Objective To explore the possibility of united test of CA72-4, CA19-9 in gastric juice in diagnosis of gastric carcinoma.Methods The gastric juices were respectively obtained by liquid collector made by ourselves through gastreecope in study group(30 cases of gastric cancer) and control groups(32 cases of gastratrophia,30 cases of gastric ulcer,and 30 cases of chronic superficial gastritis).The gastric juice content of CA72-4, CA19-9 were determined synchronously with ES-300 auto-enzymoimmuno-analyzer.Results The gastric juice contents of CA72-4,CA19-9 were (83.54±143.70) U/ml and (47.05±29.64) U/ml, and the positive rates were 76.66% and 43.33% in study group,respectively,and all higher than those of control groups(P <0.01 ).The level of CA72-4 was high in two of 32 patients with gastratrophia,and one was diagnosed as gastric carcinoma after nine months follow-up.Conclusion The combined detection of CA72-4 and CA19-9 provide main evidence for the diagnosis of gastric cancer and diagnostric significance of advanced gastric cancer.

Objective To comprehend the prevalence rate,awareness rate and the risk factors of hypertension among inhabitants in Shanxi Province. Methods With cluster random sampling,41 committees and villages among nine areas of Shanxi Province were selected as the investigative spots,including Taiyuan,Datong,Jinzhong,Yuncheng and etc. In each spot,permanent residents aged above 15 yrs were investigated on the correlation study of hypertension. Results In this study,10 792 people were investigated,male 4 721 and female 6 071. The prevalent rate of hypertension was 25.4%,the standardized rate was 23.6%,28.0% in male and 23.4% in female. The awareness rate for hypertension was 44.8%,38.6% in male and 50.5% in female. The multivariate Logistic regression analysis showed the risk factors for hypertension in this population included male,the aged people,lacking of knowledge,drinking alcohol,and obesity. Conclusion There is high prevalent rate of hypertension in Shanxi Province. So we should take the measures aiming at the present situation and risk factors of hypertension.

In observing the therapeutic efficacy of insulin sensitizer in treatment of early T2DM,120 T2DM patients were randomly divided into 3 groups: group A received 4 mg rosiglitazone daily;group B received 1.5 g metformin daily;group C received 4 mg rosiglitazone and 1.0 g met-formin every day.Before and after the treatment,fasting glucose,HbA1c,weight,adiponectin,BMI and HOMA-IR were measured or calculated.We found that fasting glucose,HbA1c and HOMA-IR of 3 groups were obviously decreased in comparison with their baseline and adiponectin was significantly increased.In conclusion,insulin sensitizer can obviously decrease the levels of glucose,HbA1c and HOMA-IR,and increase the level of serum adiponectin in T2DM.Insulin sensitizer in combination of metformin is suitable for treatment of T2DM.

The clinical probation of medical students in the clinics of gynecology and obstetrics is significant. However, because of the disease involving in female genital system, clinical clerkship teaching in obstetrics and gynecology faces actual problems such as poor cases, low cooperation of the patients and the risk of offending the privacy of patients. During the period of clinical novitiate, two or three teaching clinics were established by experienced clinical teachers, and the patients for the teach-ing were recruited by a series of incentives, such as exempt registration fee, prior doctors' office visit-ing, preferential examine, appointing well-known experts and advanced hospital admission and so on. And the teaching was preceded after the patients' informed consents. As results, the cooperation of the patients was obviously improved, and case selection was made easy, and the outpatient clinic probation teaching level of obstetrics and gynecologyn was raised.

Problem-based learning(PBL)plays an improtant role in fostering the ability of problem-solving,creative thinking and active learning of medical undergraduates.Based on the present clinical education resources,how to implement PBL at the largest advantages is the main issue we should concern.

Objective:To provide the basic data for the further revision of mycoplasma test method described in Chinese Pharma-copoeia and some references for the operation standardization of drug mycoplasma test. Methods:Two incubation conditions,namely aerobic conditions and microaerophilic conditions,were compared with respect to the growth status of mycoplasma in liquid and solid media. Results:The growth of mycoplasma was obvious difference between the two incubation conditions,and the microaerophilic con-ditions were better than the aerobic conditions. Conclusion:The microaerophilic conditions can be used in the incubation of mycoplas-ma test,which should be defined and standardized in the future Chinese Pharmacopoeia.

Objective:To investigate the growth-promoting properties and applicability of the mycoplasma test culture media pre-scribed in Chinese pharmacopoeia, and provide reference for the standardization of the drug mycoplasma test method. Methods: Re-spectively using four quantitative detection methods including sensitivity, degree of color change, colony counts and colony diameter, and mycoplasma media widely used in the world as the reference media, the growth-promoting properties of 4 batches of mycoplasma broth and 4 batches of arginine mycoplasma broth from four domestic manufacturers were investigated. Results:The results of sensitivity assay and absorbance detection showed that all the media inoculated with below 100 CFU test microorganisms exhibited visible color change. Furthermore, the results of color change degree and colony diameter showed that there were significant differences among the media products from different manufacturers(P<0. 01). Conclusion:Mycoplasma broth and arginine mycoplasma broth both can sup-port the growth of below 100 CFU test microorganisms. Due to the difference in the growth-promoting properties among the media prod-ucts from different manufacturers, the drug mycoplasma test workers should use more sensitive methods to examine the applicability of the media.

