Objective Ganglouyuchuang lotion was used to dress the wound for Anal fistula in Crohn's disease, control inflammation and promote wound healing.This paper studied the extraction techonology of ganglouyuchuang lotion.Methods Four factors of ganglouyuchuang lotion, including volume of water, extraction time, extraction times and liquid ratio, were studied by the orthogonal test, and three levels were selected for each factor.The content of sodium danshensu, the active component contained in Chinese herbal medicine, was regarded as evaluating indicator, and the content of Danshensu Sodium was determined by HPLC.The water extraction and alcohol precipitation technology of ganglouyuchuang lotion was optimized according to the results of measurement.Results The optimum extraction technology of ganglouyuchuang lotion was as follows: four herbs, including Salvia miltiorrhiza, Radix Astragali, Radix sanguisorbae and Senecio were added with8 times amount of water overnight and decocted 3 times with 2 h, 1.5 h, and 1.5 h respectively, and then the extraction was concentrated to the ratio of herbs and concentrate of 1∶1.5.The results showed that the contents of Danshensu Sodium from the three examples were 0.520, 0.498, and 0.521 mg/mL, and the RSD were 0.34%, 0.41%, and 0.29%.Conclusion The optimum extraction technology is feasible and applicable for the preparation of ganglouyuchuang lotion.

Objective To investigate the molecular mechanism of Helicobacter pylori (H.pylori) resistance to clarithromycin. Methods The E test was used to determine clarithromycin resistant strains of H.pylori , and PCR Restriction Fragment Length Polymorphism (RFLP) analysis for 23S rRNA domain V gene mutations. Results Of nine clarithromycin resistant stains of H.pylori , including six primary and three acquired resistant strains, eight were found to have an A to G mutation in 23S rRNA domain V at position 2144. Conclusions The results indicated that the majority (88.8%) of clarithromycin resistant isolates of H.pylori in Shanghai have an A2144G mutation in 23S rRNA domain V.

Objective To investigate whether a novel mucosal adjuvant (DNA containing six base pair motifs consisting of an unmethylated CpG dinucleotide flanked by two 5′ purines and two 3′ pyrimidines, CpG Oligodeoxynucleotide, CpG ODN),which has not been shown to have significant toxicity,could be an ideal mucosal adjuvant for the development of a H. pylori vaccine in mice model. Methods C57BL/6 mice were orally or intranasally immunized with H. pylori whole cell sonicate(WCS) / cholera toxin (CT) or WCS /CpG ODN, and the corresponding control groups were set. Mice were dosed once a week for four weeks. One week after the last immunization, all animals were challenged by live H. pylori (5?10 8) three times in a five day duration. Two and 8 weeks after the last challenge, all animals were sacrificed to examine infection of H. pylori. Sera, saliva, gastric juice were collected to measure the concentrations of IgG, IgG1, IgG2a and IgA by ELISA. Results The protecting rates against H. pylori infection were 75%(9/12), 70% (7/10) and 0 (0/10) in the group of WCS/CT orally, WCS/CpG ODN intranasally and WCS/CpG ODN orally, respectively. Significantly higher levels of serum IgG2a antibody was found in the group immunized with WCS plus CpG ODN than those found in the sham immunized controls ( P

Metformin is a first-line drug for the treatment of type 2 diabetes mellitus. Recently, some clinical studies have been published reporting a reduced incidence of various neoplastic disease (eg. breast cancer, endometrial cancer and gastrointestinal cancer) in diabetic or nondiabetic patients treated with metformin. Metformin inhibits the mammalian target of rapamycin by activating liver kinase B1 (LKB1)/adenosine 5'-monophosphate-activated protein kinase (AMPK) or decreases blood insulin levels, resulting in inhibition of cancer cell growth. There are also many other mechanisms involved in anti-tumor effect of metformin. Although metformin has significant effect on cancers, the prospects for it as an alternative treatment modality and mechanism in various kinds of tumors need further research.

