Objective Using the technology of siRNA to inhibit gene expression of T cells'nonreceptor tyrosine protein kinase Lck in asthmatic mice,and to study the effect of siRNA inhibited Lck to the function of T cells in asthmatic mice.Methods The 21 - 23 bp RNA fragments of mouse T cell Lck were made by chemosynthesis.INTERFERinTMsiRNA Transfection Reagent was used as transfection reagent to transfect the siRNA into the spleen T cells of asthmatic mice for 48 hours.Then T cells were mixed with bone marrow dendritic cells (DC) of asthmatic mice for another 48 hours.Cell culture suspension was collected and the level of IL-4,IL-13,IL-2,INF-γ were detected with respondent ELISA kits; Western Blot was used to identify if the expression of Lck was blocked.Results The expression of Lck in T cells almost could not be detected in siRNA interference group.The levels of IL-4 and IL-13 in siRNA interference group( 10.19 ± 1.66,12.34 ±0.79) were lower than no-siRNA interference(28.06 ±2.88,27.87 ± 1.61 )and control group ( 22.07 ± 2.5 1,20.47 ± 2.37 ),and the difference was statistical significant ( P ＜0.01 ).Conclusions Special siRNA could block the expression of special gene,and Lck specific siRNA could block the activation and differentiation of T cells and reduce the secretion of inflammatory cytokines in asthmatic mice.

ObjectiveUsing the technology of siRNA to inhibit the gene expression of no-receptor　tyrosine protein kinase Lck in T cells of asthmatic mice,and to study the therapeutic effect of Lck specific　siRNA in asthmatic mice.MethodsReceptor tyrosine protein kinase Lck specific siRNA fragments were　taken from chemosynthesis.In vivo-jetPEITM was used to transfect the siRNA into mice body through tail　vein injection.The mice were killed 48 hours later,and the levels of IL-4,IL-17 in bronchoalveolar lavage　fluid (BALF) were detected with respondent ELISA kits.The change of inflammatory histopathology in lung　was observed with H.E.staining.The expression of Lck in lung was detected with immunohistochemistry　(IHC),and the level of Lck in lung tissue homogenate was detected with Western Blot.Results Compared with asthmatic group[ (234.68 ± 11.15 ) pg/ml,( 96.76 ± 8.28 ) pg/ml],the levels of IL-4,IL-17　[ (234.68 ± 11.15)pg/ml,(96.76 ±8.28) pg/ml] in the BALF of siRNA interference group decreased,　and the inflammation in the lung relieved.IHC indicated that the expression of Lck in lung decreased and　the level of Lck in lung tissue homogenate decreased ( P ＜ 0.05 ).Conclusions Lck specific siRNA　could reduce the level of IL-4,IL-17 in the lung tissues of asthmatic mice,and relieve the inflammatory reaction in lung.

In recent years, the clinical guidelines for the diagnosis and treatment of rheumatoid arthritis (RA) have been constantly updated. Among the general principles, it is particularly emphasized that, in order to improve the ratio of treat to target(T2T) of RA, doctors and patients should work together to negotiate the details of the guidelines. Therefore, it is important for patients to further understand the disease and clinical guidelines of RA, and to better cooperate with doctors. This study was based on the most concerned issues of RA patients and international standard procedure of guideline study, we organized the working group and introduce the following 16 recommendations constituting the RA patients′ practice guidelines.

