Objective To explore the possibility of united test of CA72-4, CA19-9 in gastric juice in diagnosis of gastric carcinoma.Methods The gastric juices were respectively obtained by liquid collector made by ourselves through gastreecope in study group(30 cases of gastric cancer) and control groups(32 cases of gastratrophia,30 cases of gastric ulcer,and 30 cases of chronic superficial gastritis).The gastric juice content of CA72-4, CA19-9 were determined synchronously with ES-300 auto-enzymoimmuno-analyzer.Results The gastric juice contents of CA72-4,CA19-9 were (83.54±143.70) U/ml and (47.05±29.64) U/ml, and the positive rates were 76.66% and 43.33% in study group,respectively,and all higher than those of control groups(P <0.01 ).The level of CA72-4 was high in two of 32 patients with gastratrophia,and one was diagnosed as gastric carcinoma after nine months follow-up.Conclusion The combined detection of CA72-4 and CA19-9 provide main evidence for the diagnosis of gastric cancer and diagnostric significance of advanced gastric cancer.

In observing the therapeutic efficacy of insulin sensitizer in treatment of early T2DM,120 T2DM patients were randomly divided into 3 groups: group A received 4 mg rosiglitazone daily;group B received 1.5 g metformin daily;group C received 4 mg rosiglitazone and 1.0 g met-formin every day.Before and after the treatment,fasting glucose,HbA1c,weight,adiponectin,BMI and HOMA-IR were measured or calculated.We found that fasting glucose,HbA1c and HOMA-IR of 3 groups were obviously decreased in comparison with their baseline and adiponectin was significantly increased.In conclusion,insulin sensitizer can obviously decrease the levels of glucose,HbA1c and HOMA-IR,and increase the level of serum adiponectin in T2DM.Insulin sensitizer in combination of metformin is suitable for treatment of T2DM.

Objective To comprehend the prevalence rate,awareness rate and the risk factors of hypertension among inhabitants in Shanxi Province. Methods With cluster random sampling,41 committees and villages among nine areas of Shanxi Province were selected as the investigative spots,including Taiyuan,Datong,Jinzhong,Yuncheng and etc. In each spot,permanent residents aged above 15 yrs were investigated on the correlation study of hypertension. Results In this study,10 792 people were investigated,male 4 721 and female 6 071. The prevalent rate of hypertension was 25.4%,the standardized rate was 23.6%,28.0% in male and 23.4% in female. The awareness rate for hypertension was 44.8%,38.6% in male and 50.5% in female. The multivariate Logistic regression analysis showed the risk factors for hypertension in this population included male,the aged people,lacking of knowledge,drinking alcohol,and obesity. Conclusion There is high prevalent rate of hypertension in Shanxi Province. So we should take the measures aiming at the present situation and risk factors of hypertension.

The clinical probation of medical students in the clinics of gynecology and obstetrics is significant. However, because of the disease involving in female genital system, clinical clerkship teaching in obstetrics and gynecology faces actual problems such as poor cases, low cooperation of the patients and the risk of offending the privacy of patients. During the period of clinical novitiate, two or three teaching clinics were established by experienced clinical teachers, and the patients for the teach-ing were recruited by a series of incentives, such as exempt registration fee, prior doctors' office visit-ing, preferential examine, appointing well-known experts and advanced hospital admission and so on. And the teaching was preceded after the patients' informed consents. As results, the cooperation of the patients was obviously improved, and case selection was made easy, and the outpatient clinic probation teaching level of obstetrics and gynecologyn was raised.

Problem-based learning(PBL)plays an improtant role in fostering the ability of problem-solving,creative thinking and active learning of medical undergraduates.Based on the present clinical education resources,how to implement PBL at the largest advantages is the main issue we should concern.

Objective To investigate the latest situation in the period 2006-2008 of drug resistance tuberculosis among migratory population in Chaoyang district of Beijing.Methods All sputum culture-positive specimens from migratory population were collected.Drug susceptibility of Mycobacterium tuberculosis to M,H,R and E were tested by proportion method.Results Drug susceptibility of 371 isolates from 2006-2008 were tested.Total drug resistance wag 22.4%(83/371),the primary drug resistance and the acquired drug resistance were 17.5%(58/331)and 62.5%(25/40)respectively.The multi-drug resistance was 5.9%(22/371);the primary multi-drug resistance was 4.2%(14/331)and acquired multi-drug resistance wag 20.0%(8/40).The drug resistance and multi-drug resistance were higher in treated cases than that in new cases.and the difference wag statistically significant(χ~2 was 39.020 and 13.210,P<0.01).The drug resistance to 4 anti-tuberculosis drugs from high to low was M(16.7%).H(12.1%),R(11.3%),E(1.9%).The primary drug resistance rate were 13.3%(S),8.8%(H),8.2%(R),1.8%(E).The acquired drug resistance rate were 45.5%(S),40.0%(H),37.5%(R),2.5%(E).The drug resistance to S,H and R were higher in treated cases than that in new cases,and the difference was statistically significant(χ~2 was 23.549,29.810 and 27.754,P<0.01).Conclusion The drug resistance to mycobacterium tuberculosis among migratory population was relatively high in chaoyang district of Beijing,suggesting the necessity to strength the tuberculosis control program for migratory population.

