffuse lesions which were unable to be counted accurately presenting as a “dirty-appearing white matter”. The distribution of the lesions was observed most frequently at the two sides of periventricle, and following by the subcortical, corpus callosum, and brainstem. Only two lesions were observed at cerebrum. The size of most lesions range from a few millimeters to 2 cm, accounting for about 75% of the total samples. Lesions with size above 2 cm were seen seldomly, with the biggest one of 6—7 cm. According to the shapes and symptoms, lesions might be categorized as an acute and chronic ones. The acute lesions had a shape of oval or circle, with a swelling appearance, low signal of T_1WI, and isointensity or a slightly higher signal on the circle. T_2WI showed a high signal, with different increase and showing “core+lunar” sign. This kind of lesions showed an enhancement with the circle enhancement as the most typical one, which had a complete circle or non-conplete arc shape enhancement, even the big lesions. The chronic lesion might also be divided into two categories. One was the quasi-symmetric lesion, with the distribution along two sides of periventricle and with the shape of small puncture/patch, part of them merged into the big patch; another kind of lesions was found scattered sparsely at frontal, parietal lobes and two sides of the periventricle, with small patches in shape. The chronic lesions had an appearance of shrinking, sharp edge, even signals, and had no lunar circle phenomena and obvious enhancement.Conclusion Brain MS have diverse MRI appearence, with some of which are unique and symbolic.

Studies using isolated rat fundus strip preparation demonstrated that l-stephanine ( l-STP ) , l-roemerine ( 1-REM ), l-stepholidine (l-SPD), 1-tetrahydropalmatine ( l-THP ) and dl-tetrahydrode-berine ( THB ) possessed 5-HT blockade properties. 1-STP ( 16, 25 ?M ) produced competitve antagonism against 5-HT receptor on rat fundus, while higher concentration ( 50 ?M ) suggested a non-competi tive inhibition, with pA2' and2' values equal to 5.8 and 4.2 respectively. 1-REM was found to be a non-competitive antagonIst ( pD' 2 = 4.5). 1-SPD, 1-THP and THB cuased a parallel shift to the right of 5-HT concentration response curve without any significant changes in their maximum response, indicating that they were competitive antagonists of 5-HT receptor. The antagonistic potencies of 1-SPD, l-THP and THB were expressed as pA2 values: 6.2,5.2 and 5.0.

This study was designed to investigate modulation of phosphorothioate oligodexynucleotides of laminin receptor on MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Treated with a concentration of 12?mol/L LNR AS OD in cuture medium, the relative MMP 2 and MMP 9 mRNA level at 72h in human bile duct carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction. The results showed that the expression of MMP 2 and MMP 9 dropped obviously by AS OD group as compared with control group. The relative expression level of MMP 2 and MMP 9 gene mRNA decreased about 33 2% and 23 9% respectively. It is suggested that LNR AS OD is the regulator of MMP 2 and MMP 9 gene expression. It can decrease MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Understading the regulation of MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells might contribute to the development of a new preventive and theraputic strategy for tumor invasiveness.

To investigate the expression of laminin receptor in different bile duct carcinoma cell strains, a peritoneum infiltration screening model was established to screen out the different metastatic cell strains of bile duct carcinoma. Invasive experiments in vitro were adopted to validate the results. The difference of LNR expression was compared both in the high and low cell strains by flow cytometry analysis. The results showed that by using the peritoneum infiltration screening model, high and low metastatic QBC939 cell strains were successfully screened out. Invasive experiments in vitro also proved that the invasive ability of high cell strain was stronger than that of the low one. The positive rate of LNR in high strains (36 36?9 22)%was higher than that in the low ones(13 17?3 43)%, and the difference was significant( P

Objective To study the factors influencing outcome of acute arterial embolism in extremity.Method From Aug 1997 to Nov 2001, we have accomplished treatment in 41 cases of acute arterial embolism in extremity with Fogarty catheter.Result Among 41 cases,26 cases were succeeded in leg salvage,8 cases were subject to amputation and 7 cases were dead finally.Conclusions Durationand degree of ischemia in extremity,embolectomy completeness in the case of introoperation,reperfusive injury and myonephropathic-metabolic syndrome,Primary diseases and general conditions are main influencing factors in treatment of acute arterial embolism.