BackgroundIndividuals may experience significant alterations in sleep hygiene during the major public health emergencies， consequently impacting their sleep quality. ObjectiveTo explore the relationship between sleep quality and sleep hygiene among adult residents during the major public health emergencies， so as to provide references for improving the sleep quality of residents during such a period. MethodsA sample of 1 364 adult residents were enrolled as the research subjects from February 20 to 29， 2020. All participants were asked to complete self-administered questionnaire to obtain basic-demographic information and sleep hygiene. Pittsburgh Sleep Quality Index （PSQI） was applied to assess sleep quality. Residents were classified into poor sleepers with PSQI score ≥8 and good sleepers defined as PSQI score <8. Binary Logistic regression analysis was conducted to identify factors associated with sleep quality. Radar chart was used to visualize and compare the sleep hygiene between poor sleepers and good sleepers. ResultsAccording to PSQI score， 891 （65.32%） residents were good sleepers， while 473 （34.68%） residents were poor sleepers. Comparison revealed that age （χ²=3.887）， past medical history （χ²=27.938）， awareness rate of importance of sleeping before major public health emergencies （χ²=4.337）， impact of sleep quality on quality of life during the major public health emergencies （χ²=178.138）， frequency of staying up late during the major public health emergencies （χ²=139.390）， compensatory sleep behaviors （χ²=39.257）， impact of sleep problems on daytime functioning （χ²=285.879）， change of bedtime （χ²=63.031）， sleep latency （χ²=168.672）， wake-up time （χ²=59.221）， changes in sleep duration （χ²=172.332）， time spent in the bedroom （χ²=23.071）， and sum of money spent on improving sleep environment （χ²=58.584） yielded statistical difference between poor sleepers and good sleepers （P<0.05 or 0.01）. Logistic regression analysis denoted that past medical history （OR=1.680， 95% CI： 1.185~2.382）， negative impact of sleep quality on quality life （OR=4.181， 95% CI： 2.722~6.422）， staying up late 3 to 4 times per week （OR=3.145， 95% CI： 1.497~6.605）， staying up late almost every day （OR=4.271， 95% CI： 1.970~9.260）， negative impact of sleep problems on daytime functioning （OR=7.169， 95% CI： 5.188~9.907）， prolonged sleep latency （OR=2.836， 95% CI： 2.019~3.982） and shortened sleep duration （OR=3.518， 95% CI： 2.144~5.772） were risk factors of poor sleep quality. The sum of money spent on improving sleep environment following the major public health emergencies ≤500 RMB （OR=0.334， 95% CI： 0.134~0.830） was related to the incidence rate of poor sleep quality. Radar chart showed that poor sleepers were characterized by extravagant concerns， excessive cleanliness and poor sleep hygiene practices during the major public health emergencies， and poor sleepers were more likely to stay up late due to stress and emotional issues. ConclusionSome residents are facing poor sleep quality during the major public health emergencies， and poor sleep hygiene practice also contributes to poor sleep quality.

Objective: To evaluate the value of two oral mucosal contouring methods for predicting acute radiation-induced oral mucositis (A-ROM) in nasopharyngeal carcinoma (NPC) patients. Methods: A total of 150 AJCC 7^th stage Ⅱ-IVB NPCs receiving radical tomotherapy (TOMO) in Zhejiang Cancer Hospital from 2017 to 2019 were included in this prospective observational study. Oral cavity contour (OCC) and mucosal surface contour (MSC) were applied to delineate the oral mucosal structure. A-ROM grade was prospectively assessed and recorded weekly according to RTOG scoring criteria. The prediction value of two methods for A-ROM was statistically compared. Results: The incidence rate of ≥3 grade A-ROM was 33.3%. In univariate analysis, V₅, V₁₀, V₁₅, V₄₅, V₅₀, V₅₅, V₆₀, V₆₅ and V₇₀ of OCC and V₅, V₁₀, V₅₀, V₅₅, V₆₀, V₆₅, V₇₀ and D_mean of MSC were significantly correlated with the risk of ≥3 grade A-ROM (all P<0.05). In binary logistic regression analysis, gender and smoking were significantly associated with the incidence of ≥3 grade A-ROM by using OCC (male vs. female: OR=0.141, 95%CI=0.037-0.538, P=0.004; smoking vs.non-smoking: OR=5.109, 95%CI=1.413-18.470, P=0.013). For MSC, gender, smoking, N stage and MSC- V₅₅ were the independent predictors (male vs. female: OR=0.129, 95%CI=0.032-0.519, P=0.004; smoking vs.non-smoking: OR=4.448, 95%CI=1.224-16.164, P=0.023; N stage: OR=2.291, 95%CI=1.268-4.137, P=0.006; MSC-V₅₅: OR=1.432, 95%CI=1.008-2.033, P=0.045). The cutoff value of MSC-V₅₅ was 7.70%, the area under ROC curve was 0.754, the sensitivity and specificity were 0.680 and 0.740, retrospectively (all P<0.001). Conclusions Compared with OCC, MSC yields a higher prediction accuracy for the severity of A-ROM in nasopharyngeal carcinoma patients receiving TOMO treatment.