The retrieval and download of bibliographic information from PubMed were realized using the standard URL interfaces of ESearch and EFetch in E-utilities open programming interface of NCBI.the process and key code can provide reference for searching data from NCBI Database and realizing their deep development and further use.

Objective To compare the clinical effect of the umbilical single hole laparoscopic and traditional three holes laparoscopic surgery in ovarian cyst. Methods 90 patients with ovarian cyst surgery were selected, and ran-domly divided into observation group and control group with 45 patients in each group. The control group was given traditional three holes laparoscopic surgery, and the observation group received single hole laparoscopic surgery. Clinical effect, postoperative complications, ovarian function, aesthetic satisfaction were compared between the two groups. Results The differences of operative time, blood loss, postoperative exhaust time, postoperative hospital stay between the two groups has no statistical difference ( > 0.05), the postoperative pain in 24 h, 72 h in the observa-tion group were significantly lower than that in control group, with significant difference ( < 0.05). There was no significant difference in the incidence of postoperative complications between the two groups ( >0.05). The contents of FSH and LH in the two groups were significantly improved, and the content of E2 was significantly decreased af-ter postoperative 30 days, compared with the preoperative, and the difference were statistically significant ( <0.05). There was no significant difference in LH, FSH and E2 level in the two groups before operation ( >0.05), and there was no significant difference in the content of LH, FSH and E2 between the two groups ( > 0.05). The total satis-faction of the observation group was significantly higher than the control group, and the difference was statistically significant ( <0.05). Conclusion Single hole laparoscopic surgery is adapted to the treatment of patients with ovari-an cyst on the basis of the same effect. Not only reduced the pain, but also improved the patients' satisfaction degree of the incision. It is worthy of clinical promotion.

Objective To study the immunogenicity of extractable nuclear antigens(ENA)in activateed lymphocytes.Methods The ENA of the normal and activated lymphocytes was extracted according to Sharp's method,then syngeneic BALB/C mice were immunized.The dynamic fluctuation of serum IgG anti-dsDNA antibody level in mice was analyzed by ELISA,so did the ENA polypeptide spectrum.The immunofluorescent staining pattern of ANA and renal immunopathologic changes of the mice were investigated.Results ANA could be detected in the sera of the immunized mice by the ENA extracted from the activated lymphocytes,including anti-dsDNA and anti-ENA.The immunofluorescent staining patterns for ANA manifested as homogeneous pattern,peripheral pattern,speckled pattern and nucleolar pattern.Moreover,marked immune complex deposits in glomerulus could be observed in ANA positive mice.The results in those mice immunized by the normal-lymphocyte-ENA were negative.Conclusion The ENA extracted from activated lymphocytes is immunogenic,can drive the production of ANA and cause SLE-like syndrome.

Objective To investigate the value of ultrasound in differential diagnosis of benign and malignant thyroid lesions with rimlike peripheral calcification.Methods Seventy-three patients of thyroid nodules with rimlike peripheral calcification were analyzed retrospectively.All cases were confirmed by surgery and pathology.The efficacy of sonographic features on diagnosis of thyroid nodules was analyzed.Results Among 73 patients,58 (58/73,79.45％) were benign and 15 (15/73,20.55％) were malignant.Among the sonographic features mentioned,the mean size,margin,internal echo and presence of halo showed no significant differences between malignant and benign nodules (all P＞0.05).Proportion of thyroid nodules coexisting with nodular goiter,irregular thickness and interruption of rimlike peripheral calcification had significant differences between malignant and benign nodules (all P＜0.05).The sensitivity,specificity,positive predictive value and negative predictive value of coexisting with nodular goiter for diagnosing benign nodules were 77.59％ (45/58),60.00％ (9/15),88.24 ％ (45/51),40.91％ (9/22),respectively.The sensitivity,specificity,positive predictive value and negative predictive value of irregular thickness for diagnosing malignant nodules were 53.33 ％ (8/15),87.93％ (51/58),53.33％ (8/15),87.93％ (51/58),respectively.The sensitivity,specificity,positive predictive value and negative predictive value of interruption of rimlike peripheral calcification for diagnosing malignant nodules were 73.33％ (11/15),68.97％ (40/58),37.93％ (11/29),90.91％ (40/44),respectively.Conclusion Ultrasonography is helpful to diagnosis of thyroid nodules with rimlike peripheral calcification.Irregular thickness and interruption of calcification are associated with malignancy.

Objective To observe the effects of dexmedetomidine (DEX) on the expressions of TNF-α, IL-1β and apoptosis-related proteins in rat mesenteric artery.Methods Male SD rats of SPF grade were sacrificed and the mesenteric artery was separated under the stereo-microscope.We established an experimental model of vascular injury induced by lipopolysaccharide (LPS) and randomly divided the injured vessels into dexmedetomidine treatment group and control group.DEX treatment group was divided into 10-8, 10-7, and 10-6 mol/L subgroups according to the different concentrations of DEX.RNA and total protein was extracted in each group.The mRNA expressions of TNF-α, IL-1β and CaSR were detected by RT-PCR and the protein expression of TNF-α, Caspase-3 and AMPK were tested by Western blot.Results DEX (10-8, 10-7, and 10-6mol/L) obviously reduced vascular inflammatory reaction induced by lipopolysaccharide, reduced the mRNA and protein expressions of TNF-α as well as mRNA expression of IL-1β.Caspase 3 protein expression significantly lowered in blood vessels in DEX group compared with LPS group.DEX had no obvious effect on lipopolysaccharide-induced vascular AMPK and CaSR mRNA or protein expressions.Conclusion DEX obviously deceased the expressions of inflammation-related proteins, suggesting that DEX has anti-inflammatory effects.