Objective To compare the curative effect of laparoscopic and laparotomic operation for elderly Chinese women with cervical cancer. Methods The randomized controlled trials (RCT) and case controlled trials (CCT) were collected by searches of WanFang database, CNKI, VIP, China Biology Medicine (CBM) , PubMed. Data were extracted from these trials and data analysis was performed by RevMan 5.2.9. Results There were no RCTs, however, a total of 10 CCTs met the inclusion criteria and had data extracted for this review. The baseline characteristics of the laparoscopic group were similar to those of the laparotomic group. Comparing to laparotomic group, the laparoscopic group have longer operation time (MD =32.60, 95%CI: 5.65~59.55, P = 0.020), less amount of bleeding (MD = -94.01, 95%CI: -130.65 ~ -57.37, P = 0.000), smaller number of lymph node dissection(MD = 1.69, 95%CI: 0.67 ~ 2.72, P = 0.001), earlier anus exhaustion (MD=-17.09, 95%CI: -21.19 ~ -12.98, P = 0.000) and shorter hospital stays (MD = -4.30, 95%CI: -5.57 ~ -3.02, P = 0.000). There was significant difference between the two groups. But there was no statistical significance in postoperative indwelling catheter time (MD = -0.67, 95%CI: -1.92 ~ 0.58, P = 0.290) and surgical complications incidence (OR=0.62, 95%CI: 0.27 ~ 1.42, P = 0.260) between the two groups. Conclusion Laparoscopic management has the advantages of less traumatic and recovered quickly, and did not increase the incidence of complications. Laparoscopic operation is an ideal procedure for elderly Chinese women with cervical carcinoma.

AIM: To study the electrophysiological effects of nitric oxide (NO) on the pacemaker cells in guinea-pig left ventricular outflow tract under the condition of ischemia/reperfusion ( I/R) .METHODS:The spontaneous slow action potentials of guinea-pig left ventricular outflow tract were recorded by conventional intracellular microelectrode technique.The effects of NO donor sodium nitroprusside ( SNP) on the spontaneous slow action potentials under normal or I/R condition were investigated.RESULTS:SNP at concentrations of 1, 10 and 100μmol/L but not 1000μmol/L signif-icantly increased velocity of diastolic depolarization ( VDD) and rate of pacemaker firing ( RPF) , SNP at concentrations of 1, 10, 100 and 1 000μmol/L notably increased maximal diastolic potential (MDP), amplitude of action potential (APA) and maximal rate of depolarization ( Vmax ) , shortened 50%and 90%of the duration ( APD50 and APD90 ) .In ischemia 10 min group, VDD and RPF were significantly decreased, APA and Vmax were notably increased, and APD50 and APD90 were markedly lengthened compared with control group.In reperfusion 10 min group, VDD and RPF were significantly in-creased, MDP and APA were notably decreased, and APD50 and APD90 were markedly shortened compared with I 10 min group.In reperfusin 10 min group, the pacemaker activity was always irregular.In reperfusion 10 min group, the parame-ters of spontaneous slow action potentials restored to the levels of control group except for VDD and Vmax .In 1, 10 and 100 μmol/L SNP+R groups, VDD and RPF were significantly increased than in ischemia 10 min group .In 1, 10, 100 and 1 000 μmol/L SNP+R groups, APA, APD50 and APD90 were restored to the levels of control group.CONCLUSION:SNP significantly increases the spontaneous activity of left ventricular outflow tract, and relieves the effects of I/R on the sponta-neous slow action potential markedly.

Abstract] Objective To establish an assay for the detection of porcine parvovirus ( PPV) and to verify its application for monitoring cells used for production.Methods A pair of primers and one probe were designed according to the conserved sequence encoding non-structural protein 1 (NS1).Based on the designed primers, a real-time fluorescent quantitative PCR assay for the detection of PPV was developed. Several parameters including the linearity, precision, minimum detection limit and anti-interference of the established assay were evaluated.A stock of PPV strains was prepared by infecting swine testicle ( ST) cells with PPV strains.An assay for the detection of PPV infection was developed by using ST cells as sensitive cells.A combined ST cell infection-PCR test was developed by combining the ST cell infection assay with the real-time fluorescent quantitative PCR assay.The sensitivity of ST cell infection-PCR test was analyzed.The cell samples used for production of biological products were detected by using the established assay.Results The real-time fluorescent quantitative PCR assay was specific for the detection of PPV without cross-reaction to other species of parvovirus virus, SV40 virus and other porcine viruses.The linear range of the assay was 1×109-1×104 copies/μl with a R2 value more than 0.98.The sensitivity of the real-time quantitative PCR assay was 1×104 copies/μl.Both of the intra-and inter-coefficient of variation (CV) were less than 5%in Ct values.The intra-and inter-CV in copies of detection were 5%-15% and 30%-40% respectively.The minimum detection limit of the real-time fluorescent quantitative PCR assay was 1CCID50/ml.The PPV strains were detected in cell samples with no interference.The sensitivity of ST cells infection-PCR test was 0.01CCID50/ml.All of the 22 cell samples were negative for PPV by using the real-time fluorescent quanti-tative PCR assay.Conclusion The real-time fluorescent quantitative PCR and the ST cell infection-PCR test for the detection of PPV in cells were established successfully.The application of the two assays was conducive to further enhance the safety of using cells for production and therapy.