Objective To explore the diagnostic value of serum procallcitonin(PCT)in the diagnosis of prostatitis.Methods 125 cases of patients with bacterial prostatitis,85 cases of patients with non-bacterial prostatitis and 90 cases of healthy individuals were selected as subjects,and serum levels of PCT were detected and compared.The diagnostic efficacy of PCT in the diagnosis of bacterial prostatitis was evaluated as well.Results The serum level of PCT of patients with bacterial prostatitis were higher than those of patients with non-bacterial prostatitis and healthy individuals,had statistically significant differences(P <0.05).The sensi-tivity and specificity of PCT for diagnosing bacterial prostatitis was 72.0% and 89.0%,and PLT had good diagnostic efficacy.Con-clusion Determination of PCT could provide references for early diagnosis and differentiation of PLT,and rational treatment.

Epilepsy is one of the common diseases in the nervous system with its complicated pathogenesis still remains unknown.The drug resistance mechanism of intractable epilepsy has always been a key point in the research of neuroscience.A possible cause for the drug resistance is the over expression of efflux drug transporters,e.g.ATP-binding cassette transporters,which may decrease extracellular antiepileptic drugs levels in brains of intractable epilepsy patients.ATP-binding cassette transporters are super family of transporter proteins that require ATP hydrolysis for the transport of substrates across membranes,including P-glycoprotein,multidrug resistance-associated protein,major vault protein and breast cancer resistance associated protein.They are major impediment for the AED successful treatment of many forms of refractory epilepsy in human.This paper reviews the research progress on over-expression of ATP-binding cassette transporters and mechanism of drug resistance in intractable epilepsy.

Objective: Through analyzing the level, construction and operation efficiency of revenue, to analyze the basic status and change trend of current economics operation of county-level hospitals in this area, provide former basic information for making comprehensive reform evaluation on public hospitals. Methods: To collect data of annual health financial report, collect and analysis the data. Results: The overall level of revenue and expenditure of couty-level hospitals in Hainan increased rapidly , but there were rapid fall in 2012, the balance gap increased obviously; the proportion of financial assistance increased in 2011, while the construction of income and expenditure remained relatively stable. Conclusion: The general economic operation efficiency of county-level hospitals improved, but the comprehensive reforms still has not appeared, which needs further implementation of compensation mechanism for ensuring the successful implementation of comprehensive reform.

Objective:To detect expression of tumor necrosis factor-converting enzyme(TACE)on the mouse uterine epithelial cell and effect of implantation in vitro. To analyze its role in blastocyst implantation and to investigate the relation TACE withimplantation of mouse embryo. Methods:Co-culturing endometrial epithelial cells and blastocyst of the pregnant 4-day mouse to establish the implantation model in vitro. Epithelial cells was identified by Immunocytochemistry. RT-PCR and Immunocytochemistry were used to detect the expression of TACE gene and protein in the endometrial epithelial cells. Different concentrations of TACE antibody were added to the implantation model to observe effect of TACE antibody on mouse blastocyst implantation in vitro. Expression of TACE protein was detected by Immunocytochemistry in each antibody interference system. Results:Endometrial epithelial cells growed vigorously in vitro. Keratin antibody showed positive expression,meanwhile,Vimentin antibody showed negative expression in the endometrial epithelial cells. PCR showed that the expression of TACE gene was high and results of Immunocytochemistry was consisted with PCR. The adhesion rate of mouse blastocyst was high in the co-culture system and decreased remarkably when TACE antibody was added (P

0.05)when taxol waswith a concentration of 6 ?g/ml and were 27.8% vs 42.0%(P

Objective:To evaluate the therapeutic effect of neoadjuvent chemotherapy for grade Ⅲ and Ⅳ carcinoma in the digestive tracts,Methods: A total of 30 cases of carcinoma in the digestive tracts were collected;including 9 cases in grade Ⅲa,13 cases in grade Ⅲb,and 8 cases in grade Ⅳ admitted to our hospital.Through gastroscope、enteroscope and CT investigation after admission,20 cases had been determined as advanced carcinoma in the digestive tracts before operation.Then the cases underwent chemotherapy twice,were rechecked by CT,and finally accepted operation treatment.The changes of the cancer volume,labelling micovessel index CD105 and the content of the inhibitory protein of apoptosis survivin from excisional tumor tissue were compared after operation. Results: 1.The change of tumorvolumebeforeandafterchemotherapyshowedstatisticalsignificance(P0.05)between intervenient chemotherapy group and systematical chemotherapy group. None died from operation. Conclusion: Surgical therapy after neoadjuvent chemotherapy for advanced cancer in the digestive tracts can improve the rate of exairesis and therapic effect.

Objective To study the effects of erythropoietin on expressions of myocardial cell Bcl-2 and Bax in rats with acute myocardial infarction(AMI).Methods Rats that survived after AMI were randomly divided into treatment group and control group. Treatment group were given EPO 3000 U/kg, A total of 3 d(preoperative 1 day, the same day, after 1 day),control group at the same point in time given the same amount of saline by the intraperitonealy injection. After 4 weeks, hemodynamics and left ventricular function were measured with catheterization,Bcl-2 and Bax were analyzed by immunohistochemical method. Results After 4 weeks, LVSP and +dp/dt_(max) in treatment group were significantly higher than those in control group(P＜0.05),LVEDP and-dp/dt_(max) were significantly lower than those in control group(P＜0.05);Change of heart rate in two groups were no difference(P＞0.05).The expression of myocardial cell Bcl-2 in treatment group were significantly higher than that in control group(P＜0.05);the expression of myocardial cell Bax and Bcl-2/Bax in treatment group were significantly lower than that in control group(P＜0.05).Conclusion The protective effect of EPO on ischemia and infarction of cardiac function may be related to the reduction of cardiomyocyte apoptosis.

Objective To know the preventive effects of chemophlebitis by lidocaine mortar. Methods Divided 77 patients with chemophhbitis into the observation group (42 cases) and the control group (35 cases) randomly. Lidocaine mortar was used in the observation group to prevent chemophlebitis, while the 50% MgH2SO4 was used in the control group. Compared the preventive efficacy between the two groups. Results There were 13 cases and 2 cases of chemophhbitis in the control group and the obser-vation group respectively. The incidence rate of chemophlebifis in the observation group was significant low-er than that of in the contrel group. The condition of chemotherapy-associated pains and continue time in the observation group were better than in the control group. Conclusions The preventive efficacy of chemophlebitis by lidocaine mortar is obviously.

ObjectiveTo give health guidance and intervention to Alzheimer's patients and observe its impact on their quality of life. MethodsBasic care, psychological care and safety of care measures were given to 45 Alzheimer's patients in our hospital from March 2007 to September 2008, self-care ability and cognitive ability were observed. ResultsThe Alzheimer's patients were alleviated in the following symptoms such as accidental aspiration, wrong intake, falls, self- injury, straying away, self- care ability, cognitive ability and social skills. ConclusionsStrengthening of diet and daily life care, attention to psy-chological and safety care in patients with Alzheimer's disease can improve their quality of life, which is worthy of hospital, community and family promotion.