Salivary gland polyt-ne chromosome was studied in malarial vtctor Anopheles messeae Fall, 1926 of Xinjiang Uygar Zizhiqu.Photograph and diagram of polytene chromosomal karyotype were presented.Four types of intraspecific polymorphic paracentric inversion were found.They were heterozygous inversions in arm 2R and 3R,heterozygous inversion in 3L and its homozygous form.No obvious difference of polytene chromosomal bands was found between Xinjiang An.messeae and Soviet Union An.messeae and they shared the same polymorphic inversion form, but different inversion frequency.

Smear method was used to study the mitotic chromosomal karyotype and heterochromatin-banding of Anopheles messeae in Xinjiang Uygar Zizhiqu of north-west China. Intraspecific polymorphisms were found in sex chromosome. It is explained that these polymorphisms were formed by variation in heterochromatin quantity and distribution. The result suggests that chromosomal karyotype and banding are valuable in anopheline identification especially in species complex.

Mitotic chromosomal karyotypes and their heterochromatin banding of thirteen anopheline mosquitoes in China were observed. Five species belonging to subgenus Cellia were Anopheles maculatus, An. dints, An. kochi, An. splendidus and An. minimus. Eight species belonging to subgenus Anopheles were An. barbirostris, An. messeae, An. crawfordi, An. kunmingensis, An. kweiyangensis, An, hyrcanus, An. sinensis and An. anthropophagus. The results show that the interspecific differences of sex chromosomes and heterochromatin differences in autosomes are useful in anopheline sibling species identification. Two types of completely different chromosomal karyotypes of An. maculatus were found from Yunnan and Sichuan Provinces, and two types of obviously different heterochromatin banding of An. dims were found from Hainan and Yunnan Provinces. Thus, An. maculatus and An. dirus are respectively sibling species complex in China. The salivary gland polytene chromosomes of An. hyrcanus, An. sinensis and An. anthropophagus were also studied. The authors find that the main differ ences of polytene chromosomes of the three species are fixed paracentric inversions in arm 2L.

Using scanning electromicroscope, seven species of anopheline mosquito eggs were studied. Among them, the microstructure of exochorion of Anopheles liangshanensis. An. kweiyangensis An. kunmingensis, An. hyrcanus and An. messeae were not reported before. The results showed that ultrastructure of plastron network, frill and tubercles of deck were useful in distinguishing sibling species of anopheline eggs. Microstructure of float had little difference between species. The micropylar area and lobed tubercles presented obvious intraspecific variations, so, it should be careful for using in classification.

The zymograms of EST in six species of anopheline mosquitoes (Female) were studied with polyacrylamide gel electrophoresis.The zymograms of EST were divided into three groups,EST Ⅰ, EST Ⅱ and EST Ⅲ, respectively.The Rm of subgenus Anopheles (including An sinensis,An.anthropophagus and An.kunmigensis) was larger than that of subgenus Cellia (including An.dirus, An.mmmus and An.kochi) in main bands of EST Ⅲ. The results showed specific characteristics at the taxonomic level of subgenus. The six species of anopheline mosquitoes could be differentiated from each other by the particular band pattern and Rm in EST n groups.

1.00) than the subgenus Cellia ( An.kochi.An.maculanis, An.dirus. An. jeyporiensis, and An.mininus) with Rm value