Objective:To simulate the urinary tract environment in vitro and observe the local thermal effects generated by the holmium laser when it is continuously emitting under different mode settings, working medium and perfusion speed.Methods:This study was conducted from March to December 2019. Static medium model: Under normal pressure, 25℃ constant temperature and 50% humidity, a glass test tube with an inner diameter of 1cm and working medium of 1ml was placed into a beaker containing 300ml of 37℃ constant temperature water, and a 550 μm laser fiber was placed in the working medium in the test tube. The laser was continuously emitting for 60.0 seconds, and the temperature of the water in the test tube was continuously recorded using a temperature measuring instrument. The laser working mode was set as dusting, fragmenting, and "popcorn" mode, the energy was set at 10-20 W, and the working medium was normal saline, distilled water, and 5% mannitol solution. Flow medium model: In the same environment, the same optical fiber and temperature probe were placed in a 6mm flush tube, and the laser was continuously fired and continuously perfused with saline. The flushing speed was controlled between 100 ml/h and 1 200 ml/h, and the water temperature change during laser emission was recorded. The real-time temperature changes around the fiber tip with different modes, different media, and different perfusion rates were analyzed.Results:Static medium model: After the holmium laser was continuously fired for about 6.0±1.2 seconds, it exceeded the safe temperature of 43℃ , reached the plateau temperature in about 27.6±2.1 seconds and drop to the safe temperature after stopping excitation for 38.2±2.4 seconds. The plateau temperature dusting group > "popcorn" group > fragmenting group ( P<0.01), of which the dusting group(78.67±0.45)℃ (20 W) was the highest and the fragmenting group (55.67±0.22)℃ (10 W) was the lowest. The temperature of 5% mannitol solution (73.92 ± 0.44) ℃ was the lowest among the three experimental working media, which was (75.57±0.14)℃ of distilled water group and (78.67±0.45)℃ of normal saline group (0.4 J×50 Hz, 20 W). Flow medium model: The perfusion rate of 800ml/h can ensure that the continuous emitting of the holmium laser remains at a safe temperature (40.96±0.36)℃. It only took 7.0±1.0 seconds to decrease to the initial temperature after stopping emittion. Conclusions:Under the same total power, the high-energy-low-frequency fragmenting lithotripsy mode has a relatively lower thermal effect. Under the same total power, the thermal effect of holmium laser emittion is relatively lower in the environment of 5% mannitol solution. Perfusion of ≥800ml/h can effectively reduce the local high temperature caused by the thermal effect of holmium laser.

Objective To investigate the efficacy and safety of radical nephrectomy associated with venous thrombectomy and the role of preoperative angioembolization.Methods From Sep 2006 to Dec 2014,the data from 15 cases with renal cell carcinoma and venous tumor thrombus were collected and analyzed retrospectively.The 15 patients included 8 men and 7 women,whose age ranged from 16 to 75 years.Before operation,all patients underwent imaging examinations which demonstrated the renal tumor and venous thrombus.The tumors size ranged from 5.4 to 14.5 cm.The levels of venous thrombus included 0 grade in 4 cases,Ⅰ grade in 2 cases,Ⅱ grade in 6 cases and Ⅲ grade in 3 cases.The 15 patients were divided into angioembolization group (n =5) and non-angioembolization group (n =10) according to the conduction of preoperative angioembolization.Results All cases successful accepted the nephrectomy.The venous thrombectomy were undergone in 14 cases except for one case due to the severe adhesion between renal vein and aorta.The average operative time was 243.3 ± 77.0 min.The mean blood loss was 1 373.3 ± 1 440.9 ml and the volume of blood transfusion was 533.3 ± 521.9 ml.The average time of postoperative hospital stay was 12.7 ± 5.2 days.Symptomatic tumor thrombus embolism didn't occur in all cases,perioperatively.There were no significant difference between these two groups in operative time,blood loss,blood transfusion volume and postoperative hospital stay (P ＞ 0.05).Eight cases were followed up with a period of 6 to 69 months.Four cases had disease-free survival during follow up.Two cases died at 30 and 55 months after surgery,respectively.One had tumor recurrence at 6 months after surgery.One patient accepted a 6-months target therapy (sunitinib) before surgery.However,his thrombus could not be removed during the operation.After the operation,he continued to choose the target drug therapy for 18 months.No progression for thrombosis or metastasis has been found.Conclusions Nephrectomy and venous thrombectomy could be safe and effective for renal cell carcinoma associated with venous thrombosis.Preoperative angioembolization could not reduce the perioperative risk such as blood loss.

This study was aimed at isolating a peptide with hypoglycemic activity from dried red deer antler. A peptide from dried red deer antler of Tarim in Xingjiang was extracted with acetate buffer(pH 3. 5), followed by precipitation with ethanol, ultrafiltration and separation via ion exchange chromatography, gel filtration and reversed phase chromatography. The molecular mass of the peptide was detected via MALDI TOF/MS and the peptide was identified by LC-MS/MS. The activities of the peptide to promote islet β cells proliferation and recovery the damage induced by streptozotocin(STZ)were detected by MTT, and the effect of the peptide on glucose consumption of hepatocyte was determined. The peptide, named as CAP, was obtained from dried red deer antler through purification with the molecular mass of 6 804 via MALDI TOF/MS. The result of LC-MS/MS and the comparison in relative database showed that CAP was a novel peptide. CAP stimulated the proliferation of pancreatic cells and recovery of STZ-damaged pancreatic cells, as well as increased glucose consumption of HepG2.

This study retrospectively analyzed the clinical data of four patients with malignant tumor-induced ureteral strictures admitted to our hospital. All patients successfully underwent ureteroscopic metal stent placement surgery under real-time ultrasound guidance, with a surgical duration of (64±33) minutes. Postoperatively, there was a creatinine decrease of (74±127) mmol/L. Only two cases experienced Clavien-Dindo grade I complications postoperatively. The stents were well positioned in all patients, with a postoperative hospital stay of (2.5±0.6) days. This surgical approach for malignant tumor-induced ureteral obstruction can compensate for the limitations of endoscopic visualization.