Objective: To analyse the fatty acid and trace element composition and antioxidant capacity of Trionyx sinensis. [WT5FZ]Methods:[WT5BZ] We determined the contents of fatty acids and trace elements in Trionyx sinensis with gas chromatography and atomic absorption spectrometry respectively. The rats were fed on a diet containing 0.8% Trionyx sinensis powder. The activities of GSH Px and SOD in blood, SOD in liver, CAT in muscle and the contents of MDA in serum and liver were determined. [WT5FZ]Results:[WT5BZ] We found that the contents of MUFA, Zn and Se were higher in Trionyx sinensis. The activities of GSH Px and SOD in blood, SOD in liver, CAT in muscle were increased, but the contents of MDA in serum and liver were decreased in rats. [WT5FZ]Conclusion:[WT5BX] Trionyx sinensis can increase the activity of antioxidant enzymes in rats and lower the content of lipid preoxides.

C57/B6J mice were fixed in traditional fixative (4% formaldehyde solution , the same below) and novel fixative respectively , and the routine procedure of paraffin section preparation was performed , as well as the novel fixative was validated in HE⁃stained paraffin sections of the eyes of mice and human embryos in the age⁃related macular degeneration (AMD) model. Compared with the traditional fixative , the quality of the paraffin sections treated with the novel fixative was better than that of the traditional fixative , and successfully simulated the changes of the retinal layers of AMD mice at different time periods. The novel fixative is feasible for the preparation of high⁃quality retinal paraffin sections , which lays the foundation for subsequent studies on retinal paraffin sections.