Objective To apply the oral gastrointestinal ultrasound contrast agents for evaluating the gastric motility abnor‐mality in the patients with anxiety disorder .Methods Twenty patients with anxiety disorder complicating upper digestive tract symptoms without organic pathological changes were enrolled as the anxiety disorder group .Twenty healthy volunteers were en‐rolled as the control group in this study .The two groups orally took gastrointestinal ultrasound contrast agents .The antral contrac‐tion frequency ,antral contraction amplitude amd MI were measured at each time point .GER was calculated .The the gastric motility parameters in the patients with anxiety disorder were evaluated .Results The antral contraction frequency and MI at initial 2 min had no statistical difference between the anxiety group and the control group .The antral contraction amplitude ,antral contraction frequency amd MI at each time point during 5 -10 min after contrast in the anxiety disorder group were significantly decreased compared with the control group ,and the differences were statistically significant (P< 0 .05) .After 20 min ,GER in the control group was significantly higher than that in the anxiety group ,the difference was statistically significant (P<0 .05) .Conclusion O‐ral gastrointestinal ultrasound contrast agents is a economic ,strongly operable ,non‐invasive and highly repeatable method for evalu‐ating the gastric motility .

Adenoma, Pleomorphic ; diagnosis ; pathology ; Humans ; Trachea ; pathology ; Tracheal Neoplasms ; diagnosis ; pathology

Objective To investigate the suppression effect of expressing parvovirus H-1 nonstructural protein 1 (NS1) gene on human gastric cancer cells and the possible mechanisms.Methods A recombinant enhanced green fluorescent protein (eGFP) labeled NS1 of parvovirus H-1 plasmid was constructed.Human gastric cancer cell line SGC7901 was transfected with recombinant plasmid (experiment group) or blank vector (negative control group) and blank control group was treated with equal amount of phosphate buffered saline (blank control group).After transfection,the distribution of fluorescent signal was observed under fluorescent microscope.The expression of NS1 at gene and protein level was measured.Cell growth curve of each group was drawn.The expression of cell senescence-associated β-galactosidase (SA-β-Gal) was tested.The changes of cell cycle were investigated by flowcytometry.Two groups' comparision was performed by t-test.Results After transfection,NS1 was expressed in SGC7901 cells at gene and protein level.Compared with negative control group,the fluorescent signal accumulated in cell nucleus in experiment group.The percentage of SA-β-Gal positive cell in experiment group ((30.5 ± 1.4) ％) was higher than that of negative control group ((4.4± 1.1) ％) and the difference was statistically significant (t =-12.931,P ＜ 0.01).The growth inhibition rate of SGC7901 cells from the first day to the fourth day was 45％,62％,73％ and 77％,respectively.The cell cycle of eGFP-NS1 expressed SGC7901 cells was arrested at G0/G1 phase.Conclusion Parvovirus H-1 NS1 play the role in cell nucleus of gastric cancer cell line SGC7901 and could make cell cycle arrested at G0/G1 phase,which effectively inhibited the proliferation SGC7901 cell.

