Objective　In order to understand situation and need s for health education of AIDS prevention in high school students in Anhui. Methods　1 617 students from 12 schools in 4 prefectures were sur veyed with questionnaires. Results　More than 50% students got the i nformation from newspaper, broadcasting, TV. Only 7.5% and 4.7% st udents acquired the info rmation from the subject lectures at school level and class level respectively. 14.4% students attended health education activities f or AIDS prevention. 10.9% students received relevant pro paganda materials, 77.8% students had never seen condom, 44.1% students knew the function of condom, and 8.6% students approved of premarital sexual life, of wh ich , ratio of male students were significantly higher than that of female students. More than 70% students were willing to care and help patients with HIV infectio n. Conc lusions　It is necessary and possible to develop health education for AI DS prevention in high school students. It is an important measure to develop hea lth education to block HIV transmission.

Objective To study the effect of ulinastatin on serum C-reactive protein (CRP) and exhaled nitric oxide(FeNO) in patients with bronchial asthma.Methods 98 patients with bronchial asthma were randomly divided into the control group (n =49 cases) and the observation group (n =49 cases).The patients in the control group were treated through the conventional treatment,while the patients in the observation group were treated through the conventional treatment plus ulinastatin.Pulmonary function,asthma symptom scores,serum CRP and FeNO were measured before and after treatment.Results FEV1％ and predicted PEF values after treatment were significantly increased (t =4.720,8.112,3.724,6.723,all P ＜ 0.05).Compared with the control group,the observation group after treatment increased more significantly (t =3.102,4.002,all P ＜ 0.05).After the treatment,the asthma symptom scores were significantly decreased (t =2.190,6.021,all P ＜ 0.05).Compared with the control group,the asthma symptom scores of the observation group significantly decreased (t =2.620,P ＜ 0.05).After treatment,FeNO and CRP were significantly decreased (t =9.124,13.076,5.772,7.064,all P ＜ 0.01).Compared with the control group,FeNO and CRP of the observation group significantly decreased (t =3.060,5.401,all P ＜ 0.05).Conclusion Ulinastatin can significantly decrease serum CRP and FeNO in patients with bronchial asthma.

Objective To investigate clinical manifestation and pathological changes and the expressions of Emerin protein and STA gene of Emery-Dreifuss muscular dystrophy (EDMD).Methods The clinical features and STA gene detection from one patient with EDMD were analyzed retrospectively. Results The onset age of this patient was in early childhood. The four limbs were progressive muscle weakness and muscular atrophy. There were joint contractures and cardiac involvement in the early stage. The serum muscle enzymes increased slightly. The pathological changes in muscles showed that the sizes of muscle fibers were different, the fibers became spherical and some fibers were replaced by fat. Because of normal spinal anterior horn cells and sural nerves, neurogenic muscular atrophy might be ruled out. Emerin protein could not be tested in striated muscle and cardiac muscle. No mutation of STA gene was found in this case. Conclusions Emery-Dreifuss muscular dystrophy is one particular type of muscular dystrophy. It is characterized with joint contractures and cardiac involvement in the early stage. Emerin protein is deficient in EDMD. There is no mutation of STA gene in sporadic EDMD.

Objective To study the effect of carvedilol on heart rate variability and plasma N-terminal brain natriuretic peptide(NT-proBNP) in patients with chronic heart failure.Methods 106 patients with chronic heart fail-ure were randomly divided into the two groups ..53 patients in the control group were treated by the conventional ther-apy plus metoprolol ,while 53 patients in the observation group were treated by the conventional therapy plus carve -dilol.They were treated for six months .Heart rate variability and plasma NT-proBNP were measured before and after treatment in the two groups .Results The total effective rate of the control group was 83.0%,which was significantly lower than 94.3% of the observation group (χ2 =6.26,P<0.05).Before treatment,SDNN,SDANN,RMSSD and PNN50 between the two groups had no significant differences (t=0.284,0.360,0.410,0.302,all P>0.05).After treatment,SDNN,SDANN,RMSSD and PNN50 were significantly increased (t=3.095,9.184,3.622,4.302,2.261, 4.522,2.921,2.992,P <0.05 or P <0.01).Compared with the control group,those in the observation group increased more significantly (t=8.065,3.116,3.209,2.171,P<0.05 or P<0.01).After treatment,plasma NT-proBNP were significantly decreased in the two groups (t=7.093,9.773,all P<0.01).Compared with the control group,plasma NT-proBNP of the observation group decreased more significantly (t=4.773,P<0.01).Conclusion Carvedilol can more significantly improve heart rate variability and plasma NT-proBNP in patients with chronic heart failure.