Objective To study the effect of authorization theory on patients with new-diagnosed type 2 diabetes with impaired glucose regulation.Methods Authorization education on diabetes were carried out in 54 new-dhgnosed type 2 diabetes patients with impaired glucose regulation for 1 year.The blood glucose,body weight,BML,systolic and diastolic blood pressure,waist circumference,waist-hip ratio and adiponectin were examined before and after authorization education.Courses of education were divided into five steps:identify the problem;express feelings;Set goals;make a plan;evaluate the result.Results All the above factors alleviated after authorization education,P＜0.05.Conclusions Authorization education for patients can make them change their living style actively and achieve the goal of behavior change.

Abstract] Objective To establish an assay for the detection of porcine parvovirus ( PPV) and to verify its application for monitoring cells used for production.Methods A pair of primers and one probe were designed according to the conserved sequence encoding non-structural protein 1 (NS1).Based on the designed primers, a real-time fluorescent quantitative PCR assay for the detection of PPV was developed. Several parameters including the linearity, precision, minimum detection limit and anti-interference of the established assay were evaluated.A stock of PPV strains was prepared by infecting swine testicle ( ST) cells with PPV strains.An assay for the detection of PPV infection was developed by using ST cells as sensitive cells.A combined ST cell infection-PCR test was developed by combining the ST cell infection assay with the real-time fluorescent quantitative PCR assay.The sensitivity of ST cell infection-PCR test was analyzed.The cell samples used for production of biological products were detected by using the established assay.Results The real-time fluorescent quantitative PCR assay was specific for the detection of PPV without cross-reaction to other species of parvovirus virus, SV40 virus and other porcine viruses.The linear range of the assay was 1×109-1×104 copies/μl with a R2 value more than 0.98.The sensitivity of the real-time quantitative PCR assay was 1×104 copies/μl.Both of the intra-and inter-coefficient of variation (CV) were less than 5%in Ct values.The intra-and inter-CV in copies of detection were 5%-15% and 30%-40% respectively.The minimum detection limit of the real-time fluorescent quantitative PCR assay was 1CCID50/ml.The PPV strains were detected in cell samples with no interference.The sensitivity of ST cells infection-PCR test was 0.01CCID50/ml.All of the 22 cell samples were negative for PPV by using the real-time fluorescent quanti-tative PCR assay.Conclusion The real-time fluorescent quantitative PCR and the ST cell infection-PCR test for the detection of PPV in cells were established successfully.The application of the two assays was conducive to further enhance the safety of using cells for production and therapy.

AIM: To study the electrophysiological effects of nitric oxide (NO) on the pacemaker cells in guinea-pig left ventricular outflow tract under the condition of ischemia/reperfusion ( I/R) .METHODS:The spontaneous slow action potentials of guinea-pig left ventricular outflow tract were recorded by conventional intracellular microelectrode technique.The effects of NO donor sodium nitroprusside ( SNP) on the spontaneous slow action potentials under normal or I/R condition were investigated.RESULTS:SNP at concentrations of 1, 10 and 100μmol/L but not 1000μmol/L signif-icantly increased velocity of diastolic depolarization ( VDD) and rate of pacemaker firing ( RPF) , SNP at concentrations of 1, 10, 100 and 1 000μmol/L notably increased maximal diastolic potential (MDP), amplitude of action potential (APA) and maximal rate of depolarization ( Vmax ) , shortened 50%and 90%of the duration ( APD50 and APD90 ) .In ischemia 10 min group, VDD and RPF were significantly decreased, APA and Vmax were notably increased, and APD50 and APD90 were markedly lengthened compared with control group.In reperfusion 10 min group, VDD and RPF were significantly in-creased, MDP and APA were notably decreased, and APD50 and APD90 were markedly shortened compared with I 10 min group.In reperfusin 10 min group, the pacemaker activity was always irregular.In reperfusion 10 min group, the parame-ters of spontaneous slow action potentials restored to the levels of control group except for VDD and Vmax .In 1, 10 and 100 μmol/L SNP+R groups, VDD and RPF were significantly increased than in ischemia 10 min group .In 1, 10, 100 and 1 000 μmol/L SNP+R groups, APA, APD50 and APD90 were restored to the levels of control group.CONCLUSION:SNP significantly increases the spontaneous activity of left ventricular outflow tract, and relieves the effects of I/R on the sponta-neous slow action potential markedly.

The histopathological changs of the dog eyes caused by acute and subacute toxic effectsof hematoporphyrin monoether--PsD-044 were observed in this research. The results showed thatPsD-044 could lead to keratoscleritis and scleral perivasculitis. Phototoxic cyclitis and inflammatory edema of the retina were more serious in deferred killed animals, suggesting that it is necessary to avoid exposure to light after the administration of PsD-044.