Objective To determine the Golgi dispersal in radiation damaged cells and the protective effect of vanillin derivatives.Methods Immunofluorescence, cell cycle analysis of flow-cytometry,Western blot,and clone formation were used.Results Immunofluorescence observation showed that the Golgi dispersal caused by 2 Gy 60 Coγ-ray was significantly increased in a dose-dependent manner in the range of 4-10 Gy as was demonstrated by the fact that the Golgi area was significantly increased. When the irradiated cells were treated with the radioprotective agent VND3207, a vanillin derivative,the Golgi dispersal induced by radiation was significantly reduced.The radiation-induced Golgi dispersal was also displayed in a pattern of time-course after irradiation in the HeLa cells, and persisted at least to 36 h post-irradiation. Cell cycle test results indicated that the Golgi dispersal was not associated with the G2/M arrest triggered by radiation-induced DNA damage response.VND3207 could promote cell survival by plate colony formation assay.Conclusion The Golgi dispersal can be caused byγ-ray irradiation in a dose-and time-dependent manner, and VND3207 can provide a good protection against radiation injury associated with inhibited Golgi dispersal.

Objective To define the effect and mechanism of hyperkalemic solution on atrial natriuretic peptide (ANP) secretion in rabbits. Methods Eighteen rabbits were selected and the chest was opened under anes-thetization to remove the heart. The left atrium was isolated and fixed in the atrial perfusion system with proper electric stimulation for beating. The following experiments were carried out on beating rabbit atria: ①The atrium was perfused for 60 min to stabilize parameters of ANP secretion and atrial dynamics. The control period (12 min as an experimental cycle) was followed by an infusion of hyperkalemic solution (K+ concentration of hyperkalemic solution was 5.64 mmol/L and the osmolarity of hyperkalemic solution was unchanged) for three cycles, then normal K+ cancentration was recovered for two cycles;②The control period was followed by an infusion of L type Ca2+ channel blocker nifedipine (1.0 μmol/L) for three cycles;③L type Ca2+ channel inhibitor nifedipine (1.0 μmol/L) was infused for 36 rain prior (three cycles) to infusion of hyperkalemic solution. Atrial stroke volume was determined and the ANP secretion was measured by radioimmtmoaasay. Results (1)Hyperkalemic solution increased atrial ANP secretion (P<0.01) and reduced the atrial stroke volume,hut the difference was not statistically significant as compared with that of the control cycle(P>0.05). The recovery trend was to the normal level of ANP secretion and atrial stroke volume was to become normal gradually when solution level recovered to normal ,which was not significantly different from that of the control cycle (P>0.05) ;②Nifedipine (1.0 μmol/L) also increased the atrial ANP secretion (P<0.01 or P <0.05) while decreasing atrial stroke volume (P<0.01 or P < 0.05 ) ; ③Nifodipine (1.0μmol/L) completely blocked the effect of hyperkalemic solution so to increase the ANP secretion (P <0.01 ). Conclusion Hyperkalemic solution significantly increases atrial ANP secretion via extracellular high K+ competitive inhibition of extracellular Ca2+ inflow in beating rabbit atria.

Objective To establish an HPLC method for the determination of anthraquinones including rhein, emodin and chrysophanol in xinshenyan capsules. Methods Anthraquinones were determined by HPLC with Phenomenex-C18 column (250 mm×4. 6 mm, 5 μm) as the chromatographic column and methanol-1% acetic acid (70:30) as the mobile phase. The flow rate was 1. 0 mL·min-1 and the detection wavelength was set at 254 nm. Results The liner range of rhein, emodin and chrysophanol was 4. 96-24. 80 μg·mL-1(r=0. 999 6), 6. 58-32. 91 μg·mL-1(r=0. 999 9) ,and 15. 11-75. 55 μg·mL-1 (r=0. 999 9),respectively, and the average recovery was 100. 78%, 98. 13% and 99. 29%, respectively. Conclusion The method is simple and practical, the result is accurate and reliable and it can be used to determine the contents of rhein, emodin and chrysophanol in xinshenyan capsules.

Objective Former standards for Yishen Pills only identify angelica root , astragalus root and ligustrum lucidum by thin layer chromatography ( TLC) and there is no test for the content of effective components .The study was to improve the quality of Yishen Pills by perfecting the quality standards . Methods We determined the content of specnuezhenide in Yishen Pills by high-per-formance liquid chromatography(HPLC), with a mobile phase of MeOH-Water, a chromatography column of Agilent TC-C18(2) (4.6 × 250 mm, 5μm), a flow rate of 1.0 mL/min, a column temperature of 25℃, a detection wavelength of 224 nm, and a sample loop volume of 10μL. Results The linear relationship of specnuezhenide content was good in the range of 15.375μg/mL~246.000μg/mL.The relative standard deviation of precision experiment , stablity experiment and repeatablity experiment was 0.44%, 0.95%and 2.65%re-spectively.The average recovery was 99.60%.The qualified standard for specnuezhenide in Yishen Pills was ≥0.3 mg/g. Conclusion The method is simple , accurate and reliable , with good reproducibility , and it is applicable for the quality control of Yishen Pills .

Objective To establish method of the determination of aloe-emodin ,rhein ,emodin ,chrysophanol and physcion in Xinbaoshen tablet ,for the production of quality protection .Methods HPLC was performed on a Lichrospher-C18 column (250 mm × 4 .6mm ,5 μm)with the mobile phase of methanol-0 .1% H3 PO4 (70:30) .The flow rate was 1 .0 ml/min ,the col-umn temperature was at 35 ℃ and the detection wavelength was 254 nm .Results The linear rang of aloe-emodin ,rhein ,emod-in ,chrysophanol and physcion were obtained between 2 .30-18 .4 μg/ml ( r= 1 .0) ,2 .930-23 .44 μg/ml( r= 1 .0) ,5 .00-40 .0 μg/ml ( r=1 .0) ,14 .870-118 .96μg/ml( r= 0 .9998 ) and 7 .410-59 .28 μg/ml(r=0 .999 9) ,the average recoveries were 100 .16% ,102 .91% ,99 .76% ,100 .32% ,100 .44% ,RSD were 1 .58% ,1 .27% ,1 .67% ,1 .33% ,1 .03% (n=9) .Conclusion The improved method was accurate and feasible which could be used convenient in quality control .