Objective To study the causes and clinical features associated with hoarseness in children.Meth-ods The data of 4 668 children suffering from hoarseness were analyzed with electronic-nasopharyngolaryngosco-py retrospectively.ResuIts The top 6 common causes of hoarseness were chronic laryngitis (1 817 cases,38.9%), vocal cord nodules (1 494 cases,32.0%),vocal hypertrophy (560 cases,12.0%),the paralysis of vocal cord (373 cases,8.0%),congenital sulcus vocalis (149 cases,3.2%)and recurrent respiratory papilloma disease (140 cases,3. 0%).These six diseases were common in children with hoarseness with 4 533 out of 4668 (97.1%);of the chil-dren were 2 to 10 years old as the largest age group (65.8%,3 072/4 668)and the ratio of male and female was 2. 48:1.The top six diseases were statistically distributed in different age groups (P<0.01).ConcIusion The main causes of hoarseness were different in different age groups.The children aged 2 to 10 years old made up the largest group (3 072/4 668),showing the greatest incidence of hoarseness in children .The incidence for male patients was higher than female and the chronic laryngitis was the top disease.Hoarseness in children was different from that of adults so that to understand the characteristics of this disorder in children will be beneficial to the proper diagnosis and effective treatment.

Objective To study the impact of alprostadil on serum high-sensitivity C-reactive protein ( hs-CRP) and troponin I( cTnI) in patients with diastolic heart failure.Methods 92 patients with diastolic heart failure were randomly divided into the two groups,the control group and the observation group,46 cases in each group.The control group used the conventional treatment and the observation group on the basis of conventional therapy plus al-prostadil.Two groups were treated for 4 weeks.They were measured filling velocity ratio (E/A) in patients with early and late peak atrial contraction,stroke volume (SV),left ventricular end-diastolic diameter (LVEDD),6min walking distance and serum hs-CRP, cTnI before and after treatment.Results There were no significant difference about LVEDD,E/A and SV(t=0.,0.261,0.445,all P>0.05).After treatment for four weeks,LVEDD were (53.1 ± 5.1)mm and (47.3 ±4.7)mm,LVEDD of the two groups had significantly decreased (t=4.601,9.331,P<0.05, P<0.01),and post-treatment the observation group LVEDD decrease more significantly (t=2.914,P<0.05).After 4 weeks of treatment,the control group after treatment E/A and SV were (0.79 ±0.15) and (72.6 ±5.4),the ob-servation group E/A and SV were (0.85 ±0.14) and (79.5 ±5.9)mL,E/A and SV in both groups were significant-ly elevated (t=3.172,4.710,3.924,5.776,P<0.05,P<0.01),and E/A and SV of post-treatment the observation group increased more significantly (t=3.771,2.840,P<0.05).After treatment,patients 6min walking distance were (383.4 ±47.5)m and (426.1 ±49.1) m,6min walking distance of patients were significantly improved after treat-ment (t=8.776,12.648,P<0.01),and the observation group improved significantly more than that of the control group (t=3.019 2,P<0.05).In the control group,serum hs-CRP patients,cTnI were (21.3 ±4.6) mg/L and (2.13 ±0.52) ng/mL,(13.8 ±4.1) mg/L and (1.05 ±0.38) ng/mL in the observation group.Two groups of patients before treatment serum hs-CRP and cTnI was no significant difference (t=0.527,0.493,all P>0.05).After 4 weeks of treatment,the serum hs-CRP,cTnI were significantly lower in both groups (t=3.612,8.772,2.924, 5.164,P<0.05,P<0.01),and compared with the control,the observation group reduced more significantly (t=3.061,7.114,P<0.05).Conclusion Alprostadil can decrease serum hs-CRP and cTnI of patients with diastolic heart failure.

