Objective: To study the effects of TNF, IL-1 and IL-6 on bone resorption in cultured calvaria of newborn mice. Methods: Calvaria of newborn mice was cultured and exposed to TNF, IL-1 or IL-6 at various dosages for 48 hours (treated grooups), or without the above agents(control). Calcium concentration in culture medium was determined by atomic absorption spectrum apparatus. The calcium concentration of the treated groups versus that of the control was used as the absorption index. Results: The absorption indexes in the medium of treated groups were 1.92～3. 14 or 1. 85 ～ 3. 34 when the bone was exposed to TNF or IL-6 at 10 ～ 320 ng/ml, those were 1.36 ～ 2.32 when the bone was exposed to IL-1 at 2～64 ng/ ml. Conclusion: TNF, IL-1 and IL-6 may stimulate bone resorption of calvaria in newborn mice.