Objective Xiangqingzhitong spray ( XQ spray) is a kind of class V new Chinese drugs .This paper studied the anti-inflammatory and analgesic effects of XQ spray to provide experiment evidence for clinical application . Methods Mice were divided into blank control A group , yunnan baiyao aerosol A group , voltaren A group and low , middle and high dose A group of XQ spray.Analgesic effects were studied by observing effect of XQ spray for pain threshold in electrical stimulation test .Mice were divided into blank control B group , yunnan baiyao aerosol B group , voltaren B group and low , middle and high dose B group of XQ spray .Analgesic effects were studied by observing the effects of XQ spray for pain threshold in tail pain tenderness test .Mice were divided into blank control C group , yunnan baiyao aerosol C group , voltaren C group and low,middle and high dose C group of XQ spray .Anti-inflammatory effects were observed by the models of ear swelling induced by xylene in mice.Rats were divided into blank control D group , yunnan baiyao aerosol D group , voltaren D group and low, middle and high dose D group of XQ spray .Anti-inflammatory effects were observed by the models of paw edema induced by carrageenin in rats . Results Electrical stimulation test showed that middle and high dose A group of XQ spray could enhance the pain threshold and the analgesia percentage reached 90%after the administration of 15 min and 30 min.Compared with control group , significant difference was found in medium and high dose A group (P<0.05).High dose B group of XQ spray could enhance pain threshold in tail pain tenderness test markedly after the administration of 15 min and 30 min and the difference was significant compared with control group ( P<0.01).Middle dose C group could inhibit the ear swelling induced by xylene in mice and the difference was significant compared with control group ([4.41 ±4.55]mg vs [9.15 ±3.94]mg,P<0.05).High dose C group could inhibit the ear swelling induced by xylene in mice and significant difference was found compared with control group ([3.62 ±3.56]mg vs [9.15 ±3.94] mg,P<0.01). High dose D group could inhibit the paw edema induced by carrageenin in rats after the administration of 30 min and there was signifi-cant difference compared with control group ([1.19 ±0.94]mg vs [2.54 ±1.28]mg,P<0.01). Conclusion XQ spray has sig-nificant anti-inflammatory and analgesic effects .

OBJECTIVE: This study was conducted to evaluate the percutaneous absorption of aconitine and mesaconitine in extracts of Radix Aconitum kusnezoffii. METHODS: The extracts of Radix Aconitum kusnezoffii were collected by using Franz diffusion cells after permeation through the skin of rats. Then rate constants of skin permeation of aconitine and mesaconitine were determined by high-performance liquid chromatography (HPLC). RESULTS: Under the condition that the concentrations of azone and propylene glycol were both 4%, the cumulative doses of skin permeation (Q) of mesaconitine and aconitine in the extracts of Radix Aconitum kusnezoffii (600 mg/ml) for 24 hours were 165.819 and 487.747 microg/cm(2) respectively, and their rate constants of skin permeation were 18.391 and 78.805 microg.cm(-2).h(-1) respectively. CONCLUSION: The aconitine and mesaconitine in the extracts of Radix Aconitum kusnezoffii can penetrate well through the skin of rats. Propylene glycol and azone can promote this penetration effects. The formula of skin permeation of mesaconitine and aconitine is in accordance with Higuchi equation and there is a linear relationship between Q and t(1/2).

Objective To evaluate the influences of femoral rotation on dynamic alignment of the lower extremity in TKA by computer assisted surgical technology.Methods From July 2013 to August 2013,76 consecutive patients with knee osteoarthritis undergone primary-single TKA in our hospital were enrolled,including 46 males and 30 females,aged from 64 to 78 years (average,71 years).All surgeries were completed by the same professional doctor.The PFC (fixed-bearing posterior stabilized) Sigma rotating-platform was selected for all patients,and computer navigation system (Stryker(R) Navigation System Ⅱ-Cart (Stryker corporation,Kalamazoo,Michigan,USA) was used.External rotation osteotomy of the distal femur during TKA was completed according to the pre-operative external rotation angle (ERA),intraoperative transepicondylar axis (TEA) and anteroposterior (AP) line.Passive dynamic alignment of the lower extremity during knee flexion was recorded.The variation trend of hip-knee-ankle (HKA) alignment and the influences of femoral external rotation osteotomy were analysed.Results ERA calculated by the computer navigation system according to Whiteside line,TEA and the mean of Whiteside line and TEA was 5.54°±2.86°,5.21°±2.60° and 5.34°±2.38°.Compared with the ERA measured by CT,no method was better than the other two.Postoperative deviation of HKA alignment from 0° to 90° flexion was associated with the rotational alignment of the femoral component.Trend of HKA alignment during knee flexion tended to be varus,valgus or neutral according to different angles of external rotation osteotomy of the distal femur.Conclusion The HKA alignment changes during the knee flexions.External rotation osteotomy of the distal femur played a crucial role in determining dynamic HKA alignment in TKA.