Objective Micronucleus test(MNT) was used to evaluate the single and combined genotoxicity of dichlorvos(DDVP),dimethoate(DM) and malathion(Mal) in HepG2 cell line,and 2?2 factorial design was adopted to elucidate the combined genotoxic effects.Methods In cytotoxicity test,HepG2 cells were exposed to the three OPPs(DDCP,DM,Mal) for 4 h respectively,and the doses at which cell viability above 80% were selected for the MNT,DDVP:3.125-25 ?g/ml,DM:25-200 ?g/ml,Mal:50400 ?g/ml,the micronucleated cell(MNC) rates and the replicative index(RI) were calculated.The combined genotoxicity of them was investigated with their doses as follows:low dose(DDVP:3.125 ?g/ml,DM:25 ?g/ml,Mal:50 ?g/ml);high dose(DDVP:12.5 ?g/ml,DM:100 ?g/ml,Mal:200 ?g/ml).Results In MNT,after treatment of HepG2 cells with DDVP,DM or Mal alone for 4 h,the MNC rates were increased in a dose-response manner(DDVP:r=0.955,P

Objective To investigate colonization status and risk factors of multidrug-resistant organisms (MDROs)in a surgical intensive care unit (SICU),and provide a basis for active clinical screening of MDROs. Methods From June 1,2013 to August 31,2013,patients who admitted to SICU≥24 hours were performed active screening,the colnization status of methicillin-resistant Staphylococcus aureus (MRSA)and extended-spectrumβ-lactamase-producing Escherichiacoli/Klebsiellapneumoniae (ESBL-E.coli/Kp)among patients were detected,re-lated risk factors were analyzed. Results When patients who admitted to SICU≤48 hours,the detection rate of MRSA and ESBL-E.coli/Kp was 1 1 .00% and 73 .00% respectively;when admitted to SICU>7 days,the increased detection rate of MRSA and ESBL-E.coli/Kp was 16.67% and 44.44% respectively. Patients stayed in hospital >7 days before admit-ting to SICU (OR95% CI:4.48 [1 .21-16.65 ])was an independent risk factor of carrying MRSA when admitting to SICU,APACHEⅡscore ≥16 (OR95% CI:6.36[1.47-27.54])was an independent risk factor of carrying MRSA 48 hours after admitting to SICU. Conclusion When patients admitted to SICU,the carrying rate of MDROs is high,isola-tion rate rises with prolonged length of SICU stay. Hospitals should carry out MDRO colonization screening proj ect among patients and implement effective isolation control measures to reduce the incidence of healthcare-associated infection.

Objective:To investigate the current situation of sharp instrument injuries among medical workers in a tertiary general hospital in Fuzhou, so as to provide basis for formulating relevant policies.Methods:In June 2019, medical personnel working in a tertiary general hospital in Fuzhou, who may have sharp instrument injuries were selected as the research object. A total of 2720 questionnaires were received, including 2688 valid questionnaires, with an effective rate of 98.8%. The age, type of work, professional title, working years, operating habits, occurrence and reporting of sharp instrument injuries among medical staff from June 2018 to May 2019 were investigated.Results:The incidence of sharp instrument injury was 37.6% (1011/2688) . Among them, 20.6% (208/1011) had multiple sharp instrument injuries. The exposure rate of contaminated sharp instruments was 15.1% (405/2688) . With the increase of age, professional title and working years, the incidence of sharp instrument injury decreased year by year (χ 2trend=12.393, 33.339, 15.160, P<0.05) . The first three causes of sharp instrument injury were breaking glass ampoules by hand (39.1%, 395/1011) , extracting liquid medicine (10.4%, 105/1011) and handling sharp instruments by hand (10.3%, 104/1011) . The main sharp instruments causing sharp injury were ampoules (43.2%, 437/1011) , syringe needles (20.3%, 205/1011) and suture needles (9.6%, 97/1011) . 874 (86.4%) medical staff had missed reports after sharp instrument injuries. Conclusion:The occurrence of sharp instrument injury in this hospital is still serious, and the protection of sharp instrument injury should be strengthened.

Objective:To investigate the current situation of sharp instrument injuries among medical workers in a tertiary general hospital in Fuzhou, so as to provide basis for formulating relevant policies.Methods:In June 2019, medical personnel working in a tertiary general hospital in Fuzhou, who may have sharp instrument injuries were selected as the research object. A total of 2720 questionnaires were received, including 2688 valid questionnaires, with an effective rate of 98.8%. The age, type of work, professional title, working years, operating habits, occurrence and reporting of sharp instrument injuries among medical staff from June 2018 to May 2019 were investigated.Results:The incidence of sharp instrument injury was 37.6% (1011/2688) . Among them, 20.6% (208/1011) had multiple sharp instrument injuries. The exposure rate of contaminated sharp instruments was 15.1% (405/2688) . With the increase of age, professional title and working years, the incidence of sharp instrument injury decreased year by year (χ 2trend=12.393, 33.339, 15.160, P<0.05) . The first three causes of sharp instrument injury were breaking glass ampoules by hand (39.1%, 395/1011) , extracting liquid medicine (10.4%, 105/1011) and handling sharp instruments by hand (10.3%, 104/1011) . The main sharp instruments causing sharp injury were ampoules (43.2%, 437/1011) , syringe needles (20.3%, 205/1011) and suture needles (9.6%, 97/1011) . 874 (86.4%) medical staff had missed reports after sharp instrument injuries. Conclusion:The occurrence of sharp instrument injury in this hospital is still serious, and the protection of sharp instrument injury should be strengthened.

Objective To observe toxic symptoms and signs , toxic damage extents and reversibility in rats after oral administration of Tangwang Mingmu granules .Methods Four dose groups with 40 rats in each group were designed in this study, including control group fed with distilled water and three groups at different dosages of the test drug .Tangwang Mingmu granules were orally administered to SD rats at the dosage of 8.4, 4.2 and 2.1 g/kg for 3 weeks and 14.0, 8.4 and 4.2 g/kg for 23 weeks, for 26 consecutive weeks .The general state of the rats was observed every day , while body mass and food consumption were calculated once a week .Halfway through and at the end of the administration (13 and 26 weeks) and after four weeks of recovery, parameters of body mass, hematology, hematological biochemistry, organ/body mass ratio and histopathology were measured .Results Compared with the control group at the same time-point, body mass of male rats in the other three groups was slightly reduced .Food consumption in high and medium dose groups was reduced (P<0.05), MCHC, ALT, TBIL and Na +in high dose group were decreased (P<0.05), TP, ALB and D-BIL were increased (P<0.05), the mean body mass and relative organ weight of thymus in medium dose male rats were decreased (P<0.05), relative organ weight of the liver and kidney in high dose male rats was increased (P<0.05), and focal chronic inflammation to different extent was observed in the liver , kidney and prostate gland .No dose-effect relationship was found in these perturbations that were all within the normal range of animals .No significant drug-related pathological changes were found.Conclusion The NOAEL of Tangwang Mingmu granules is considered to be 14.0 g/kg body mass/day (equal to 50 times the proposed clinical adult dosage ) for the 26-week repeated dose oral toxicity study in male andfemale rats.