A blended readers training model which combines online training (micro-lecture, online class, etc) with traditional training ( face to face teaching plus computer training) was proposed according to the theories in re-lation with blended learning, and scientific strategies for interaction of readers were established, which include the 5 dimensions ( demand, object, content, resource and evaluation) in order to integrate online learning resources and traditional offline training, optimize the allocation of training resources, and improve the training efficiency.

Objective:To obesrve the influence of DNA-PKcs in the chemoresistance of multi-drug resistance malignant glioma cells,and to explore its molecuIar mechanism in chemoresistance.Methods:siRNA was used to construct the DNA-PKcs knockdown human glioma U251 cell line;Western blotting method was used to detect the expressions of DNA-PKcs in U251 cells (U251 cells), doxorubicin (ADM)resistant U251 cells (U251/ADM cells),DNA-PKcs knockdown and ADM resistant U251 cells (U251/ADM/siDNA-PKcs cells).CCK8 method was used to detect the cell proliferation activity in three groups;Western blotting method was used to detect the expressions of MDR1, pNF-κB/p6, total Akt, pAkt/T308 and pAKT/S473 in the cells in three groups. Results:The expression level of DNA-PKcs in U251/ADM cells was significantly higher than those in U251 cells and U251/ADM/siDNA-PKcs cells (P < 0.01 ).The IC50 values of doxorubicin (ADM),paclitaxel (PTX), gemcitabine (GEM)in U251/ADM/siDNA-PKcs cells were significantly lower than that in U251/ADM cells (P <0.05);the expression levels of pAKT/S473,pNF-κB/p65,and MDR1 in U251/ADM/siDNA-PKcs cells were significantly lower than those in U251/ADM cells (P <0.01),but the total Akt and pAkt/T308 had no significant differences between two groups (P >0.05).Conclusion:DNA-PKcs can significantly enhance the chemoresistance of multi-drug resistance malignant glioma cells,the underlying mechanism is related to up-regulation of pAKT/S473,pNF-κB/p65 and MDR1 expressions.

Objective To study the value of CT and MRI(3D heavy T_2WI) in cerebrospinal fluid rhinorrhea.Methods 4 cases with spontaneous cerebrospinal fluid (CSF) rhinorrhea confirmed by operation were collected in this study.There were 2 men and 2 women.All patients underwent CT thickness was 0.625 mm and no gaps.MRI (coronal 3D heavy T2WI,thickness of 1 mm,TR/TE 5000 ms/119 ms).Results All 4 cases accepted the treatment of operations.The defects bone were showed by CT,and the sign of CSF of intracranial cavity communicated with nasal cavity diretly was showed by MRI,pituitary herniated into sphenoid sinus in one case also showed by MRI.Conclusion For spontaneous cerebrospinal fluid rhinorrhea,thin-section coronal CT scan and MR coronal 3D heavy T_2WI can clearly show the location of fistulas.

Toll-like receptor 2 (TLR2) is the most w idely expressed receptor in the Toll-like receptor family, and is also an important pattern recognition receptor of the innate immune system to pathogenic microorganisms. In addition, TLR2 can also identify endogenous risk signals and participate in non -pathogenic microbial inflammatory reaction in ischemic injury. The role of TLR2 and its signal transduction in ischemic cerebral w hite matter lesions have received more and more attention. This article reviews the relationship between TLR2 and ischemic cerebral white matter lesion.

The β-Glucuronidase gene (sbGUS) cDNA firstly from Scutellari abaicalensis leaf was cloned by RT-PCR, with GenBank accession number KR364726. The full length cDNA of sbGUS was 1 584 bp with an open reading frame (ORF), encoding an unstable protein with 527 amino acids. The bioinformatic analysis showed that the sbGUS encoding protein had isoelectric point (pI) of 5.55 and a calculated molecular weight about 58.724 8 kDa, with a transmembrane regions and signal peptide, had conserved domains of glycoside hydrolase super family and unintegrated trans-glycosidase catalytic structure. In the secondary structure, the percentage of alpha helix, extended strand, β-extended and random coil were 25.62%, 28.84%, 13.28% and 32.26%, respectively. The homologous analysis indicated the nucleotide sequence 98.93% similarity and the amino acid sequence 98.29% similarity with S. baicalensis (BAA97804.1), in the nine positions were different. The expression level of sGUS was the highest in root based on a real-time PCR analysis, followed by flower and stem, and the lowest was in stem. The results provide a foundation for exploring the molecular function of sbGUS involved in baicalcin biosynthesis based on synthetic biology approach in S. baicalensis plants.
Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; Computational Biology ; Glucuronidase ; chemistry ; genetics ; metabolism ; Molecular Sequence Data ; Open Reading Frames ; Phylogeny ; Plant Proteins ; chemistry ; genetics ; metabolism ; Protein Structure, Secondary ; Scutellaria baicalensis ; chemistry ; enzymology ; genetics ; Sequence Alignment

Objective To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in pancreatic cancer and its relationship with P-glycoprotein (P-gp) and vascular endothelial growth factor (VEGF),and to study its correlation with invasion,metastasis and drug re- sistance.Methods The expressions of EMMPRIN,P-gp and VEGF in 34 cases of pancreatic cancer were detected by immunohistochemistry.The correlation analysis and Chi squared test were been used by SPSS 10.0.Results The positive rates of EMMPRIN,P-gp and VEGF in pancreatic cancer were 61.8%,55.9% and 64.7% respectively.EMMPRIN,P-gp and VEGF were associated with lymph node metastasis (P＜0.05),but not associated with sex,age,size of tumor and degree of differentia- tion (P＞0.05).There were close relationship between EMMPR1N and P-gp,VEGF respectively (r= 0.398,r=0.432,P＜0.05).Conclusions EMMPRIN,P-gp and VEGF were higher expressed in pancreatic cancer.EMMPRIN express was correlated with both P-gp and VEGF.It has indicated that during the progression of pancreatic cancer,there are close relationship between invasion,metastasis and drug resistance,and EMMPRIN may play key role in this process.

Acute Disease ; Adolescent ; Humans ; Male ; Myositis

Disasters ; Emergency Medical Services ; Humans ; Wounds and Injuries ; therapy

Objective To investigate the effect of nicergoline on cognitive ability and executive function for patients with vascular cognitive impairment without dementia (VCIND).Methods Totally 68 patients with VCIND were divided into conventional treatment group (33 cases) and nicergoline treatment group (35 cases,nicergoline 10 mg,tid added on basal conventional treatment).All of cases were continuously treated for 12 weeks.Cognitive ability and executive function before and after treatments were evaluated.Results Test scores of immediate free recall of a list of words,the numbers and symbols transformation,the word fluency,clocks and watches painting,and minimental state examination (MMSE) were significantly higher in nicergoline treatment group than in conventional treatment group (P＜0.05).Conclusions Nicergoline may improve attention and memory as well as cognitive ability in patients with vascular cognitive impairment without dementia.

Objective To know the microbial pollution in the indoor air of classrooms and dormitories in a university and analyze the possible impact factors.Methods In April and May,2007,the indoor air sampling was conducted and the total count of air microbe in indoors was determined in eight classrooms and twenty dormitories by the impacting method.Results According to the related national hygienic standard of China, the total count of air microbe in all classrooms was qualified, the total count of air microbe in the dormitories was all qualified, but it was higher in male's dormitories compared with female's.Conclusion In general, there was not obviously indoor microbial pollution in the investigated university.