1.Simultaneous determination of thirteen saponins in Shusanqi Powder by HPLC
Zexuan YU ; Xiangxiang DAI ; Shuangyou DU ; Rengang MAO ; Xianrong WU
Chinese Traditional Patent Medicine 2017;39(6):1179-1182
AIM To develop an HPLC method for the simultaneous content determination of ginsenosides Rb1,Rd,F4,Rg1,20 (R)-Rg3,20 (S)-Rg3,Rgs,20 (R)-Rh1,20 (S)-Rh1,Rh4,Rk1,Rk3 and notoginsenoside R1 in Shusanqi Powder (processed Notoginseng Radix et Rhizoma).METHODS The analysis of 70% methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorabax-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 203 nm.RESULTS Thirteen saponins showed good linear relationships within their own ranges (r =0.999 5),whose average recoveries were 90.01%-108.32% with the RSDs of 0.62%-3.54%.CONCLUSION This sensitive and accurate method can be used for the quality control of Shusanqi Powder.
2.Preparation of ebastine dispersible tablets by hot-melt dispersion
Chengzhi XU ; Zhiliang ZHAO ; Shuangyou DU ; Qianjian FENG ; Chong ZHANG
Journal of Pharmaceutical Practice 2017;35(5):415-418,471
Objective To prepare ebastine dispersible tablets by hot-melt dispersion method using hypromellose as a carrier.Methods Ebastine was heated to melt and dispersed in hot solution of hypromellose.The mixture was adsorbed by mannitol and then used to prepare dispersible tablets.The characteristics of hot-melt dispersion were analyzed by the methods of differential scanning calorimetry and X-ray powder diffraction.A comparison of dissolution curves between self-made dispersible tablets and original ebastine tablets was performed.Results The analysis of X-ray diffraction and differential scanning calorimetry showed that the ebastine in solid matrix remained the main crystalline characteristics.The dissolution of ebastine hot-melt dispersion was 95.07% in 15 min in vitro, which was significantly higher than that of micronized ebastine raw material.Moreover, the accumulative dissolution of ebastine dispersible tablets in 30 min was about 30% higher than the original drug.Conclusion The method of hot-melt dispersion using hypromellose as a carrier improved the ebastine dissolution significantly.