Abstract] Objective To establish an assay for the detection of porcine parvovirus ( PPV) and to verify its application for monitoring cells used for production.Methods A pair of primers and one probe were designed according to the conserved sequence encoding non-structural protein 1 (NS1).Based on the designed primers, a real-time fluorescent quantitative PCR assay for the detection of PPV was developed. Several parameters including the linearity, precision, minimum detection limit and anti-interference of the established assay were evaluated.A stock of PPV strains was prepared by infecting swine testicle ( ST) cells with PPV strains.An assay for the detection of PPV infection was developed by using ST cells as sensitive cells.A combined ST cell infection-PCR test was developed by combining the ST cell infection assay with the real-time fluorescent quantitative PCR assay.The sensitivity of ST cell infection-PCR test was analyzed.The cell samples used for production of biological products were detected by using the established assay.Results The real-time fluorescent quantitative PCR assay was specific for the detection of PPV without cross-reaction to other species of parvovirus virus, SV40 virus and other porcine viruses.The linear range of the assay was 1×109-1×104 copies/μl with a R2 value more than 0.98.The sensitivity of the real-time quantitative PCR assay was 1×104 copies/μl.Both of the intra-and inter-coefficient of variation (CV) were less than 5%in Ct values.The intra-and inter-CV in copies of detection were 5%-15% and 30%-40% respectively.The minimum detection limit of the real-time fluorescent quantitative PCR assay was 1CCID50/ml.The PPV strains were detected in cell samples with no interference.The sensitivity of ST cells infection-PCR test was 0.01CCID50/ml.All of the 22 cell samples were negative for PPV by using the real-time fluorescent quanti-tative PCR assay.Conclusion The real-time fluorescent quantitative PCR and the ST cell infection-PCR test for the detection of PPV in cells were established successfully.The application of the two assays was conducive to further enhance the safety of using cells for production and therapy.

Objective To study the effect of human prolactin (PRL) on total IgG and anti-dsDNA antibody production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE). Methods PBMC from SLE patients and control subjects were cultured, with the stimulation of PRL, interleukin 2 (IL-2), interleukin 10 (IL-10) and phytohemagglutinin (PHA). 3H-thymidine (3H-TdR) incorporation assay was used to study the proliferation of PBMC. Total IgG and anti-dsDNA antibodies in the cultured supernatants were measured by enzyme-linked immunosorbent assay (ELISA) in 19 patients of SLE and in 6 control subjects. Results ①The proliferation of PBMC in vitro was enhanced by 10-9 mol/L PRL in 19 patients of SLE. ②The production of IgG and anti-dsDNA antibodies in PBMC from 10 active SLE patients was much higher than that of 9 inactive patients (P

Objective:To observe any effect of function-oriented training on the balance of recovering stroke survivors and on their ability in the activities of daily living.Methods:Sixty stroke survivors in recovery were randomly divided into an observation group ( n=30) and a control group ( n=30). The control group received routine rehabilitation training, while the observation group underwent function-oriented training for 6 weeks. Before and after the intervention, both groups were evaluated using the Berg balance scale (BBS), the Fugl-Meyer lower extremity assessment (FMA-L), the timed up-and-go test (TUGT) and the modified Barthel index (MBI). Results:After the treatment, significant improvement was observed in all of the measurements in both groups. The improvements in the average BBS, FMA-L and MBI scores and in the TUGT times of the observation group were significantly better than those of the control group.Conclusion:Function-oriented training can improve the balance and the ability in the activities of daily living of stroke survivors more effectively than the routine therapy.

ObjectiveTo explore the factors associated with activity of daily living (ADL) in patients injured from earthquake and to observe the rehabilitation nursing of fractures for them. Methods367 patients were reported and analyzed with Logistic regression. Results and ConclusionThe age (OR=1.016), renal failure (OR=4.648), lower limb fractures (OR=2.404), spine fracture (OR=3.558), right hand fractures (OR=1.190), right foot fractures (OR=4.389) and the complex fractures (OR=2.600) were associated with the poor ADL (<60). The rehabilitation nursing for fracture were reported and discussed.

Objective To study the profiling and authentication of human cell lines in cell bank of our department using short tandem repeat (STR) loci and to analyze the situation of cell contamination and misjudgment. Methods Sixty-one human cells including cells collected and preserved by our cell bank and cells from the other departments were detected by the 16 STR loci-method. To analyze the cross contamina-tion between human cell lines, the results were aligned with profiles published by international cell banks. Isoenzyme detection was employed to authenticate the cell species when the STR signal can not be detected. Results Among the 61 cells, specific profiles were produced by 41 ceils and there was no cross contamina-tion. Thirty-six cells had the completely same STR profiles in 9 STR loci with the same cells preserved by ATCC or JCRB, while 5 cells have different profiling just in the vWA loci. The cells referred above can be recognized as correct cells; Eleven cells (18.0%) were the false cells. Among them, cancer cells of tongue named Tca8113 and cancer cell of liver named HHCC(changed to FHCC98 now) had the same profile with HeLa and HeLa S3 respectively; Two ceils both named HUT-102 have the completely different profiles with ATCC; The signal of 4 cells was not be detected, and all of them were determined as hamster cell lines by u-sing isoenzyme detection. Also 2 cells were identified to be mixed cells. Conclusion The phenomenon of cell misjudgment and cross contamination between cells is serious. Authentication of cell lines correctly, es-pecially for the re-authenticatian of domestic self-established cells, is very important for the guarantee of the reliability and reproducibility for scientific researches.