AIM:To investigate the regulation of miR-222 on BCL2L13 gene and its effect on the growth and apoptosis of HBx-HepG2 cells, and to explore the underlying molecular mechanisms .METHODS:The expression level of miR-222 was detected by RT-qPCR.The HBx-HepG2 cell growth was examined by MTT and colony formation assays .The cell cycle and apoptosis were analyzed by flow cytometry .The recombination vector pmirGLO-BCL2L13 was constructed, and dual-luciferase reporter experiment was performed to validate the target of miR-222.RESULTS:The expression level of miR-222 in the HBx-HepG2 cells was significantly higher than that in the L 02 cells ( P<0.05 ) .Over-expression of miR-222 enhanced HBx-HepG2 cell growth, changed cell cycle, and inhibited apoptosis (P<0.05).Knockdown of miR-222 reduced HBx-HepG2 cell growth, changed cell cycle, and increased cell apoptotic rate (P<0.05).BCL2L13 was down-regulated in the HBx-HepG2 cells as compared with L02 cells (P<0.05), and knockdown of miR-222 in the HBx-HepG2 cells increased the expression level of BCL2L13 (P<0.05).The results of dual-luciferase reporter assay and re-store experiment showed that miR-222 negatively regulated the expression of BCL2L13 via targeting 3’ UTR of BCL2L13, resulting in the promotion of HBx-HepG2 cell growth .CONCLUSION: miR-222 enhances HBx-HepG2 cell growth via down-regulation of BCL2L13.

Objective By using CiteSpace-based visualization analysis to investigate the main research contents,research frontiers,author cooperation network and institutional cooperation network of traditional Chinese medicine(TCM)treatment for patients after receiving percutaneous coronary intervention(PCI)so as to provide reference for the TCM diagnosis,treatment and research of patients after receiving PCI.Methods A computerized retrieval of academic papers concerning TCM treatment of patients after receiving PCI from CNKI,VIP and Wanfang databases was conducted.The retrieved literature materials were input into NoteExpress software to make the literature screening and the data cleaning.CiteSpace software was used to analyze the key words,authors,institutions,etc.Results A total of 1 708 articles were obtained;the number of published articles in 2020 was the largest,which was up to 137 articles.The core authors accounted for 4.37％of the total number of authors who had written articles,but no core author group had been formed yet.Liaoning province of China was the region where the number of published articles was the largest,and there was no effective collaboration network between different areas.The cluster analysis yielded 25 clusters,448 key nodes and 1 266 lines.The main cluster results were coronary heart disease,acute coronary syndrome,acute myocardial infarction,restenosis,etc.The analysis of emergent words(i.e.the suddenly increased frequency of a certain key word in a certain period)yielded 24 emergent words,including syndrome differentiation of TCM,ginseng peony oral liquid,cardiac rehabilitation,etc.Conclusion The main research contents of TCM treatment for patients after PCI include syndrome differentiation of TCM,etiology and pathogenesis,clinical trials,etc.Postoperative pathological phenomena such as restenosis are the research difficulties in this field,and anxiety,dual-mind TCM,and cardiac rehabilitation are the current research hotspots.TCM has definite efficacy in promoting the recovery of cardiac function after PCI,in reducing the incidence of postoperative events such as restenosis,and in improving anxiety and depression.

Objective? To explore the effect of evidence-based cancer pain nursing on cancer pain patients. Methods? Using convenient sampling method, 83 patients with cancer and pain who were diagnosed and treated in the First People′s Hospital of Chun′an County, Zhejiang Province from March 2014 to September 2016 were selected as the study objects. According to the random number table method, they were divided into control group (n=41) and observation group (n=42). The control group adopted the routine nursing mode, and the observation group adopted the evidence-based cancer pain nursing based on the routine nursing. Visual Analogue Scale (VAS), Self-rating Anxiety Scale(SAS), Self-rating Depression Scale(SDS), Piper Fatigue Scale (PFS) were used to evaluate the pain and psychological status of the two groups, and to analyze the improvement of cancer-related fatigue in the two groups. Results? Before intervention, there was no significant difference between the two groups (P＞0.05); after intervention, the VAS, SAS, SDS and cancer-related fatigue scores in the observation group were lower than those in the control group with statistical significance (P＜ 0.05). Conclusions? Evidence-based pain nursing intervention for cancer pain patients can relieve the pain and mental state of cancer pain patients, reduce cancer-related fatigue, and improve the quality of life of patients.

