1.Clinical observation on therapeutic effect of integrated traditional Chinese and western medicine for treatment of severe acute pancreatitis and nursing
Shuangzhen YUAN ; Lixian ZHANG ; Wei LI ; Man DONG ; Jichao LU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2017;24(2):205-206
2.Gastric cancer stem cells in tumor invasion and metastasis and its influence on angiogenesis ability
Lixian ZHANG ; Ning ZHANG ; Shuangzhen YUAN ; Yumei CHEN ; Xinai SU ; Jianshun SUN ; Lihua WANG ; Mei LIN
Chinese Journal of Tissue Engineering Research 2016;20(32):4738-4744
BACKGROUND:Gastric cancer stem cels involved in the neoadjuvant chemotherapy and conventional treatment are closely associated with relapse of gastric cancer. However, this conclusion has not yet been confirmed.
OBJECTIVE:To investigate the effects of gastric cancer stem cels in tumor invasion and metastasis and its effect on angiogenesis ability.
METHODS:We prepared nude mouse models of gastric cancer to isolate and culture gastric cancer stem cels. Harvested gastric cancer stem cels were detected in cel scratch test, ring test, inhibition rate test, cel migration test and tumorigenicity test.
RESULTS AND CONCLUSION:After 7 days of culture, the cels exhibited adherent growth but a lack of regularity that most cels were in a tadpole shape. In the cel scratch test, the scratch width was significantly different at 0 and 24 hours (P < 0.05). Under an inverted microscope, the cels were found to form a ring in the ring test. The 50% inhibiting concentration of gastric cancer stem cels induced by oxaliplatin was significantly lower than that induced by 5-fluorouracil (P < 0.05). The number of cels passing through the basilar membrane was significantly increased after cel migration (P < 0.05). After implantation of gastric cancer stem cels, the gastric tissue quality was significantly higher than that in normal nude mice of gastric cancer (P < 0.05). These findings indicate that gastric cancer stem cels responsible for tumor invasion and migration have stronger angiogenesis ability.
3. Effect of diary psychological guidance on negative emotions and self-efficacy of patients with duodenal ulcer
Penglu GUO ; Shuangzhen YUAN ; Wei LI ; Man DONG ; Jichao LU ; Chengyun LANG
Chinese Journal of Practical Nursing 2019;35(14):1094-1098
Objective:
To analyze the effect of diary psychological guidance on negative emotion and self-efficacy in patients with duodenal ulcer.
Methods:
From August 2016 to September 2018, 88 patients with duodenal bulbar ulcer admitted to Harrison International Peace Hospital were divided into study group and control group by random grouping method, 44 cases in each group. The control group was given traditional intervention, while the study group was given diary psychological guidance on the basis of the control group. Both groups were assessed by SAS and SDS, Pittsburgh Sleep Scale, SF-36 Quality of Life Scale and Self-efficacy Scale before and after intervention at the end of the second week.
Results:
After intervention, SAS and SDS of the study group were (51.3±2.0), (52.4±1.9) respectively, while those of the control group were (58.5±2.8), (59.3±3.1). There were significant differences between the two groups (