Abstract Objective: To prepare high-purification and high-specific activity of recombinant human interleukin-6 (rhIL-6). Methods: The rhIL-6 was obstained from inclusion body expressed by IPTG-induced pT7.7hIL-6 expressed vector using extracting,denature and refolding techniques. The rhTL-6 was further purified by anionic-exchange chromatography. Activity of rhIL-6 was measured by 3H-TdR method. Results: After a single-step purification,the product purity reach 95% and it's specific activity was 3.0 x 10~8 U/mg. Conclusion:This scheme of puri-fication was an easy way requiring rhTL-6.

Objective:To observe the effect of Livostin spray on children＇s allergic rhinitis and to search the mechanism of treating allergic rhinitis. Method: 113 patients were treated with Livostin spray (Livostin group) or normal saline spray (control group). Result:The total efficiency of Livostin group in treating allergic rhinitis is above 95.1％ and that of the control group is 25.0％. Initial time of starting effect of Livostin (72.1％) is in 1 minute, and that of the control group (mostly 23.1％) is in 3 minutes. The keeping curativeeffect time of Livostin spray is mostly (72.1％) above 5 hours and that of the control group is mostly (30.8％) in 3 hours. After 2 weeks,the eosinophilic granulocyte number in nose＇s secretion of Livostin group is obviously reduced (P＜0.05). Conclusion: Livostin is better than control group in relieving symptoms, keeping curative effect and safety,so Livostin is one kind of effective drug in treating children＇s allergic rhinitis.

ObjectiveThis study was to evaluate the effects of different pneumoperitoneum used in laparoscopy on in vitro tumor cells growth. Methods 3H-TdR incorporation was used to compare the DNA synthesis (CPM value) of tumor cells after exposure to simulated laparoscopic environments, composed of carbon dioxide, helium and room air, respectively. Results At 24 hours after pneumoperitoneum, compared with group of helium, air and control, CPM increased in group of carbon dioxide (F=47.576 5,P0.05). At 48,72 and 96 hours, pneumoperitoneum, compared with helium group, air group and control group, CPM increased significantly in carbon dioxide group (F=116.183,1082.92 and 5116.75, P0.05). Conclusion Carbon dioxide promotes tumor cells growth, while helium restrains the growth of tumor cells. [

Objective To construct prokaryotic expression plasmid of human recombinant soluble TNF-related apoptosis inducing ligand(rsTRAIL),then to investigate the effects of rsTRAIL on apoptosis in non-small cell lung carcinoma(NSCLC).Methods The encoding sequence for rsTRAIL was amplified with RT-PCR and cloned into PQE30 vector to establish the prokaryotic expression system.The competent cells of host strain of M15 were transformed by the recombinant plasmid.The expression of the target protein was induced with IPTG and purified by Ni2+-NTA agarose column.rsTRAIL was added in the media of A549 and H460wt cells,then the viability was examined by MTT assay.Apoptosis of H460wt cells was observed under fluorescence microscopy.The apoptotic rate of tumor cells was examined by FACS.Results The cloned fragment of rsTRAIL was 100% consistent with that reported in GenBank.The expressed protein with molecular weight of 21 000 in SDS-PAGE as expected was obtained and recognized by a commercial McAb.The apoptotic rate of H460wt cells after treated with rsTRAIL for 24 h was 43.2%.Cislatin enhanced the effect of rsTRAIL on A549 cells.Conclusion The rsTRAIL is obtained after Ni affinity chromatograph.rsTRAIL has a strong cytotoxic activity against NSCLC and cisplatin may enhance the antitumor effect of rsTRAIL.