Objective To investigate the predictive value of the C-reactive protein to albumin ratio(CAR)combined with grip strength and serum prealbumin for survival and malnutritionin pa-tients with esophageal cancer.Methods A total of 212 patients with esophageal cancer were selected as study objects,and baseline characteristics,pretreatment CAR,grip strength,and serum prealbu-min results were collected.Nutritional status was assessed,and overall survival was followed up.Re-ceiver operating characteristic(ROC)curves were used to evaluate the predictive value of pretreatment CAR,grip strength,and serum prealbumin for malnutrition.Kaplan-Meier survival curves and Cox re-gression models were employed to analyze their predictive value for survival outcomes.Results ROC curve analysis revealed that grip strength had the largest area under the curve(0.625)for predicting malnutritionin esophageal cancer patients,followed by serum prealbumin and CAR(0.604,0.594).Based on the Youden index,the optimal cut-off values for CAR,grip strength,and serum prealbumin were 0.732,23.1 kg,and 0.190 g/L,respectively.The sensitivity of the combined detection of three indicators was 80.7％.Kaplan-Meier survival curve analysis showed that patients with elevated CAR(≥0.732),reduced grip strength(＜23.1 kg),and decreased serum prealbumin levels(＜0.190 g/L)had shorter overall survival compared to those with CAR＜0.732,grip strength ≥23.1 kg,and serum prealbumin levels ≥0.190 g/L,respectively(P＜0.01).Multivariate Cox regression analysis identi-fied elevated pretreatment CAR(≥0.732),reduced grip strength(＜23.1 kg),and decreased ser-um prealbumin levels(＜0.190 g/L)as independent risk factors for poor survival prognosis in esoph-ageal cancer patients(P＜0.05).Conclusion Pretreatment CAR,grip strength,and serum preal-bumin are important indicators for assessing nutritional status and survival prognosis in esophageal cancer patients.Combined detection of these biomarkers may facilitate early diagnosis and timely in-tervene malnutrition,potentially improving survival outcomes in esophageal cancer patients.

Objective To investigate the predictive value of the C-reactive protein to albumin ratio(CAR)combined with grip strength and serum prealbumin for survival and malnutritionin pa-tients with esophageal cancer.Methods A total of 212 patients with esophageal cancer were selected as study objects,and baseline characteristics,pretreatment CAR,grip strength,and serum prealbu-min results were collected.Nutritional status was assessed,and overall survival was followed up.Re-ceiver operating characteristic(ROC)curves were used to evaluate the predictive value of pretreatment CAR,grip strength,and serum prealbumin for malnutrition.Kaplan-Meier survival curves and Cox re-gression models were employed to analyze their predictive value for survival outcomes.Results ROC curve analysis revealed that grip strength had the largest area under the curve(0.625)for predicting malnutritionin esophageal cancer patients,followed by serum prealbumin and CAR(0.604,0.594).Based on the Youden index,the optimal cut-off values for CAR,grip strength,and serum prealbumin were 0.732,23.1 kg,and 0.190 g/L,respectively.The sensitivity of the combined detection of three indicators was 80.7％.Kaplan-Meier survival curve analysis showed that patients with elevated CAR(≥0.732),reduced grip strength(＜23.1 kg),and decreased serum prealbumin levels(＜0.190 g/L)had shorter overall survival compared to those with CAR＜0.732,grip strength ≥23.1 kg,and serum prealbumin levels ≥0.190 g/L,respectively(P＜0.01).Multivariate Cox regression analysis identi-fied elevated pretreatment CAR(≥0.732),reduced grip strength(＜23.1 kg),and decreased ser-um prealbumin levels(＜0.190 g/L)as independent risk factors for poor survival prognosis in esoph-ageal cancer patients(P＜0.05).Conclusion Pretreatment CAR,grip strength,and serum preal-bumin are important indicators for assessing nutritional status and survival prognosis in esophageal cancer patients.Combined detection of these biomarkers may facilitate early diagnosis and timely in-tervene malnutrition,potentially improving survival outcomes in esophageal cancer patients.

The pathogenesis of nonalcoholic fatty liver disease (NAFLD) remains unclear, and currently no effective drugs have been approved for the treatment of NAFLD. Polygonum cuspidatum is a natural traditional Chinese medicine with a long history of application, and studies have shown that it plays an important role in the treatment of NAFLD. This article summarizes related research findings in the active components of Polygonum cuspidatum applied in the treatment of NAFLD, and it is found that the active components of Polygonum cuspidatum can improve insulin resistance, exert an anti-oxidative stress effect, regulate lipid metabolism, improve endoplasmic reticulum stress, and alleviate inflammatory infiltration by regulating the signaling pathways including Nrf2, AMPK, NF-κB, SIRT1, and PPARα, thereby exerting a preventive and therapeutic effect on NAFLD, so as to provide a basis and ideas for developing drugs for NAFLD and exploring related mechanisms.