1.Diagnosis of Abdominal Organs Diseases by Contrast-Enhanced Ultrasonography
Zeyuan HE ; Liqiong WANG ; Shuangfeng TANG ; Xiaoyu LI ; Ai HE
Journal of Kunming Medical University 2013;(9):123-125
Objective To investigate the efficacy of contrast-enhanced ultrasound (CEUS) in diagnosing liver, gallbladder, spleen and renal diseases. Methods 27 patients with liver diseases, 30 patients with gallbladder diseases, 5 patients with renal diseases, 5 patients with renal trauma and 6 patients with spleen disease were examined by CEUS. Results There were 3 cases of primary hepatocellular carcinomas, 4 cases of metastatic hepatic carcinomas, 4 cases of liver abscess, 10 cases of hepatic hemangiomas and 3 cases of liver repture with active bleeding. The diagnostic coincidence rate of CEUS was 90%. 30 patients with gallbladder polyposis were diagnosed by CEUS, and the diagnostic coincidence rate was 100%. 5 cases with renal diaseses included 1 of renal abscess, 1 of renal column hypertrophy and 3 of renal hemangioma. In 5 cases with renal trauma, there were 3 cases with renal contusion and 2 cases with renal rupture and active bleeding. The coincidence rate was 61%. In 6 cases with spleen diseases, there were 1 case with splenic infraction, 1 case with splenic abscess and 4 cases with splenic rupture and hemorrhage. The coincidence rate was 100%. Conclusion CEUS has great value of clinical application in diagnosis of liver, gallbladder, spleen and renal diseases.
2.Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system.
Yao MENG ; Jiagui JIN ; Shuangfeng LIU ; Min YANG ; Qinglian ZHANG ; Li WAN ; Kun TANG
Journal of Biomedical Engineering 2014;31(1):136-141
Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). The results showed that the chosen PEG/(NH4)2SO4 ATPS could be affected by PEG molecular weight, pH, concentration of PEG and (NH4)2SO4, and inorganic salt as well as additional amount of crude enzyme. After evaluating these influencing factors, the final optimum purification strategy was formed by 16.5% (m/m) PEG2000, 13.2% (m/m) (NH4)2SO4, pH 7.5 and 30% (m/m) additive crude enzyme, respectively. The NaCl was a negative influencing factor which would lead to lower purification fold and activity recovery. These conditions eventually resulted in the activity recovery of 89% (m/m), distribution coefficient of 1.2 and purification fold of 7.0.
Ammonium Sulfate
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chemistry
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Glycerolphosphate Dehydrogenase
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chemistry
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isolation & purification
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Molecular Weight
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Polyethylene Glycols
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chemistry
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Water
3.Assessment of ergonomic load on the prevalence of work-related musculoskeletal disorders in ICU nurses
Shuangfeng ZHENG ; Yuan YUAN ; Likun LUO ; Ting TANG ; Yinglan LI ; Xin JIANG ; Ping YAN
China Occupational Medicine 2023;50(2):155-158